Circulating miRNAs in nontumoral liver diseases

Amaral, Alex Evangelista do; Cisilotto, Júlia; Creczynski-Pasa, Tânia Beatriz; Schiavon, Leonardo de Lucca

doi:10.1016/j.phrs.2017.10.002

Cited by 20 publications

(14 citation statements)

References 112 publications

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“…Based on a literature review [ 8 , 11 , 12 ], the eight most interesting miRNAs were selected. The criteria for selection were either high expression or specificity to human liver and/or critical functions in liver physiology or pathology; all criteria are summarized in Material and method section below.…”

Section: Resultsmentioning

confidence: 99%

“…Not to mention that PCLS allow us to test toxicity and expression changes for multiple hepatotoxicants at the same time and from a single liver donor. Based on a literature review [ 8 , 11 , 12 ], eight interesting miRNAs were selected. The criteria for selection were either high expression or specificity to human liver and/or critical functions in liver physiology or pathology (Material and methods section).…”

Section: Introductionmentioning

confidence: 99%

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Inter-Individual Variability in Acute Toxicity of R-Pulegone and R-Menthofuran in Human Liver Slices and Their Influence on miRNA Expression Changes in Comparison to Acetaminophen

Zárybnický

Matoušková

Lancošová

et al. 2018

IJMS

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Monoterpenes R-pulegone (PUL) and R-menthofuran (MF), abundant in the Lamiaceae family, are frequently used in herb and food products. Although their hepatotoxicity was shown in rodent species, information about their effects in human liver has been limited. The aim of our study was to test the effects of PUL, MF and acetaminophen (APAP, as a reference compound) on cell viability and microRNA (miRNA) expression in human precision-cut liver slices. Slices from five patients were used to follow up on the inter-individual variability. PUL was toxic in all liver samples (the half-maximal effective concentration was 4.0 µg/mg of tissue), while MF and surprisingly APAP only in two and three liver samples, respectively. PUL also changed miRNA expression more significantly than MF and APAP. The most pronounced effect was a marked decrease of miR-155-5p expression caused by PUL even in non-toxic concentrations in all five liver samples. Our results showed that PUL is much more toxic than MF and APAP in human liver and that miR-155-5p could be a good marker of PUL early hepatotoxicity. Marked inter-individual variabilities in all our results demonstrate the high probability of significant differences in the hepatotoxicity of tested compounds among people.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inter-Individual Variability in Acute Toxicity of R-Pulegone and R-Menthofuran in Human Liver Slices and Their Influence on miRNA Expression Changes in Comparison to Acetaminophen

Zárybnický

Matoušková

Lancošová

et al. 2018

IJMS

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“…The sequential progression from fibrosis to cirrhosis results in dysplastic nodules, finally culminating into HCC. Several research groups have analyzed single miRNAs or miRNA panel with a diagnostic potential for liver fibrosis in patients with chronic liver diseases (summarized e.g., in Roy et al, 2015 ; Loosen et al, 2017 ; do Amaral et al, 2018 ). Notably, for some of these miRNAs (e.g., miR-22, miR-34, and miR-122), it could be demonstrated that their diagnostic value of is superior to that of classical markers including alanine aminotransferase, CK-18, FIB-4 and APRI, highlighting the potential of miRNAs in the context of liver diseases ( Anadol et al, 2015 ; Appourchaux et al, 2016 ; Liu et al, 2016 ).…”

Section: Circulating Mirnamentioning

confidence: 99%

A General Overview on Non-coding RNA-Based Diagnostic and Therapeutic Approaches for Liver Diseases

2018

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Liver diseases contribute to the global mortality and morbidity and still represent a major health problem leading to the death of people worldwide. Although there are several treatment options available for Hepatitis C infections, for most liver disease the pharmacological options are still limited. Therefore, the development of new targets against liver diseases is of high interest. Non-coding RNA (ncRNA) such as microRNA (miRNA) or long ncRNA (lncRNA) have been shown to be deeply involved in the pathophysiology of almost all acute and chronic liver diseases. The emerging evidence showed the potential therapeutic use of miRNA associated with different steps of hepatic pathophysiology. In the present review, we summarize emerging insights of ncRNA in liver diseases. We also highlight example of ncRNAs participating in the pathogenesis of different forms of liver disease and how they can be used as potential therapeutic targets for novel treatment paradigms. Furthermore, we describe an overview of up-to-date clinical trials and discuss about its future in clinical applications. Finally, we highlight the role of circulating ncRNAs in diagnosis of liver diseases and discuss the challenges and drawbacks of the usage of ncRNAs in clinical setting.

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“…In addition, miR-377 suppresses NSCLC by decreasing the expression of astrocyte elevated gene-1 (12). Circulating miRNAs, including those in serum and plasma, have been demonstrated to serve as potential noninvasive biomarkers for the early identification of various types of cancer due to their long-term stability in the circulatory system (13,14). In NSCLC, the upregulation of plasma miR-21 has been demonstrated to be effective for its early detection and chemosensitivity (15).…”

Section: Introductionmentioning

confidence: 99%

High serum miR‑484 expression is associated with the diagnosis and prognosis of patients with non‑small cell lung cancer

2019

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The aim of the current study was to assess the expression and clinical significance of serum microRNA (miR)-484 in patients with non-small cell lung cancer (NSCLC). Reverse transcription-quantitative polymerase chain reaction was performed to determine the expression of miR-484 in the serum of patients with NSCLC and NSCLC cell lines. Cell counting kit-8, flow cytometry, cell migration and cell invasion assays were performed to assess the role of miR-484 in the malignant changes associated with NSCLC cells. Furthermore, to assess the diagnostic value of miR-484, receiver operating curve (ROC) analysis was performed and the clinical relevance of serum miR-484 expression in patients with NSCLC was determined. A Kaplan-Meier analysis with the log-rank test was performed to assess the overall survival rate patients. To the best of our knowledge, the current study demonstrates for the first time that serum miR-484 was increased in patients with NSCLC compared with healthy controls. Additionally, serum miR-484 was revealed to be positively associated with histological grade, lymph node metastasis, distant metastasis and clinical stage. Patients with NSCLC and high serum miR-484 levels demonstrated significantly poorer overall survival rates compared with those exhibiting lower serum miR-484 expressions. ROC analysis revealed that serum miR-484 could screen patients with NSCLC patients from healthy controls with a high sensitivity and specificity. In vitro analysis also demonstrated that miR-484 was significantly upregulated in NSCLC cell lines, including 95D and H358 cells. Furthermore, the suppression of miR-484 decreased cell proliferation, cell migration and invasion. In summary, the results of the present study demonstrated that increased serum miR-484 expression is associated with the diagnosis and prognosis of patients with NSCLC. High serum miR-484 expression is associated with the diagnosis and prognosis of patients with non-small cell lung cancer

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Circulating miRNAs in nontumoral liver diseases

Cited by 20 publications

References 112 publications

Inter-Individual Variability in Acute Toxicity of R-Pulegone and R-Menthofuran in Human Liver Slices and Their Influence on miRNA Expression Changes in Comparison to Acetaminophen

Inter-Individual Variability in Acute Toxicity of R-Pulegone and R-Menthofuran in Human Liver Slices and Their Influence on miRNA Expression Changes in Comparison to Acetaminophen

A General Overview on Non-coding RNA-Based Diagnostic and Therapeutic Approaches for Liver Diseases

High serum miR‑484 expression is associated with the diagnosis and prognosis of patients with non‑small cell lung cancer

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