Circulating miR-148b-3p and miR-27a-3p can be potential biomarkers for diagnosis of pre-diabetes and type 2 diabetes: integrating experimental and in-silico approaches

Abstract

Background In view of the growing global prevalence of type 2 diabetes (T2D), detection of prediabetes and type 2 diabetes in the early stages is necessary to reduce the risk of developing diabetes, prevent the progression of the disease, and dysfunction of different organs. Since miRNAs are involved in the initiation and progression of numerous pathogenic processes, including diabetes, in the present study, we aimed to investigate the expression of miR-148b-3p and miR-27a-3p in prediabetic and T2D patients and to evaluate the diagnostic potential of these miRNAs. Methods We evaluated the expression of miR-148b-3p and miR-27a-3p in the plasma of three groups: 20 prediabetic patients, 20 T2D patients, and 20 healthy controls. The biochemical parameters were determined by the auto-analyzer. The possible target genes of these miRNAs were identified using an in-silico approach. Results Our results showed that, as compared to the healthy controls, there was a significant up regulation and down regulation in the expression of miR-148b-3p and miR-27a-3p in the T2D patients, respectively. The results of receiver operating characteristic curve analysis also suggested that miR-148b-3p acted successfully in discriminating the prediabetic and diabetic patients from the control group. According to in-silico analysis, miRs influence biological pathways involved in T2DM development, such as insulin signaling. Conclusions The miR148b-3p and miR-27a-3p expression levels were deregulated in diabetes and pre-diabetes. Furthermore, miR-148b-3p showed significant ability in discriminating between diabetic and healthy individuals, suggesting a potential diagnostic use of miR-148b-3p in the detection of T2D.

Identification of MicroRNAs Associated with Prediabetic Status in Obese Women

“…It is a marker for different types of cancer [29,30] and was identified as potential synaptosomal miRNA upregulated with progression of Alzheimer's disease [31]. Of note, miR-23a [22], miR-148b-3p and miR-27a-3p [32], which have previously been implicated in prediabetes, were not differentially abundant in our study, possibly due to sex differences since the cohorts used in the other studies were largely male. Regarding a possible influence of sex hormones in our female cohort, we found indications that estrogen signaling may impinge on the expression of the candidate miRNAs.…”

MicroRNA for the early detection of type 2 diabetes mellitus: literature review

“…Se ha identificado que la desregulación de miR-148b-3p y miR-27a-3p está presente tanto en la diabetes como en la prediabetes, lo que respalda aún más su posible papel como biomarcadores de estas condiciones. (9) Además, Ramanjaneya et al, llevó a cabo una investigación comparativa de los niveles de miARNs en participantes con DMT2 y controles durante la hipoglicemia. Se observó que, en comparación con los controles, nueve de los diez miARNs con los mayores cambios desde el estado basal hasta la hipoglicemia mostraron diferencias significativas entre los participantes con DMT2 y los controles.…”

“…Se identificaron miARNs específicos, como miR-21, miR-122, miR-148b-3p, miR-27a-3p y miR-135, que mostraron niveles alterados en los pacientes estudiados. (9,6,13,11) Los estudios analizados han proporcionado evidencia sólida sobre el papel de los miARNs en la DMT2 y la prediabetes. Estos pequeños ARN reguladores están implicados en la señalización de insulina, la resistencia a la insulina, la inflamación y otros procesos metabólicos clave.…”

“…Se encontró que ciertos miARNs, como miR-21, miR122, miR-148b-3p y miR-135, podrían utilizarse como marcadores para la detección temprana de estas enfermedades, especialmente en individuos con antecedentes familiares de DMT2. (9,13)…”

To identify biomarkers associated with the transfer of diabetes combined with cancer in human genes using bioinformatics analysis

“…[5][6][7] Only a few pathogenic genes, such as MIR-21, Rfx2, HNF1A, Nus1, GP2, HBA 1c, MR-148B-3P, and MR-27A-3P, are thought to play significant roles in the development of metastasis in cancer patients with DM and associated disorders. [8][9][10][11][12][13] However, the relationship between these biomarkers and DM has not been adequately studied. Moreover, the differential expression of metastasis-related genes in patients with DM, with and without metastases, has not been studied.…”