Circulating progenitor cells are increased in newly diagnosed untreated hypertensive patients with arterial stiffening but normal carotid intima-media thickness

Mandraffino, Giuseppe; Sardo, M.A.; Riggio, Stefania; Loddo, Saverio; Imbalzano, Egidio; Alibrandi, Angela; Saitta, Carlo; Cinquegrani, Maurizio; Mormina, Enricomaria; Saitta, Antonino

doi:10.1038/hr.2011.56

Cited by 32 publications

(6 citation statements)

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“…During this process, they are exposed to shear stress generated by interstitial fluid flow and blood flow, and it has been reported that when cultured EPCs undergo shear stress in a flow‐loading device, their differentiation into mature endothelial cells accelerates significantly (Yamamoto et al, 2003). In recent evidence, other researchers showed that the levels of a different phenotype of EPCs were increased in newly diagnosed untreated patients with hypertension with arterial stiffening but normal carotid IMT; the EPC numbers correlated with gp91phox (a source of oxidative stress), a reactive oxygen species and fibrinogen, suggesting that the EPC number may be increased in early phases of atherosclerosis before the development of wall thickening to maintain an adequate number of EPCs in peripheral blood (Mandraffino et al, 2011). Based on this model, further studies will clarify if correlations exist among oxidative stress, cavernous intimal thickness, and EPC numbers in patients with AED or if this alteration (increased IMT) occurs first for different calibers of the vessel.…”

Section: Discussionmentioning

confidence: 99%

Arterial Erectile Dysfunction: Reliability of Penile Doppler Evaluation Integrated With Serum Concentrations of Late Endothelial Progenitor Cells and Endothelial Microparticles

Vignera

Vicari

Condorelli

et al. 2012

Journal of Andrology

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ABSTRACT:We recently showed the diagnostic value of a new immunophenotype of blood endothelial progenitor cells (EPC) (CD45 2 /CD34 + /CD144 + ) and endothelial microparticles (EMP) (CD45 2 /CD144+ /annexin V + ) in patients with arterial erectile dysfunction (AED), particularly in patients with associated late-onset hypogonadism and/or metabolic syndrome. In addition, we evaluated the effects of androgen replacement therapy, aerobic physical activity, and tadalafil administration on these markers. The aim of this study was to evaluate the serum concentrations of EPCs and EMPs in a large cohort of patients with AED according to severity of cavernous arterial insufficiency evaluated by penile Doppler. A total of 120 patients (aged 58.0 6 6.0 years) with AED were enrolled in this study. Patients were classified into 3 groups based on value of peak systolic velocity (PSV). Group A: 37 patients with PSV ,25 cm/s (severe arterial insufficiency); group B: 40 patients with PSV between 25 and 29 cm/s (moderate arterial insufficiency); group C: 43 patients with PSV between 30 and 34 cm/s (mild arterial insufficiency). Twenty patients (aged 60.0 6 3.0 years) with psychogenic erectile dysfunction (PED) represented the control group. EPC and EMP blood concentrations were evaluated by flow cytometry. Patients with AED had significantly higher blood pressure, triglyceride levels, homeostasis model assessment index of insulin resistance, cavernous artery acceleration time, and intima-media thickness than those with PED, whereas International Index of Erectile Function score, high-density lipoprotein cholesterol level, and cavernous artery PSV were lower than those in PED. Both EPC and EMP levels were significantly higher in patients with AED compared with patients with PED. Among 3 groups of patients with AED, there were no significant differences in metabolic parameters examined, but group A showed significantly higher values of cavernous artery acceleration time and intima-media thickness than group B and group C. Finally, group A showed serum concentrations of EPCs and EMPs significantly higher compared with other groups with AED. Patients with AED showed worse metabolic parameters, cavernous artery parameters, and EPC and EMP levels compared with patients with PED. Among patients with AED, those with PSV ,25 cm/s showed worse findings of endothelial dysfunction. This suggests that AED is an expression of endothelial damage and that this original immunophenotype of EPCs and EMPs may be considered a predictor of endothelial dysfunction in patients with AED. Finally, this study confirmed the reliability of penile Doppler evaluation integrated with these serum markers of endothelial dysfunction.

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Section: Discussionmentioning

confidence: 99%

Arterial Erectile Dysfunction: Reliability of Penile Doppler Evaluation Integrated With Serum Concentrations of Late Endothelial Progenitor Cells and Endothelial Microparticles

Vignera

Vicari

Condorelli

et al. 2012

Journal of Andrology

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“…For this study, we identified and counted circulating CD34+ cells in peripheral blood. The cells were analyzed for the expression of surface antigens with direct multi-color analysis using fluorescein isothiocyanate (FITC)-conjugated, and phycoerythrin (PE)-conjugated monoclonal antibodies (mAbs) by flow cytometry analysis, as reported elsewhere [ 33 ]. Staining and analysis were performed using the International Society of Hematotherapy and Graft Engineering (ISHAGE) sequential strategy [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Vitamin D Status in Rheumatoid Arthritis: Inflammation, Arterial Stiffness and Circulating Progenitor Cell Number

et al. 2015

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Background and AimsSuboptimal vitamin D status was recently acknowledged as an independent predictor of cardiovascular diseases and all-cause mortality in several clinical settings, and its serum levels are commonly reduced in Rheumatoid Arthritis (RA). Patients affected by RA present accelerated atherosclerosis and increased cardiovascular morbidity and mortality with respect to the general population. In RA, it has been reported an impairment of the number and the activity of circulating proangiogenic haematopoietic cells (PHCs), including CD34+, that may play a role in endothelial homeostasis. The purpose of the study is to investigate the association between vitamin D levels and PHCs, inflammatory markers, and arterial stiffening in patients with RA.Methods and ResultsCD34+ cells were isolated from 27 RA patients and 41 controls. Vitamin D levels, C-reactive protein (CRP), fibrinogen, pulse wave velocity (PWV), and carotid intima-media thickness (cIMT) were also evaluated. CD34+ count and vitamin D levels were lower in RA patients as compared to controls, while fibrinogen, CRP, PWV and cIMT were higher in RA patients. CD34+ cell number appeared to be associated with vitamin D levels, and negatively correlated to fibrinogen and early atherosclerosis markers (PWV and cIMT); vitamin D levels appear also to be inversely associated to fibrinogen.ConclusionsRA patients with moderate disease activity presented with low vitamin D levels, low CD34+ cell count, increased PWV and cIMT; we found that vitamin D deficiency is associated to CD34+ cell reduction in peripheral blood, and with fibrinogen levels. This suggests that vitamin D might contribute to endothelial homeostasis in patients with RA.

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“…In the very early stage of hypertension a significant increase in circulating progenitor cells is associated with reactive oxygen species and oxidative stress [73] . Vasa et al [19] , after studying patients with CAD concluded that the migratory capacity of EPCs was reduced in those patients with hypertension, although their total number did not change significantly.…”

Section: Epcs and Arterial Hypertensionmentioning

confidence: 99%

Circulating endothelial and progenitor cells: Evidence from acute and long-term exercise effects

Koutroumpi¹

2012

WJC

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Circulating bone-marrow-derived cells, named endothelial progenitor cells (EPCs), are capable of maintaining, generating, and replacing terminally differentiated cells within their own specific tissue as a consequence of physiological cell turnover or tissue damage due to injury. Endothelium maintenance and restoration of normal endothelial cell function is guaranteed by a complex physiological procedure in which EPCs play a significant role. Decreased number of peripheral blood EPCs has been associated with endothelial dysfunction and high cardiovascular risk. In this review, we initially report current knowledge with regard to the role of EPCs in healthy subjects and the clinical value of EPCs in different disease populations such as arterial hypertension, obstructive sleep-apnea syndrome, obesity, diabetes mellitus, peripheral arterial disease, coronary artery disease, pulmonary hypertension, and heart failure. Recent studies have introduced the novel concept that physical activity, either performed as a single exercise session or performed as part of an exercise training program, results in a significant increase of circulating EPCs. In the second part of this review we provide preliminary evidence from recent studies investigating the effects of acute and long-term exercise in healthy subjects and athletes as well as in disease populations.

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Circulating progenitor cells are increased in newly diagnosed untreated hypertensive patients with arterial stiffening but normal carotid intima-media thickness

Cited by 32 publications

References 43 publications

Arterial Erectile Dysfunction: Reliability of Penile Doppler Evaluation Integrated With Serum Concentrations of Late Endothelial Progenitor Cells and Endothelial Microparticles

Arterial Erectile Dysfunction: Reliability of Penile Doppler Evaluation Integrated With Serum Concentrations of Late Endothelial Progenitor Cells and Endothelial Microparticles

Vitamin D Status in Rheumatoid Arthritis: Inflammation, Arterial Stiffness and Circulating Progenitor Cell Number

Circulating endothelial and progenitor cells: Evidence from acute and long-term exercise effects

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