Circulating miR-30b-5p levels in plasma as a novel potential biomarker for early detection of breast cancer

Adam‐Artigues, Anna; Garrido‐Cano, Iris; Simón, Soraya; Ortega, Belén; Moragón, Santiago; Lameirinhas, Ana; Constâncio, Vera; Salta, Sofia; Burgués, Octavio; Bermejo, Begoña; Henrique, Rui; Lluch, Ana; Jerónimo, Carmen; Eroles, Pîlar; Cejalvo, Juan Miguel

doi:10.1016/j.esmoop.2020.100039

Cited by 26 publications

(17 citation statements)

References 84 publications

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“…However, Wu et al 40 found that miR-30b-5p promoted the proliferation, migration and invasion of breast cancer cells. Moreover, the diagnostic and prognostic value of miR-30b-5p in peripheral blood and tumor tissues for renal cell carcinoma and breast cancer had been explored [41][42][43] . Our study demonstrated that hypoxic pancreatic cancer cells promoted angiogenesis through transferring exosomal miR-30b-5p to endothelial cells to regulate directly the expression of GJA1.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

Chen¹,

Wang²,

Liu³

et al. 2022

Int. J. Biol. Sci.

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Purpose: Most patients with pancreatic ductal adenocarcinoma (PDAC) have vascular invasion and metastasis, leading to low surgical resection rate and dismal prognosis. Tumor angiogenesis is related to vascular invasion and metastasis. However, anti-angiogenesis therapeutic effects in PDAC are limited. Therefore, it is imperative to explore molecular mechanism of angiogenesis in PDAC. Experimental Design: scRNA-seq data were utilized to delineatetranscriptional profiles of endothelial cells in PDAC. The in vitro and vivo angiogenesis models were used to explore the role of PDAC derived exosomes under hypoxic condition in tumor angiogenesis. Results: Endothelial cells in PDAC had distinct gene expression profiles compared with normal pancreas. The marker genes of endothelial cells in PDAC were enriched for hypoxia and angiogenesis. MiR-30b-5p were significantly enriched in hypoxic PDAC cells derived exosomes, which could be transferred to HUVEC, resulting in the upregulation of miR-30b-5p. Hypoxic PDAC cells derived exosomes could promote tube formation and endothelial cells migration via miR-30b-5p mediated downregulation of gap junction protein GJA1. Moreover, hypoxic PDAC cells derived exosomes increased new microvascular density in vivo. Patients with PDAC had higher levels of total miR-30b-5p and exosomal miR-30b-5p in peripheral blood plasma than healthy subjects. In addition, there were significant correlations for the levels of total miR-30b-5p or exosomal miR-30b-5p between peripheral blood plasma and portal vein plasma. Conclusions: Hypoxic PDAC cells derived exosomal miR-30b-5p promoted angiogenesis by inhibiting GJA1, and miR-30b-5p was a potential diagnostic marker for PDAC.

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Section: Discussionmentioning

confidence: 99%

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

Chen¹,

Wang²,

Liu³

et al. 2022

Int. J. Biol. Sci.

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“…43 In a study by Artigues for the first time on this subject; cir miRNA-30b-5p analysis in serum is the easiest to apply, noninvasive, highest sensitivity (78.3%) and specificity (72.3%), cost-effective diagnosis and screening method that can be effective in all types of BCs and early stages. 44 Despite these results, this study was not included among ADMs in our study because it was not performed in MBC cases.…”

Section: Resultsmentioning

confidence: 97%

What is the effect of advanced diagnostic methods on sensitivity and survival in the multiple breast cancers? A systematic analysis and comparison

Acar¹,

Ozer²

2021

Asian J Med Sci

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The sensitivities of the advanced diagnostic methods appear to be higher than the conventional methods and do have the potential to prolong survival, in multiple (multifocal and multicenter) breast cancers. Thus, the aim of this study is to compare the sensitivities of advanced and conventional diagnostic methods and to reveal their effects on survival. The studies published about diagnosis and treatment methods in multiple breast cancer in literature were searched, analyzed, and the sensitivities obtained with advanced and conventional diagnostic methods were compared, and the results were evaluated statistically. The sensitivity obtained with the advanced diagnostic methods in multiple breast cancers was found to be significantly higher than the conventional methods (p <0.05). The high sensitivities obtained with advanced diagnostic methods were found to have a high potential for survivial prolongation in multiple breast cancers. The use of advanced diagnostic methods in breast cancer screening programs, the investigation of tumor foci with molecular methods, increases the rate of diagnosis in multiple breast cancers. In the treatment of multiple breast cancers, a personalized treatment plan is made with the diagnosis of the molecular structure of the tumor foci. Thus, the recurrence rates are reduced, the survival is prolonged.

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“…Some of the circulating miRNAs summarized in Table 1 confirmed their clinical value by reflecting tumor stage, lymph node status, and metastases occurrence. For instance, miRNA-21, 199a, 210, and 221 were found to be associated with tumor stage, whereas expression of miRNA-10b, 17a, 30b, 93, 105, and 376c correlated with lymph node status and the presence of distant metastases [40,42,48,61,65]. Among the studied miRNAs, few molecules were identified as being significant for TNBC in independent studies, such as miRNA-21, miRNA-199a, and 489 [40,42,43,57].…”

Section: Mirnasmentioning

confidence: 98%

Blood Circulating Non-Coding RNAs for the Clinical Management of Triple-Negative Breast Cancer

Powrózek

Otieno

2022

Cancers

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Triple negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, and is related to unfavorable prognosis and limited treatment strategies. Currently, there is a lack of reliable biomarkers allowing for the clinical management of TNBC. This is probably caused by a complex molecular background, leading to the development and establishment of a unique tumor phenotype. Recent studies have reported non-coding RNAs (ncRNAs) not only as the most promising class of molecular agents with a high applicability to manage human cancers, including TNBC, but also as robust and non-invasive biomarkers that are able to be monitored in blood circulation, with the application of liquid biopsy. There is a lack of papers discussing the role of blood-circulating ncRNAs as diagnostic, predictive, and prognostic biomarkers for TNBC. In this paper, we summarized the available literature reports on the utility of blood-circulating ncRNAs for TNBC management. Additionally, we supplemented this review by bioinformatics analysis, for better understanding of the role of ncRNAs’ machinery in the development of a unique TNBC phenotype.

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Circulating miR-30b-5p levels in plasma as a novel potential biomarker for early detection of breast cancer

Cited by 26 publications

References 84 publications

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression

What is the effect of advanced diagnostic methods on sensitivity and survival in the multiple breast cancers? A systematic analysis and comparison

Blood Circulating Non-Coding RNAs for the Clinical Management of Triple-Negative Breast Cancer

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