Circulating Progenitor Cell Response to Exercise in Wheelchair Racing Athletes

Abstract

These data suggest that CPC content in wheelchair racing athletes is related to cardiorespiratory fitness, and responses to exercise are positively related to exercise intensity.

Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults

“…2017). Our baseline cross‐sectional analysis aligns with these previous data, as well as data from elite wheelchair athletes (Niemiro et al . 2018), showing that cardiorespiratory fitness is positively associated with HSPCs.…”

“…The EDTA‐anticoagulant tubes were used for flow cytometry analysis as previously described (Niemiro et al . 2017, ). Briefly, PBMCs were isolated using Ficoll‐Paque Plus (GE Life Sciences) density gradient, per manufacturer's instructions.…”

“…CPCs were quantified using previously defined protocols (Niemiro et al . 2017, ). Antibodies for CPC (CD34 + ), HSPC (CD34 + /CD45 dim ) and HSC (CD34 + /CD45 dim /CD38 − ) enumeration were phycoerythrin (PE)‐conjugated CD34 (1:100, Invitrogen, Grand Island, NY, USA), PE‐Cy 5‐conjugated CD38 antibody (1:50, BD Biosciences, San Jose, CA, USA), and FITC‐conjugated CD45 (1:200, Invitrogen).…”

“…2011; Niemiro et al . 2017, ). The differential expression of CD34 has been shown to suggest differences in origin of either AT‐MSCs or BM‐MSCs, and quantification was performed as previously described (Traktuev et al .…”

“…2017) are collectively referred to as circulating progenitor cells (CPCs) (Niemiro et al . 2017, ). All CPCs circulate in peripheral blood in low quantities, and home to extramedullary tissues to participate in tissue repair and inflammation (Emmons et al .…”