Circulating Suppressor Cells in Aplastic Anemia

Górski, Andrzej; Rowińska, D; Skopińska, E; Orłowski, T

doi:10.1111/j.1423-0410.1979.tb04511.x

Cited by 16 publications

(1 citation statement)

References 9 publications

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“…In the majority of cases, molecular interactions were shared by T cells derived from bone marrow and peripheral blood (supplemental Figures 10 and 11), supporting the notion that hematopoietic destruction in AA is mediated by both circulating and marrowinfiltrating lymphocytes. 19,55 However, only bone marrow-derived CD8 1 T cells displayed enhanced expression of TGF-b and FASL, suggesting the existence of some unique interactions between bone marrow-derived T cells and HSPCs.…”

Section: No Of Molecular Interactions Between T Cells and Hspcsmentioning

confidence: 99%

Single-cell transcriptomics dissects hematopoietic cell destruction and T-cell engagement in aplastic anemia

Zhu

Wang

et al. 2021

Blood

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Aplastic anemia (AA) is a T cell-mediated autoimmune disorder of the hematopoietic system manifested by severe depletion of the hematopoietic stem and progenitor cells (HSPCs). Nonetheless our understanding of the complex relationship between HSPCs and T cells is still obscure, mainly limited by techniques and the sparsity of HSPCs in the context of bone marrow failure. Here we performed single-cell transcriptome analysis of residual HSPCs and T cells to identify the molecular players from AA patients. We observed that residual HSPCs in AA exhibited lineage-specific alterations in gene expression and transcriptional regulatory networks, indicating a selective disruption of distinct lineage-committed progenitor pools. In particular, HSPCs displayed frequently altered alternative splicing events and skewed patterns of polyadenylation in transcripts related to DNA damage and repair, suggesting a likely role in AA progression to myelodysplastic syndromes. We further identified cell-type-specific ligand-receptor interactions as potential mediators for ongoing HSPCs destruction by T cells. By tracking patients after immunosuppressive therapy (IST), we showed that hematopoiesis remission was incomplete accompanied by IST insensitive interactions between HSPCs and T cells as well as sustained abnormal transcription state. These data collectively constitute the transcriptomic landscape of disrupted hematopoiesis in AA at single-cell resolution, providing new insights into the molecular interactions of engaged T cells with residual HSPCs and render novel therapeutic opportunities for AA.

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Section: No Of Molecular Interactions Between T Cells and Hspcsmentioning

confidence: 99%

Single-cell transcriptomics dissects hematopoietic cell destruction and T-cell engagement in aplastic anemia

Zhu

Wang

et al. 2021

Blood

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Hepatitis C virus infection in acquired aplastic anemia

et al. 1998

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Hepatitis-associated aplastic anemia (HAAA) is an uncommon disorder that usually is not due to hepatitis A or B virus infection. Hepatitis C virus (HCV) seropositivity is infrequently observed in aplastic anemia (AA) patients who have not been extensively transfused. However, HCV seropositivity may not be detected until several weeks or months after viral infection and AA patients may exhibit defective humoral immunity. Therefore, we evaluated sera from AA patients for the presence of HCV viremia using a reverse transcriptase polymerase chain reaction (RT-PCR) based assay and several serologic assays for HCV antibodies. Serum samples from 90 AA patients who presented to the UCLA Medical Center between March 1984 and February 1990 were analyzed. Overall, 17 patients were found to have HCV viremia by RT-PCR assay, of whom 14 had a positive second-generation HCV enzyme immunoassay (EIA-2) and only 6 were EIA-1 reactive. The frequency of HCV viremia increased with the duration of time between diagnosis and sample procurement, and the number of blood products transfused prior to sampling (P = 0.026). No patient who received fewer than 20 U of blood products or who was sampled less than 20 days after diagnosis had a positive HCV RT-PCR result. Of four patients with hepatitis-associated AA (HAAA), one who was sampled 23 days after diagnosis had hepatitis C viremia and a reactive EIA-2 assay. Therefore, the high frequency of HCV viremia in this patient population is most likely due to transfusion with contaminated blood products prior to the introduction of routine blood donor screening for HCV.

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