Circulating microRNAs in malaria infection: bench to bedside

Chamnanchanunt, Supat; Fucharoen, Suthat; Umemura, Tomonari

doi:10.1186/s12936-017-1990-x

Cited by 33 publications

(30 citation statements)

References 79 publications

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“…Another study conducted on controlled human blood-stage infection (CHMI) has shown the dichotomous miRNA expression distinguishing high-miR responders from low-miR responders with significantly enhanced antimalarial antibody responses in the high-miR responders group [ 47 ]. At present there is no approved diagnostic modality available to detect the severity of the P. vivax malaria [ 48 ]. In the present study we identified the potential whole blood miRNA marker for the probable diagnosis of complicated P. vivax malaria using miRNA microarray.…”

Section: Discussionmentioning

confidence: 99%

“…However, microscopy is routinely being used for the diagnosis of malaria and remains the gold standard. Also rapid antigen detection test (RDT) and molecular tests (PCR and LAMP) have also been used for the diagnosis of malaria [ 13 ]. But we do not have any technique that can be used in order to differentiate the complicated P. vivax malaria from the uncomplicated P. vivax malaria.…”

Section: Introductionmentioning

confidence: 99%

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Screening and identification of potential novel biomarker for diagnosis of complicated Plasmodium vivax malaria

et al. 2018

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BackgroundIn the recent years Plasmodium vivax has been reported to cause severe infections associated with mortality. Clinical evaluation has limited accuracy for the early identification of the patients progressing towards the fatal condition. Researchers have tried to identify the serum and the plasma-based indicators of the severe malaria. Discovery of MicroRNA (miRNA) has opened up an era of identification of early biomarkers for various infectious and non-infectious diseases. MicroRNAs (miRNA) are the small non-coding RNA molecules of length 19–24 nts and are responsible for the regulation of the majority of human gene expressions at post transcriptional level.MethodsWe identified the differentially expressed miRNAs by microarray and validated the selected miRNAs by qRT-PCR. We assessed the diagnostic potential of these up-regulated miRNAs for complicated P. vivax malaria. Futher, the bioinformtic analysis was performed to construct protein–protein and mRNA–miRNA networks to identify highly regulated miRNA.ResultsIn the present study, utility of miRNA as potential biomarker of complicated P. vivax malaria was explored. A total of 276 miRNAs were found to be differentially expressed by miRNA microarray and out of which 5 miRNAs (hsa-miR-7977, hsa-miR-28-3p, hsa-miR-378-5p, hsa-miR-194-5p and hsa-miR-3667-5p) were found to be significantly up-regulated in complicated P. vivax malaria patients using qRT-PCR. The diagnostic potential of these 5 miRNAs were found to be significant with sensitivity and specificity of 60–71% and 69–81% respectively and area under curve (AUC) of 0.7 (p < 0.05). Moreover, in silico analysis of the common targets of up-regulated miRNAs revealed UBA52 and hsa-miR-7977 as majorly regulated hubs in the PPI and mRNA–miRNA networks, suggesting their putative role in complicated P. vivax malaria.ConclusionmiR-7977 might act as a potential biomarker for differentiating complicated P. vivax malaria from uncomplicated type. The elevated levels of miR-7977 may have a role to play in the disease pathology through UBA52 or TGF-beta signalling pathway.Electronic supplementary materialThe online version of this article (10.1186/s12967-018-1646-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening and identification of potential novel biomarker for diagnosis of complicated Plasmodium vivax malaria

et al. 2018

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“…Those findings provide the first evidence of the protective role from a specific miRNA in human malaria. Recently, it was shown that circulating levels of miR-451 and miR-16 were downregulated in patients infected with Plasmodium vivax at parasitic stages or with multi-organ failure involvement ( 25 , 52 ). This association of miRNAs profile with clinical severity of malaria infection emphasize that miRNAs are potential tools for diagnosis and therapeutics for parasitic diseases.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis Reveals That miR-193b, miR-671, and TREM-1 Correlate With a Good Response to Treatment of Human Localized Cutaneous Leishmaniasis Caused by Leishmania braziliensis

et al. 2018

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Localized cutaneous leishmaniasis (LCL) is a chronic disease characterized by ulcerated skin lesion(s) and uncontrolled inflammation. The mechanisms underlying the pathogenesis of LCL are not completely understood, and little is known about posttranscriptional regulation during LCL. MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression and can be implicated in the pathogenesis of LCL. We investigated the involvement of miRNAs and their targets genes in human LCL using publicly available transcriptome data sets followed by ex vivo validation. Initial analysis highlighted that miRNA expression is altered during LCL, as patients clustered separately from controls. Joint analysis identified eight high confidence miRNAs that had altered expression (−1.5 ≤ fold change ≥ 1.5; p < 0.05) between cutaneous ulcers and uninfected skin. We found that the expression of miR-193b and miR-671 are greatly associated with their target genes, CD40 and TNFR, indicating the important role of these miRNAs in the expression of genes related to the inflammatory response observed in LCL. In addition, network analysis revealed that miR-193b, miR-671, and TREM1 correlate only in patients who show faster wound healing (up to 59 days) and not in patients who require longer cure times (more than 60 days). Given that these miRNAs are associated with control of inflammation and healing time, our findings reveal that they might influence the pathogenesis and prognosis of LCL.

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“…The downregulated expression of miRNA‐451 and miRNA‐16 was due to the destruction of the RBCs containing miRNA by the parasite and hypersplenism that is destruction of the RBCs by spleen (LaMonte et al, ). MiRNA‐92 and miRNA‐17 were responsible for renal failure regulating the TGF‐β signaling, inducing apoptosis in the renal progenitor cells (Chamnanchanunt, Fucharoen, & Umemura, ). MiRNA‐92 and miRNA‐17 were responsible for renal failure regulating the TGF‐β signaling, inducing apoptosis in the renal progenitor cells (Chamnanchanunt et al, ).…”

Section: Role Of Circulatory Mirna For the Diagnosis Of Human Infectimentioning

confidence: 99%

Emerging role of circulating microRNA in the diagnosis of human infectious diseases

Ojha

Nandani

Pandey

et al. 2018

Journal Cellular Physiology

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The endogenic microRNAs (miRNA) are evolutionary, conserved, and belong to a group of small noncoding RNAs with a stretch of 19-24 nucleotides. The miRNAs play an indispensable role in gene modulation at the posttranscriptional level, inclusive of stem-cell differentiation, embryogenesis, hematopoiesis, metabolism, immune responses, or infections. The miRNAs secreted from the cells and their presence in the biological fluids signifies the regulatory role of circulating miRNAs in the pathogenesis. The phenomenal expression levels of circulating miRNAs in serum or plasma during infection makes them the potential therapeutic biomarkers for the diagnosis of assorted human infectious diseases. In this article, we have accentuated the methods for the profiling of circulating miRNA as well as the importance of miRNA as biomarkers for the diagnosis of human infectious diseases. To date, numerous biomarkers have been identified for the diagnostic or prognostic purpose; for instance, miR-182, miR-486, and miR15a in sepsis; miR-320 and miR505 in inflammatory bowel disease; miR-155 and miR-1260 in influenza; miR-12, miRVP-3p, and miR-184 in arboviruses; and miR-29b and miR-125 in hepatitis infection. Nevertheless, the noninvasive diagnostic approach, with the aid of biomarkers, currently plays a decisive role in the untimely diagnosis of human infections. So, in the near future, the exploitation of circulating miRNAs as therapeutic biomarkers for the diagnosis of human infections will help us to cure the associated diseases promptly and effectively.

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Circulating microRNAs in malaria infection: bench to bedside

Cited by 33 publications

References 79 publications

Screening and identification of potential novel biomarker for diagnosis of complicated Plasmodium vivax malaria

Screening and identification of potential novel biomarker for diagnosis of complicated Plasmodium vivax malaria

Integrated Analysis Reveals That miR-193b, miR-671, and TREM-1 Correlate With a Good Response to Treatment of Human Localized Cutaneous Leishmaniasis Caused by Leishmania braziliensis

Emerging role of circulating microRNA in the diagnosis of human infectious diseases

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