Circulating MiRNA-21 and programed cell death (PDCD) 4 gene expression in hepatocellular carcinoma (HCC) in Egyptian patients

Gedawy, Gamalat El; Obada, Manar; Kelani, Ayman; El-Said, Hala; Ghanayem, Naglaa M

doi:10.1016/j.ejmhg.2016.04.007

Cited by 11 publications

(10 citation statements)

References 44 publications

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“…In our study we found that the plasma level of miRNA 21 was significantly higher in liver cirrhosis and HCC groups in comparison to the healthy control group, being highest among HCC patients compared to the other two groups with p-value < 0.001. Our results were similar to Guo et al [16], El Gedawy et al [17], Karakatsanis et al [18], and Demerdash et al [19].…”

Section: Discussionsupporting

confidence: 92%

“…They reported that AFP showed lower sensitivity and specificity and suggested that miRNA-21 could have a greater diagnostic performance in discriminating HCC, liver cirrhosis, and chronic hepatitis than AFP [16], while in our study, the sensitivity of miRNA 21 was lower than AFP (68% for miRNA 21 compared to 84%), but with higher specificity. El Gedawy et al showed that miRNA-21 revealed that, at a cut-off value of 3.93 (fold expression), the sensitivity and specificity for differentiation of HCC cases were 93% and 90%, respectively [17]. Hegazy et al found that the specificity of miRNA-21 was superior to AFP in diagnosis of HCC [21].…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA 21as a novel biomarker in hepatitis C virus-related hepatocellular carcinoma

et al. 2022

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Background Hepatocellular carcinoma is considered one of the most common cancers occurring in human population all over the world. It became an increasingly threatening malignancy due to both morbidity and mortality. Chronic viral hepatitis B and hepatitis C are two risk factors, which account for 80–90% of all HCC cases worldwide. Alfa Feto protien is used as a tumor marker for HCC diagnosis and prognosis prediction; however, its false negative rate when used alone is as high as 40% for patients with early-stage HCC. AFP levels remain normal in 15–30% of all the patients, even patients with advanced HCC. It has been demonstrated that miRNAs (MicroRNAs) are an important class of non-coding RNAs. They act as tumor oncogenes or suppressors and are involved in the HCC development. MiRNAs are endogenous nucleotides that can be found in intra- and extracellular spaces, such as the blood, urine, and saliva. The study evaluated the miRNA 21 as a novel biomarker in patients with HCV related hepatocellular carcinoma. Results The study was conducted on three groups. Group (1) included 25 patients with liver cirrhosis due to hepatitis C virus infection. Group (2) included 25 patients with hepatocellular carcinoma (HCC) on top of liver cirrhosis due to hepatitis C virus infection. Group (3) included 10 normal control subjects. There was a significant difference in the mean level of miRNA between the three groups with p value < 0.001 with the highest value in group 2 ( 8.28 ± 2.55), then in group1 (5.04 ± 2.11) and the lowest in group 3 (control) (1.02 ± 0.07). MiRNA 21 has a sensitivity of 68% and a specificity of 96%, to differentiate between the liver cirrhosis group and HCC group. Conclusion miRNA 21 can be a promising marker for detection of patients with HCV-related hepatocellular carcinoma, with higher specificity compared to α feto protein; however, its cost is higher.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

MicroRNA 21as a novel biomarker in hepatitis C virus-related hepatocellular carcinoma

et al. 2022

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“…Furthermore, increased expression of miR-21 in HCC group would be due to the fact that miR-21 functions as an oncogene and its upregulation promotes malignant cell proliferation and invasion and contributes to evasion of the host immune system [ 29 ], and most HCC patients in this study were BCLC stages A and B. Likewise, El Gedawy et al reported that elevated plasma miR-21 stimulated cell migration and invasion by targeting PTEN (phosphatase and tensin enzyme; part of a chemical pathway that inhibits cell division and triggers apoptosis) and PDCD4 (programmed cell death 4; a tumor suppressor gene) and it could also directly target MAP2K3 (mitogen-activated protein kinase 3) [ 31 ].…”

Section: Discussionmentioning

confidence: 89%

“…Likewise, El Gedawy et al reported that elevated plasma miR-21 stimulated cell migration and invasion by targeting PTEN (phosphatase and tensin enzyme; part of a chemical pathway that inhibits cell division and triggers apoptosis) and PDCD4 (programmed cell death 4; a tumor suppressor gene) and it could also directly target MAP2K3 (mitogen-activated protein kinase 3) [31]. …”

Section: Discussionmentioning

confidence: 99%

Detection of MicroRNA in Hepatic Cirrhosis and Hepatocellular Carcinoma in Hepatitis C Genotype-4 in Egyptian Patients

Demerdash

Hussien

Hassouna

et al. 2017

BioMed Research International

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Background In Egypt, the prevalence of chronic hepatitis C (CHC) infection is 13.8% of whole population and about 80% of the patients with hepatocellular carcinoma have underling hepatitis C. Aim This study was designed to assess the diagnostic value of plasma miR-122 and miR-21 in patients with CHC, genotype-4, to detect fibrosis progression versus noninvasive indices and their diagnostic value in detection of early stages of hepatocellular carcinoma (HCC). Methodology A prospective study that included 180 patients, divided into 3 groups: healthy controls (group I), CHC patients (group II), and hepatitis C patients with HCC (group III); all cases were subjected to thorough clinical, radiological, and laboratory investigations. Selected biomarkers were evaluated and correlated with degree of liver damage. Results revealed that miR-122 followed by miR-21 had the highest efficiency in prediction of liver cell damage. Also, miR-21 was strongly correlated with vascular endothelial growth factor (VEGF) and alpha fetoprotein (α-FP) in HCC patients. Conclusions Plasma miR-122 and miR-21 had strong correlation with degree fibrosis in HCV genotype-4 patients; consequently they can be considered as potential biomarker for early detection of hepatic fibrosis. Moreover, miR-21 can be used as a potential biomarker, for early detection of HCC combined with VEGF and α-FP.

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“…One of such promising molecules is miR-21 which is a representative oncogenic miR so called "onco-miR". miR-21 is a 22 nucleotides-long molecule that drives cancer development by targeting numerous tumor suppressor genes associated with proliferation, apoptosis and invasion including PTEN [36], PDCD4 [37,38], and TPM1 [39]. miR-21 is overexpressed in various types of tumors.…”

Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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Micro RNA (miR) are regulatory non-coding RNA molecules, which contain a small number of nucleotides ~18–28 nt. There are many various miR sequences found in plants and animals that perform important functions in developmental, metabolic, and disease processes. miRs can bind to complementary sequences within mRNA molecules thus silencing mRNA. Other functions include cardiovascular and neural development, stem cell differentiation, apoptosis, and tumors. In tumors, some miRs can function as oncogenes, others as tumor suppressors. Levels of certain miR molecules reflect cellular events, both normal and pathological. Therefore, miR molecules can be used as biomarkers for disease diagnosis and prognosis. One of these promising molecules is miR-21, which can serve as a biomarker with high potential for early diagnosis of various types of cancer. Here, we present a novel design of miR detection and demonstrate its efficacy on miR-21. The design employs emissive properties of DNA-silver nanoclusters (DNA/AgNC). The detection probe is designed as a hairpin DNA structure with one side of the stem complimentary to miR molecule. The binding of target miR-21 opens the hairpin structure, dramatically modulating emissive properties of AgNC hosted by the C12 loop of the hairpin. “Red” fluorescence of the DNA/AgNC probe is diminished in the presence of the target miR. At the same time, “green” fluorescence is activated and its intensity increases several-fold. The increase in intensity of “green” fluorescence is strong enough to detect the presence of miR-21. The intensity change follows the concentration dependence of the target miR present in a sample, which provides the basis of developing a new, simple probe for miR detection. The detection strategy is specific, as demonstrated using the response of the DNA/AgNC probe towards the scrambled miR-21 sequence and miR-25 molecule. Additionally, the design reported here is very sensitive with an estimated detection limit at ~1 picomole of miR-21.

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Circulating MiRNA-21 and programed cell death (PDCD) 4 gene expression in hepatocellular carcinoma (HCC) in Egyptian patients

Cited by 11 publications

References 44 publications

MicroRNA 21as a novel biomarker in hepatitis C virus-related hepatocellular carcinoma

MicroRNA 21as a novel biomarker in hepatitis C virus-related hepatocellular carcinoma

Detection of MicroRNA in Hepatic Cirrhosis and Hepatocellular Carcinoma in Hepatitis C Genotype-4 in Egyptian Patients

Micro RNA Sensing with Green Emitting Silver Nanoclusters

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