Circulating rotavirus G and P strains post rotavirus vaccination in Eastern Mediterranean Region

Almalki, Shaia Saleh R.

doi:10.15537/smj.2018.8.21394

Cited by 11 publications

(5 citation statements)

References 65 publications

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“…In Meghalaya state, 3 strains showed P[8] and 2 showed P[4] genotypes specificity, while, the G genotype was not be typable. These data are consistent with the studies from various countries including Saudi Arabia and Ghana, which indicate that genotype G1P[8] is the most commonly reported and severe diarrhea associated strain followed by G2P[4] and G9P[8] genotypes, respectively (Almalki, 2018;Damanka et al, 2019). In contrast, the proportion of G3 strains (P[8] and P[6]) was significantly higher with the P[8] as the dominant genotype (80.3%) followed by P[6] (14.6%) among patients admitted to the hospital with severe dehydrating diarrhea (Athiyyah et al, 2019) was observed in Indonesia.…”

Section: G and P Genotyping Of Rotavirusessupporting

confidence: 92%

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Raorane

Dubal

Ghatak

et al. 2020

Heliyon

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Introduction Rotavirus is the leading cause of diarrhoea in young children in India, responsible for an estimated 21357 mean numbers of deaths in 2010. Various genotypes of rotaviruses evolved due to mutational changes have been recognized. In this study, we determined the genotypes of rotaviruses involved in diarrhea in Goa and Meghalaya states of India. Methods The dsRNA of rotaviruses was extracted from stool samples and detected by Ribonucleic Acid-Polyacrylamide gel electrophoresis (RNA PAGE) and Reverse transcription-polymerase Chain Reaction (RT-PCR) targeting the partial VP7 gene. The full length VP7 and partial VP4 genes of rotavirus strains were amplified by RT-PCR followed by nucleotide sequencing. The RotaC classification tool was used to determine the genotypes. Results The positivity of rotavirus by PAGE and RT-PCR was observed to be 43.10% and 39.65% in Goa and 38% and 36% in Meghalaya, respectively. Though long electrophoretic profile was appeared to be the most predominant rotavirus type in circulation in these two states, 96% of long and 84.61% short electropherotype profiles could be detected by RT-PCR. The dsRNA of rotavirus extracted from 36 samples could be transcribed and amplified by beg9end9 primers for G genotyping, while, 41 by con3con2 primers for P genotyping. G1P[8] and G1P[6] genotypes were commonly circulated in Goa and G1P[8] and G1P[4] genotypes in Meghalaya. On nucleotide analysis, 6 samples from Goa showed G1 genotype specificity, while, 3 showed P[8] specificity indicating the G1P[8] rotavirus circulating in Goa. In Meghalaya state, 3 strains showed P[8] and 2 showed P[4] genotype specificity. The majority of the G and P genotypes were closely related to each other and G1 genotypes appeared in two separate clusters, while, P[8] and P[4] appeared in the respective clusters. Conclusion The circulation of G1P[8], G1P[6] genotypes in Goa and the presence of G1P[8] and G1P[4] genotypes in Meghalaya was observed.

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Section: G and P Genotyping Of Rotavirusessupporting

confidence: 92%

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Raorane

Dubal

Ghatak

et al. 2020

Heliyon

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“… 19 reported G1P[4] as the common genotype in sewage isolates in Nigeria. In addition, a previous study in Detroit, MI reported G1P[4] as the dominant circulating strain in children with severe diarrhea, 55 and a review by Almalki and colleagues 63 identified G1P[4] as the dominant strain in Yemen. The emergence of G1P[4] might have resulted from reassortment with G2P[4].…”

Section: Discussionmentioning

confidence: 99%

Human rotavirus VP4 and VP7 genetic diversity and detection of GII norovirus in Ibadan as Nigeria introduces rotavirus vaccine

et al. 2022

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Objective This cross-sectional study investigated the circulating strains of rotavirus and screened for noravirus in Ibadan, Nigeria as the country introduces the rotavirus vaccine into its national immunization program. Methods Sixty-five stool samples were collected from children younger than 5 years with clinically diagnosed diarrhea and screened for the presence of rotavirus and norovirus using RT-PCR. Rotavirus-positive samples were further analyzed to determine the G and P genotypes using semi-nested multiplex PCR. Results The rates of rotavirus and norovirus positivity were 30.8% and 10.8%, respectively, whereas the rate of rotavirus and norovirus mixed infection was 4.6%. G1 was the predominant VP7 genotype, followed by G2, G9, and G1G2G9, whereas the predominant VP4 genotype was P[4], followed by P[6], P[8], and P[9]. The mixed P types P[4]P[8] and P[4]P[6] were also detected. G1P[4] was the most common VP4 and VP7 combination, followed by G2P[4], G1[P6], G1P[8], G2P[6], G2P[9], G9P[6], G2G9P[4], G2P[4]P[6], G1P[4]P[8], G2G9P[8], G1G2G9P[8], and G1[non-typable] P[non-typable], which were detected in at least 5% of the samples. Four samples had a combination of non-typable G and P types. Conclusions It is essential to monitor the circulation of virus strains prior to and during the implementation of the immunization program.

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“…The highest similarity of G2P [4] Moroccan genotypes from vaccinated children with the Italian RVA/ Human-wt/ITA/BERG08/2012/G2P [4] detected in a 14-year old boy, gives interesting information on the circulation of strains in these particular Mediterranean countries during 2012-2014. This genotype is documented to be the major human genotype persisting in >5 years Italian adults [18], while, it has been the major serotype circulating in the post-vaccination era in Morocco (24.4% vs. 15%) or in other Eastern Mediterranean Region [8,19]. The high conservation of VP7 and VP4 genes between either circulating G2P [4] strains in the pre-and post-vaccination periods involve their stability over time.…”

Section: Discussionmentioning

confidence: 97%

Molecular characterization of G2P[4] and G9P[8] rotaviruses strains isolated from Moroccan children fully vaccinated with Rotarix™ between 2013 and 2014

Boulahyaoui

Amine

Melloul³

et al. 2019

Alexandria Journal of Medicine

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Background: Here, we describe the characterization of the VP7 and VP4 genes of rotavirus group A (RVA) strains isolated from Moroccan infants with acute gastroenteritis (AGE) after receiving the Rotarix. Methods: Stool samples were collected from seven infants suffering with AGE. Vesikari 20point was used while collecting the AGE information. RT-PCR followed by sequencing was employed to determine partial sequences from VP7 and VP4 encoding genes. RVA genotypes were determined using the RotaC online classification tool.The Neighbor-Joining phylogenetic tree was performed in the MEGA 6.06 software as well as the amino acid (aa) alignments of VP7 and VP8* antigenic epitopes. Results: Five children had gastroenteritis episodes defined as severe on the Vesikari scale. Analysis of VP7 gene sequences revealed five G2 and two G9 genotypes. These genotypes were associated with P[4] and P[8], respectively. Phylogenetic analysis demonstrated that G2 and G9 sequences grouped together with Italian strain belonging to lineage II and III respectively. Their P[4] sequences showed only 65-73% identity and grouped into two separate sub-clusters. The Moroccan P[8] isolates did not display this correlation and segregate to lineage III. The VP8* aa of P[8] when compared with that of the Rotarix vaccine, has a six aa differences. Conclusion: Although, this study does not reveal prevalence rates of RVA in infants after the vaccine introduction, it reports the detection and genetic characteristics of the G2P[4] and G9P[8] RVA types in vaccinated children in Morocco. The findings are suggestive of continuous surveillance program on rotavirus evolution in the vaccinated population.

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Circulating rotavirus G and P strains post rotavirus vaccination in Eastern Mediterranean Region

Cited by 11 publications

References 65 publications

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Genotypic determination of human group A rotaviruses from Goa and Meghalaya states, India

Human rotavirus VP4 and VP7 genetic diversity and detection of GII norovirus in Ibadan as Nigeria introduces rotavirus vaccine

Molecular characterization of G2P[4] and G9P[8] rotaviruses strains isolated from Moroccan children fully vaccinated with Rotarix™ between 2013 and 2014

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