Circulating Small Extracellular Vesicles Profiling and Thrombin Generation as Potential Markers of Thrombotic Risk in Glioma Patients

Melnichnikova, O. S.; Zhilenkova, Yu. I.; Сироткина, О. В.; Zolotova, E. A.; Пищулов, К. А.; Tastanbekov, M. M.; Paltsev, Artem; Симакова, М. А.

doi:10.3389/fcvm.2022.789937

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…В то же время связь между количеством циркулирующих везикул и риском ВТЭО у онкологических больных не установлена. Кроме того, у пациентов с опухолями глиальной природы ранее было показано превалирование микровезикул тромбоцитарного происхождения и их ассоциация с показателями теста генерации тромбина [41].…”

Section: пути распространения подопланинаunclassified

The role of podoplanin in tumor-associated thrombosis

Savelyeva,

Pishchulov

2023

jour

View full text Add to dashboard Cite

Venous thromboembolism (VTE) is highly consequential for patients with cancer, it is the second leading cause of death in these patients. The literature describes several mechanisms leading to these complications. One of these is the interaction of podoplanin (PDPN), which is the subject of this article, with its receptor on platelets CLEC-2 (C-type lectin-like type II transmembrane receptor). This interaction leads to platelet activation and promotes hematogenous metastasis and thrombosis associated with cancer. Podoplanin is expressed in gliomas, squamous cell carcinoma, osteosarcoma by cancer cells, and it is also normally expressed in podocytes and lymphatic endothelial cells, but not in vascular endothelium. Thrombus inflammation initiates ectopic expression of podoplanin in vascular endothelial cells, which promotes thrombosis. It has been shown that podoplanin expression correlates with the incidence of VTE, as well as with cancer metastasis. Violation of the PDPN-CLEC-2 interaction may become a new direction in the development of drugs for the prevention of thrombosis for patients with cancer.

show abstract

Section: пути распространения подопланинаunclassified

The role of podoplanin in tumor-associated thrombosis

Savelyeva,

Pishchulov

2023

jour

View full text Add to dashboard Cite

show abstract

“…Thrombin, a protease, triggers the activation of a group of receptors known as protease-activated receptors (PARs), which include PAR-1, PAR-3, and PAR-4. Literature has reported a significant elevation in the incidence of venous thrombosis among glioma patients ( 17 ), which is attributed to the release of thrombin outside the blood vessels due to an imbalance in coagulation/fibrinolysis in patients with varying degrees of malignancy ( 18 ). Glioma stem cells (GSCs) activate platelets in a plasma-independent manner by utilizing both endogenous and extrinsic coagulation cascade factors.…”

Section: Introductionmentioning

confidence: 99%

A new prognostic model for glioblastoma multiforme based on coagulation-related genes

Zhou,

Deng,

et al. 2023

Transl Cancer Res

View full text Add to dashboard Cite

Background Glioblastoma multiforme (GBM) is the most aggressive, common, and lethal type of primary brain tumor. Multiple cancers have been associated with abnormalities in the coagulation system that facilitate tumor invasion and metastasis. In GBM, the prognostic value and underlying mechanism of coagulation-related genes (CRGs) have not been explored. Methods RNA sequencing (RNA-seq) and clinical information on GBM were obtained from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA), respectively. Following the identification of differentially expressed CRGs (DECRGs) between GBM and control samples, the survival-related DECRGs were selected via univariate and multivariate Cox regression analyses to establish a prognostic signature. The prognostic performance and clinical utility of the prognostic signature were assessed by the Kaplan-Meier (KM) analysis and receiver operating characteristic (ROC) curve analysis, and a nomogram was constructed. The signature genes-related underlying mechanisms were analyzed according to gene set enrichment analysis (GSEA), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and single-cell analysis. Finally, the difference in immune cell infiltration, stromal score, immune score, and Estimation of Stromal and Immune cells in Malignant Tumor tissues using Expression data (ESTIMATE) score were compared between different risk groups. Results A 5-gene prognostic signature ( PLAUR, GP6, C5AR1, SERPINA5, F2RL2 ) was established for overall survival (OS) prediction of GBM patients. The predicted efficiency of the prognostic signature was confirmed in TGGA-GBM dataset and validated in the CGGA-GBM dataset, revealing that it could differentiate GBM patients from controls well, and high risk score was accompanied with poor prognosis. Moreover, biological process (BP) and signaling pathway analyses showed that signature genes were mainly enriched in the functions of blood coagulation and tumor invasion and metastasis. Moreover, high-risk patients exhibited higher levels of immune cell infiltration, stromal score, immune score, and ESTIMATE score than that of low-risk patients. Conclusions An analysis of coagulation-related prognostic signatures was conducted in this study, as well as how signature genes may affect GBM progress, providing information that might provide new ideas for the development of GBM-related molecular targeted therapies.

show abstract

Extracellular vesicles in venous thromboembolism and pulmonary hypertension

Zhang,

Hu,

Wang

et al. 2023

J Nanobiotechnol

View full text Add to dashboard Cite

Venous thromboembolism (VTE) is a multifactorial disease, and pulmonary hypertension (PH) is a serious condition characterized by pulmonary vascular remodeling leading with increased pulmonary vascular resistance, ultimately leading to right heart failure and death. Although VTE and PH have distinct primary etiologies, they share some pathophysiologic similarities such as dysfunctional vasculature and thrombosis. In both conditions there is solid evidence that EVs derived from a variety of cell types including platelets, monocytes, endothelial cells and smooth muscle cells contribute to vascular endothelial dysfunction, inflammation, thrombosis, cellular activation and communications. However, the roles and importance of EVs substantially differ between studies depending on experimental conditions and parent cell origins of EVs that modify the nature of their cargo. Numerous studies have confirmed that EVs contribute to the pathophysiology of VTE and PH and increased levels of various EVs in relation with the severity of VTE and PH, confirming its potential pathophysiological role and its utility as a biomarker of disease severity and as potential therapeutic targets. Graphical Abstract

show abstract

Circulating Small Extracellular Vesicles Profiling and Thrombin Generation as Potential Markers of Thrombotic Risk in Glioma Patients

Cited by 4 publications

References 27 publications

The role of podoplanin in tumor-associated thrombosis

The role of podoplanin in tumor-associated thrombosis

A new prognostic model for glioblastoma multiforme based on coagulation-related genes

Extracellular vesicles in venous thromboembolism and pulmonary hypertension

Contact Info

Product

Resources

About