Circulating microRNAs in nonalcoholic fatty liver disease

DiStefano, Johanna K.; Gerhard, Glenn S.

doi:10.1586/17474124.2016.1125290

Cited by 27 publications

(18 citation statements)

References 27 publications

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“…Studies have suggested that miRNAs play a role in the development of NAFLD . Specifically, circulating miR‐122, miR‐192, and miR‐22 have been proposed as biomarkers of NASH . The miRNAs are positively correlated with plasma ALT levels and considered as better NASH biomarkers than ALT .…”

Section: Discussionmentioning

confidence: 99%

Blackcurrant (Ribes nigrum) Prevents Obesity‐Induced Nonalcoholic Steatohepatitis in Mice

Lee

Pham

Bae

et al. 2018

Obesity

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Objective: With increasing prevalence of nonalcoholic steatohepatitis (NASH), effective strategies to prevent NASH are needed. This study investigated whether the consumption of blackcurrant (Ribes nigrum) can prevent the development of obesity-induced NASH in vivo. Methods: Male C57BL/6J mice were fed a low-fat control diet, a low-fat diet with 6% whole blackcurrant powder, an obesogenic high-fat/high-sucrose control diet (HF), or a high-fat/high-sucrose diet containing 6% whole blackcurrant powder (HF-B) for 24 weeks. Results: HF significantly increased, whereas HF-B markedly decreased, liver weights and triglyceride. Furthermore, blackcurrant attenuated obesity-induced infiltration of macrophages in the liver, in particular, the M1 type, and also suppressed the hepatic expression of fibrogenic genes and fibrosis. Flow cytometric analysis showed that HF significantly increased the percentages of monocytes of total splenocytes, which was markedly attenuated by blackcurrant. HF-B decreased lipopolysaccharide-stimulated mRNA expression of interleukin 1β and tumor necrosis factor α in splenocytes, compared with those from HF controls. Moreover, the levels of circulating and hepatic miR-122-5p and miR-192-5p, known markers for nonalcoholic fatty liver disease, were significantly increased by HF but decreased by HF-B. Conclusions: The study's findings indicate that blackcurrant consumption prevents obesity-induced steatosis, inflammation, and fibrosis in the liver.

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Section: Discussionmentioning

confidence: 99%

Blackcurrant (Ribes nigrum) Prevents Obesity‐Induced Nonalcoholic Steatohepatitis in Mice

Lee

Pham

Bae

et al. 2018

Obesity

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“…80 Conversely, the investigation of urinary miRNAs in liver disorders has been performed only in the oncologic field.…”

Section: Livermentioning

confidence: 99%

Beyond circulating microRNA biomarkers: Urinary microRNAs in ovarian and breast cancer

et al. 2017

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Breast cancer is the most common malignancy in women worldwide, and ovarian cancer is the most lethal gynecological malignancy. Women carrying a BRCA1/2 mutation have a very high lifetime risk of developing breast and ovarian cancer. The only effective risk-reducing strategy in BRCA-mutated women is a prophylactic surgery with bilateral mastectomy and bilateral salpingo-oophorectomy. However, many women are reluctant to undergo these prophylactic surgeries due to a consequent mutilated body perception, unfulfilled family planning, and precocious menopause. In these patients, an effective screening strategy is available only for breast cancer, but it only consists in close radiological exams with a significant burden for the health system and a significant distress to the patients. No biomarkers have been shown to effectively detect breast and ovarian cancer at an early stage. MicroRNAs (miRNAs) are key regulatory molecules operating in a post-transcriptional regulation of gene expression. Aberrant expression of miRNAs has been documented in several pathological conditions, including solid tumors, suggesting their involvement in tumorigenesis. miRNAs can be detected in blood and urine and could be used as biomarkers in solid tumors. Encouraging results are emerging in gynecological malignancy as well, and suggest a different pattern of expression of miRNAs in biological fluids of breast and ovarian cancer patients as compared to healthy control. Aim of this study is to highlight the role of the urinary miRNAs which are specifically associated with cancer and to investigate their role in early diagnosis and in determining the prognosis in breast and ovarian cancer.

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“…Mi-RNAs, short, non-coding RNAs that regulate gene expression, have been associated with histological features of NAFLD and are readily detected in the circulation. [31][32][33][34][35] The current study showed a significant increase in the level of mi-RNA-122 in NAFLD patients compared to controls, and its level is associated with disease severity from simple steatosis to steatohepatitis. This finding is in agreement with Jampoka et al, 36 who found that serum mi-RNA-122 levels in NAFLD patients were significantly higher than those found in the control group.…”

Section: Discussionmentioning

confidence: 95%

<p>The Circulating Micro-RNAs (−122, −34a and −99a) as Predictive Biomarkers for Non-Alcoholic Fatty Liver Diseases</p>

Hendy

Rabie

Fouly

et al. 2019

DMSO

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Background: It remains essential for patient safety to develop non-invasive diagnostic tools to diagnose non-alcoholic fatty liver rather than invasive techniques. Aim: Our case-control study was to address the value of circulating miRNAs as a potential non-invasive biomarker for the diagnosis of non-alcoholic fatty acid diseases (NAFLD) and monitoring of disease progression. Methods: Routine clinical assessment, laboratory tests, anthropometric study, and liver biopsy results reported for 210 patients with NAFLD (124 patients of simple steatosis (SS) and 86 of non-alcoholic steatohepatitis (NASH)). Apparently matched for age and gender, healthy participants (n= 90) were enrolled as a control group. Serum samples were tested for micro-RNAs (−122, −34a and −99a) by quantitative-PCR. Results: By histopathology, 124 of the NAFLD group were of SS and 86 patients were of NASH. Compared with the control subjects, both mi-RNA-122 and −34a levels were increased in NAFLD (p< 001) and at a cutoff = 1.261, mi-RNA-122 had 92% sensitivity, 85% specificity to differentiate NAFLD from healthy controls, while mi-RNA-99a were significantly decreased in NAFLD patients with an observed decrease in disease severity, and at a cutoff = 0.46, miRNA-99a had 94% sensitivity and 96% specificity to discriminate SS from NASH. Conclusion: The integration of a circulating mi-RNA panel to diagnose NAFLD cases and to discriminate between SS and NASH. Large-scale study is still needed to verify the other mi-RNA profiles and their role in NAFLD pathogenesis and targeting therapy.

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Circulating microRNAs in nonalcoholic fatty liver disease

Cited by 27 publications

References 27 publications

Blackcurrant (Ribes nigrum) Prevents Obesity‐Induced Nonalcoholic Steatohepatitis in Mice

Blackcurrant (Ribes nigrum) Prevents Obesity‐Induced Nonalcoholic Steatohepatitis in Mice

Beyond circulating microRNA biomarkers: Urinary microRNAs in ovarian and breast cancer

<p>The Circulating Micro-RNAs (−122, −34a and −99a) as Predictive Biomarkers for Non-Alcoholic Fatty Liver Diseases</p>

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