Circulating osteopontin and its association with liver fat content in non-obese women with polycystic ovary syndrome: a case control study

Abstract

BackgroundOsteopontin (OPN) plays an important role in inflammatory processes and insulin resistance. Polycystic ovary syndrome (PCOS) is a reproductive metabolic disease associated with insulin resistance and metabolic abnormalities, including high levels of liver fat content (LFC). The objective of this study was to explore whether circulating OPN independently contributes to elevated LFC in non-obese PCOS patients.MethodsThis study included 61 non-obese PCOS patients and 56 age-matched healthy women from Shanghai, China. After an overnight fast, all participants underwent anthropometric measurements, oral glucose tolerance tests, lipid profile and sex hormone measurements. Quantitative measurement of LFC by ultrasonography was performed. OPN concentrations were measured using ELISA. An independent samples t-test and the Mann-Whitney U test were performed to compare variables between the two groups; one-way ANOVA and Kruskal-Wallis test were performed to compare four subgroups of patients. Correlations were determined by Spearman’s correlation tests. Stepwise multiple linear regression analyses were performed to assess for independent contributors. A receiver operating characteristic curve with the maximum Youden index was calculated for the optimal cut-off value.ResultsIn non-obese PCOS women, circulating OPN levels were increased in the subgroups with a higher body mass index (BMI) and free androgen index (FAI), and the LFC levels were increased in the elevated OPN subgroups. Moreover, increased OPN was associated with increased FAI and LFC in PCOS women, and the association between OPN and LFC was independent of triglyceride, HOMA-IR and FAI after adjusting for PCOS status in all participants. OPN combined with FAI and hsCRP may better predict NAFLD than WHR in this study cohort. However, there was no significant difference in circulating OPN levels between non-obese PCOS and normal control women.ConclusionsIncreased OPN levels may be related to FAI and elevated LFC in non-obese women with PCOS.Electronic supplementary materialThe online version of this article (10.1186/s12958-018-0331-4) contains supplementary material, which is available to authorized users.

WITHDRAWN: Exploring Adipsin, Irisin, and Osteopontin as Biomarkers in Polycystic Ovary Syndrome and Their Associations with Metabolic Alterations: A prospective, case-control study

“…Serum osteopontin levels were determined by the sandwich ELISA method (Elabscience, Catalog number: E-EL-H1347, USA). The intra-assay CV and the inter-assay CVS were < 4.1% and < 6.7% (15).…”

“…Following the overnight fasting, we obtained blood samples from each patient in Groups PCOS and Control in the morning during the second or third day of their menstrual cycle for laboratory investigations, including glucose, cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride, insulin, the glycated hemoglobin (HbA1c), C-reactive protein (CRP), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), prolactin, total testosterone, and thyroid stimulating hormone (TSH). We calculated the homeostasis assessment insulin resistance index (HOMA-IR) using fasting serum insulin and fasting plasma glucose as described (15). The triglyceride/glucose index was calculated with the relevant measurements for each patient (20).…”

“…As an extracellular matrix-associated phosphoprotein, osteopontin plays a role in macrophage recruitment to in ammation in adipose tissue, contributing to chronic low-grade in ammation (14,15). So, induction of in ammatory signaling via osteopontin might impair differentiation and insulin sensitivity of primary adipocytes (15). Nevertheless, the association between osteopontin and PCOS has been studied in selected populations with con icting outcomes (14)(15)(16)(17).…”

“…So, induction of in ammatory signaling via osteopontin might impair differentiation and insulin sensitivity of primary adipocytes (15). Nevertheless, the association between osteopontin and PCOS has been studied in selected populations with con icting outcomes (14)(15)(16)(17).…”

Comparison of serum adiponectin and osteopontin levels along with metabolic risk factors between obese and lean women with and without PCOS

“…In relation to macrophage infiltration and inflammation, OPN is also demonstrated to associate in the pathogenesis of atherosclerotic process, insulin resistance, and malignant disorders [19]. Recently only a few studies have indicated that increased osteopontin levels may be associated with PCOS in selected populations [14,21].…”