Circulating precursors of human CD1c+ and CD141+ dendritic cells

Breton, Gaëlle; Lee, Jaeyop; Zhou, Yu; Schreiber, Joseph J.; Keler, Tibor; Puhr, Sarah; Anandasabapathy, Niroshana; Schlesinger, Sarah J.; Caskey, Marina; Liu, Kang; Nussenzweig, Michel C.

doi:10.1084/jem.20141441

Cited by 185 publications

(197 citation statements)

References 51 publications

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“…34,35 This population was detected in the BM of BRGSF mice (supplemental Figure 2B), and its frequency and total numbers increased following Flt3L treatment (supplemental Figure 2C). These data suggest that the increased frequency and absolute numbers of myeloid cells in Flt3L-treated BRGSF HIS mice may result from an increase in the CD34…”

Section: Resultsmentioning

confidence: 99%

A functional DC cross talk promotes human ILC homeostasis in humanized mice

et al. 2017

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Section: Resultsmentioning

confidence: 99%

A functional DC cross talk promotes human ILC homeostasis in humanized mice

et al. 2017

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“…This approach is based on an accelerated dendritic cell (DC) coculture system, designed for the optimal activation of antigen‐specific T‐cells from PBMCs (Martinuzzi et al ., 2011). As circulating human DCs are rare, DC precursors within the starting PBMC material were mobilized using FLT3 ligand (FLT3L) (Breton et al ., 2015), known for its capacity to enhance the induction of T‐cell responses (Guermonprez et al ., 2013), and matured with a standard cocktail of inflammatory cytokines (TNF, IL‐1β, PGE2, and IL‐7).Furthermore, we focused on the priming of CD8 + T‐cells specific for the melanoma antigen Melan‐A/MART‐1, restricted by HLA‐A*0201 (HLA‐A2 from hereon). This specificity is particularly amenable to in vitro studies with limited volume blood samples due to naturally high precursor frequencies in the naive pool and the widespread occurrrence of HLA‐A2 in the general population.…”

Section: Introductionmentioning

confidence: 99%

Reduced naïve CD 8 ⁺ T ‐cell priming efficacy in elderly adults

et al. 2015

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SummaryAging is associated with impaired vaccine efficacy and increased susceptibility to infectious and malignant diseases. CD8+ T‐cells are key players in the immune response against pathogens and tumors. In aged mice, the dwindling naïve CD8+ T‐cell compartment is thought to compromise the induction of de novo immune responses, but no experimental evidence is yet available in humans. Here, we used an original in vitro assay based on an accelerated dendritic cell coculture system in unfractioned peripheral blood mononuclear cells to examine CD8+ T‐cell priming efficacy in human volunteers. Using this approach, we report that old individuals consistently mount quantitatively and qualitatively impaired de novo CD8+ T‐cell responses specific for a model antigen. Reduced CD8+ T‐cell priming capacity in vitro was further associated with poor primary immune responsiveness in vivo. This immune deficit likely arises as a consequence of intrinsic cellular defects and a reduction in the size of the naïve CD8+ T‐cell pool. Collectively, these findings provide new insights into the cellular immune insufficiencies that accompany human aging.

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“…Intermediate CD14 + CD16 + monocytes have been also identified but are less well characterized functionally [4]. Precursors of conventional CD1c + and CD141 + DCs, as well as plasmacytoid DCs and activated HLA-DR + neutrophils, can be also identified in human blood [7,8]. The kinetics and phenotype of these APCs may be used to evaluate human immune responses to pathogens and vaccines [9].…”

mentioning

confidence: 99%