Circulating tumor stem like cells in oral squamous cell carcinoma: An unresolved paradox

Patel, Shanaya; Shah, Kavan; Mirza, Sheefa; Shah, Kanisha; Rawal, Rakesh

doi:10.1016/j.oraloncology.2016.10.019

Cited by 29 publications

(26 citation statements)

References 28 publications

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“…These results were in accordance to the results found by Patel et al (101) who concluded that circulating tumor cells exhibit immense potential in enhancing diagnosis, prognosis and response to therapy in solid malignancies.…”

Section: Discussionsupporting

confidence: 93%

“…(97) The finding of high CD44sol levels in patients affected by cancer might be related to the amount of the extracellular fraction of the CD44 antigen released by stem cells that remain on the tumor mass. (60,101) In the present study, ELISA was used to evaluate the concentrations of CD44sol in patients with OSCC in relation to benign epithelial tumors patients and normal donors (healthy individuals). The results revealed a significant correlation at (P > 0.001) between serum CD44 concentration in patients with OSCC, patients with benign tumors patients and normal donors (healthy individuals).…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

Khamis

Fouad²,

Raslan³

et al. 2017

OSJ

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BackgroundHead and neck cancer is a major health problem. Recent studies on the pathobiology of oral squamous cell carcinoma (OSCC) have led to the discovery of a small population of cancer cells with a consistent behavior with the features of cancer stem cells (CSCs). CSCs are required and responsible for initiation, maintenance and recurrence of disease. Molecular markers are commonly used for the identification of CSCs. CD44 is the most reported CSC marker in OSCC.The aim of the study was to evaluate and correlate the expression of CD44 in different histopathological grades of OSCC, as well as to assess the diagnostic and prognostic value of soluble CD44 (CD44sol) in peripheral blood of patients.Materials and methodsFifteen patients with OSCC were included; biopsies were histologically evaluated using haematoxylin and eosin. Serial sections were immunohistochemically stained by monoclonal antibody to CD44. The intensity of immunostaining of CD44 was calculated. Enzyme-linked immunosorbent assay (ELISA) method was used to determine the concentration of CD44sol in the blood serum.ResultsAll grades of OSCC showed membranous immunosignaling of CD44. The well, moderately and poorly differentiated OSCC cases showed weak, moderate and intense positive membranous immunosignaling of CD44 respectively.CD44sol levels were significantly higher in OSCC patients than they were in control groups. Soluble CD44 serum levels were significantly higher in poorly differentiated than they were in moderately and well differentiated.ConclusionCSCs detection in fixed human tissue and CD44sol detection in peripheral blood using ELISA seemed to be a promising method and may have a diagnostic and prognostic value in management of OSCC.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

Khamis

Fouad²,

Raslan³

et al. 2017

OSJ

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“…Increased serum solCD44 level was seen in patient group in compare with control group but there was no significant difference; But Khamis et al, in their study, measured all standard isoforms and variants of solCD44, concluded that serum levels of CD44 in patients with OSCC were significantly higher than patients with benign epithelial tumor, as well as healthy subjects in control group (Khamis et al, 2017). High serum levels of CD44 in patients with OSCC could be related to the amount of extracellular contraction of the CD44 antigen released by the remaining stem cells in the tumor mass (Allegra et al, 2012, Patel et al, 2016.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Salivary and Serum Soluble CD44 Levels between Patients with Oral SCC and Healthy Controls

Seyedmajidi

Foroughi

et al. 2018

Asian Pac J Cancer Prev

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Background:The most common type of oral cancer is oral squamous cell carcinoma. If it is diagnosed in the early stages; the success of the treatment can be increased. It seems that ELISA-based techniques as a screening tool for society are the most cost-effective methods for early diagnosis. CD44 is a key marker for the detection of SCC stem cells. The aim of this study was to compare the level of soluble CD44 in saliva and serum between patients with oral SCC and healthy controls.Materials and Methods:Saliva and serum were collected from 20 patients with primary OSCC and 20 healthy persons as control group. The samples were evaluated by an ELISA test kit. Data were analyzed by SPSS software version 22, chi-square, ANOVA, T-test and Spearman correlation test.Results:The mean of soluble CD44 level in serum and saliva of the patient and control groups are 531.51±228.95 and 453.3±113.74 (for serum) and 48.53±59.02 and 17.76±39.14 (for saliva) respectively. There was no statistically significant difference in serum and saliva solCD44 level between the patient and control groups (P value = 0.182 and P value = 0.061 respectively). Also, there was no significant correlation between the solCD44 level in each patient and control group in serum (P value = 0.61) and in saliva (P value = 0.445).Conclusions:Determination of solCD44 level in saliva and serum can be a useful method for diagnosis the person’s involvement with cancer cells and the cancer in the early stages. But according to the controversial outcomes of past studies, larger and more accurate studies are needed in groups with more cases of oral cancer.

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“…Our previous findings demonstrated a critical role of CD44 + subpopulation in circulating tumor cells of OSCC patients and its association (particularly CD44v6) with locoregional aggressiveness, relapse, and therapeutic resistance . Hence, in this study, we sought to identify genetic and epigenetic modulators of CD44v using various bioinformatic‐based analysis.…”

Section: Discussionmentioning

confidence: 99%

“…22 Hence, in this study, we sought to iden- in salivary cfRNA with different subsites of OSCC is in concordance with our previous findings wherein a similar expression pattern was observed in primary tumor and circulating tumor stem-like cells of OSCC patients. 22 These findings not only prompt toward the possibility of buccal mucosa as a subsite having a more aggressive behavior that would be difficult to eliminate with the conventional chemotherapeutic regimen but also suggest that different anatomic subsites of OSCC might have different context-dependent roles and might function as autonomous entities. [25][26][27][28]…”

Section: Quantitative Gene Expression Of Serum and Salivary Markersmentioning

confidence: 99%

Uncovering the potential of CD44v/SYNE1/miR34a axis in salivary fluids of oral cancer patients

Shah

Patel

Modi

et al. 2018

J Oral Pathology Medicine

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Circulating tumor stem like cells in oral squamous cell carcinoma: An unresolved paradox

Cited by 29 publications

References 28 publications

Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

Diagnostic and prognostic value of cancer stem cell marker CD44 and soluble CD44 in the peripheral Blood of patients with oral Squamous cell carcinoma

Comparison of Salivary and Serum Soluble CD44 Levels between Patients with Oral SCC and Healthy Controls

Uncovering the potential of CD44v/SYNE1/miR34a axis in salivary fluids of oral cancer patients

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