Circulating miR-3613-5p but not miR-125b-5p, miR-199a-3p, and miR-451a are biomarkers of endometriosis

Walasik, Izabela; Klicka, Klaudia; Grzywa, Tomasz M.; Szymusik, Iwona; Włodarski, Paweł; Wielgoś, Mirosław; Pietrzak, Bronisława; Ludwin, Artur

doi:10.1016/j.repbio.2023.100796

Cited by 5 publications

(9 citation statements)

References 29 publications

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“…Fourteen studies included patients with endometriosis stage I to IV based on the ASRM classification [20,25,26,28,30,31,34,[38][39][40][41][42][43]49]. Women with moderate-to-severe endometriosis (stage III to IV) were included in ten studies [22,23,27,37,44,45,47,48,50,51]. Six studies did not mention the stage of endometriosis [29,32,33,35,36,46], and the remaining two studies included only women with stage I to II [24] and II to IV [21], respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The control groups consisted of self-reported healthy women or patients with symptoms suggestive of endometriosis which could not be confirmed or women with other benign gynecological conditions. The majority of the studies included patients with no use of hormonal treatment in the last 3 months [20,[22][23][24][25][26][27]31,32,34,35,37,38,42,43,45,47,50,51]; Gu et al [46] and Bahramy et al [48] even decided to not include patients who took hormonal treatment during the last 6 months. Ten studies did not specify the hormonal treatment of the included patients [21,[29][30][31]33,36,39,40,44,49].…”

Section: Resultsmentioning

confidence: 99%

“…A diverse study population was used in Pateisky et al [41], Nisenblat et al [42] and Moustafa et al [28], with both women who were taking hormones at the time of the study and women who were not taking them, as well as patients in whom this was uncertain. The menstrual phase was not specified in seventeen studies [20][21][22]26,[29][30][31][33][34][35][36]39,40,44,45,49,51]. Eleven studies made a distinction between the proliferative phase and the secretory phase [23][24][25]27,28,37,38,41,[46][47][48].…”

Section: Resultsmentioning

confidence: 99%

“…Sixteen studies investigated the presence of miRNA in serum [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], and an equal number of studies did so in plasma [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]. In some cases, the selection of miRNAs was conducted based on a literature review of existing studies [22,[25][26][27][28][29][30][31][32]34,35,39,40,44,47,48,51]. Two studies used online databases for targeted analysis to predict miRNAs' target genes…”

Section: Resultsmentioning

confidence: 99%

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Circulating microRNAs as Non-Invasive Biomarkers in Endometriosis Diagnosis—A Systematic Review

Vanhie,

Caron,

Vermeersch

et al. 2024

Biomedicines

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The aim of this systematic review is to assess the power of circulating miRNAs as biomarkers as a diagnostic tool in endometriosis. In endometriosis-suspected women with uncertain imaging, the only way to confirm or exclude endometriosis with certainty is currently laparoscopy. This creates a need for non-invasive diagnostics. We searched the literature through the PubMed database using the Mesh terms ‘endometriosis’ and ‘miRNAs’. Some, but limited, overlap was found between the 32 articles included, with a total of 20 miRNAs reported as dysregulated in endometriosis in two or more studies. MiR-17-5p was reported as dysregulated in six studies, followed by miR-451a and let-7b-5p in four studies and miR-20a-5p, miR-143-3p, miR-199a-5p and miR-3613-5p in three studies. Furthermore, a possible impact of the menstrual phase on miRNA expression was noted in five studies, while no influence of hormonal intake was observed in any included study. The modest reproducibility between studies may be attributable to biological variability as well as to the lack of universal protocols, resulting in pre- and analytical variability. Despite the identification of several suitable candidate biomarkers among the miRNAs, the need for high-quality studies with larger and well-defined population cohorts and the use of standardized protocols lingers.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Circulating microRNAs as Non-Invasive Biomarkers in Endometriosis Diagnosis—A Systematic Review

Vanhie,

Caron,

Vermeersch

et al. 2024

Biomedicines

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“…Previous studies investigating miRNA levels in blood samples from endometriosis patients have reported varying results ( 10 – 12 ). Of the more recent studies, multiple blood-based miRNAs have been included in diagnostic models of endometriosis; however, none of the potential diagnostic miRNAs identified overlapped between studies ( 17 – 20 , 22 , 53 – 56 ). Vanhie et al (2019) ( 54 ), Nisenblat et al (2019) ( 22 ), and Papari et al (2020) ( 18 ) have assessed miRNA diagnostic models for endometriosis among independent validation cohorts.…”

Section: Discussionmentioning

confidence: 99%

Plasma microRNA expression in adolescents and young adults with endometriosis: the importance of hormone use

Brady,

Yousif,

Sasamoto

et al. 2024

Front. Reprod. Health

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IntroductionPrior studies have investigated the diagnostic potential of microRNA (miRNA) expression profiles for endometriosis. However, the vast majority of previous studies have only included adult women. Therefore, we sought to investigate differential expression of miRNAs among adolescents and young adults with endometriosis.MethodsThe Women's Health Study: from Adolescence to Adulthood (A2A) is an ongoing WERF EPHect compliant longitudinal cohort. Our analysis included 64 patients with surgically-confirmed endometriosis (96% rASRM stage I/II) and 118 females never diagnosed with endometriosis frequency matched on age (median = 21 years) and hormone use at blood draw. MicroRNA measurement was separated into discovery (10 cases and 10 controls) and internal replication (54 cases and 108 controls) phases. The levels of 754 plasma miRNAs were assayed in the discovery phase using PCR with rigorous internal control measures, with the relative expression of miRNA among cases vs. controls calculated using the 2−ΔΔCt method. miRNAs that were significant in univariate analyses stratified by hormone use were included in the internal replication phase. The internal replication phase was split 2:1 into a training and testing set and utilized FirePlex miRNA assay to assess 63 miRNAs in neural network analyses. The testing set of the validation phase was utilized to calculate the area under the curve (AUC) of the best fit models from the training set including hormone use as a covariate.ResultsIn the discovery phase, 49 miRNAs were differentially expressed between endometriosis cases and controls. The associations of the 49 miRNAs differed by hormone use at the time of blood draw. Neural network analysis in the testing set of the internal replication phase determined a final model comprising 5 miRNAs (miR-542-3p, let-7b-3p, miR-548i, miR-769-5p, miR-30c-1-3p), yielding AUC = 0.77 (95% CI: 0.67–0.87, p < 0.001). Sensitivity in the testing dataset improved (83.3% vs. 72.2%) while the specificity decreased (58.3% vs. 72.2%) compared to the training set.ConclusionThe results suggest that miR-542-3p, let-7b-3p, miR-548i, miR-769-5p, miR-30c-1-3p may be dysregulated among adolescent and young adults with endometriosis. Hormone use was a significant modifier of miRNA dysregulation and should be considered rigorously in miRNA diagnostic studies.

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miR-375-3p predicts the severity of endometriosis and regulates cellular progression by targeting NOX4

Wang,

Li,

Han

et al. 2025

Molecular and Cellular Probes

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Circulating miR-3613-5p but not miR-125b-5p, miR-199a-3p, and miR-451a are biomarkers of endometriosis

Cited by 5 publications

References 29 publications

Circulating microRNAs as Non-Invasive Biomarkers in Endometriosis Diagnosis—A Systematic Review

Circulating microRNAs as Non-Invasive Biomarkers in Endometriosis Diagnosis—A Systematic Review

Plasma microRNA expression in adolescents and young adults with endometriosis: the importance of hormone use

miR-375-3p predicts the severity of endometriosis and regulates cellular progression by targeting NOX4

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