Circulation of Giardia duodenalis in domestic and wild animals from Amazon region: A systematic review

Reis, Lisiane Lappe dos; Lima, Dayane Costa de Souza; Silva, Túllio Romão Ribeiro da; Braga, Francisco Carlos de Oliveira; Nava, Alessandra; Vicente, Ana Carolina Paulo

doi:10.1016/j.actatropica.2022.106708

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…Assemblage A was recently identified in humans, cats and non-human primates from Manaus, Amazonas State, (11) the same area were both sloths were found. Moreover, this genotype has been found in humans, domestic and wild animals from the Amazon Region, (12,21) as well as in cosmopolitan regions around the world. (7,22) Fig.…”

Section: Resultsmentioning

confidence: 98%

“…(7,8,9,10) The Amazon Region is hot spot of biodiversity in the world and, so far, in the Brazilian Amazon, only assemblages A and B were identified in humans, non-human primates and cats, and assemblage C in dogs. (11,12) Therefore, we have maintained a surveillance program of Giardia parasite identification particularly, considering wild animals from Brazilian Amazon Region. In this context, we reported the identification and characterisation of G. duodenalis assemblage A in two northern sloths (Bradypus tridactylus) by means of the beta giardin (BG), the caffeine-induced death protein-1 like protein (CID1) and mitotic control protein Dis3 (DIS3) genes analysis.…”

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confidence: 99%

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Zoonotic Giardia duodenalis assemblage A in northern sloth from Brazilian Amazon

dos Reis,

de Souza,

Braga

et al. 2023

Mem. Inst. Oswaldo Cruz

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BACKGROUNDThe parasite Giardia duodenalis infects a wide range of vertebrate hosts, including domestic and wild animals as well as humans. Giardia is genotyped into eight assemblages (A-H). Zoonotic assemblages A and B have already been identified in humans and wild and domestic animals (non-human primates and cats) from Brazilian Amazon and in the world. Due to its zoonotic/zooanthroponotic nature, surveillance initiatives and the definition of Giardia assemblages are important in order to characterise the epidemiological scenario and to implement further control measures.OBJECTIVES Determine assemblages of G. duodenalis in sloths from the Brazilian Amazon Region.METHODS Faecal parasitological examination of sloths from Amazonas State. Polymerase chain reaction (PCR) targeting the beta giardin (BG), and genes from multilocus sequence typing (MLST) scheme, amplicon sequencing and phylogenetic analysis.FINDINGS Here, we identified, by microscopy, Giardia in two northern sloths (Bradypus tridactylus). These two samples were submitted to molecular assays and it was revealed that both were infected by G. duodenalis assemblage A. Phylogenetic analysis showed that they belong to assemblage A within sequences from humans and wild and domestic animals.CONCLUSION Therefore, besides showing, by the first time, the current presence of this parasite in sloths, our findings reveals that this wild animal species would be part of the zoonotic/zooanthroponotic scenario of this parasite in the Brazilian Amazon.

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Section: Resultsmentioning

confidence: 98%

mentioning

confidence: 99%

Zoonotic Giardia duodenalis assemblage A in northern sloth from Brazilian Amazon

dos Reis,

de Souza,

Braga

et al. 2023

Mem. Inst. Oswaldo Cruz

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“…The Giardia genus is considered to have eight valid species, including Giardia microti , Giardia cricetidarum , Giardia muris , Giardia ardeae , Giardia psittaci , Giardia agilis , Giardia peramelis , and Giardia duodenalis . Of them, G. duodenalis (synonmy G. intestinalis and G. lamblia ) is the only species of Giardia that infects humans [ 6 , 7 , 8 , 9 ]. In early investigations, previously reported G. duodenalis were divided into eight assemblages/genotypes (A–H), in which A and B belonged to the zoonotic group [ 10 , 11 ], and C–H were considered as host-specific assemblages: C and D for canines, E for artiodactyls, F for felines, G for rodents, and H for marine mammals [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Characterization of Giardia duodenalis and Blastocystis spp. in Black Goats in Shanxi Province, North China: From a Public Health Perspective

Xiao,

Su,

Zhang

et al. 2024

Animals

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Blastocystis spp. and Giardia duodenalis are two prevalent zoonotic intestinal parasites that can cause severe diarrhea and intestinal diseases in humans and many animals. Black goat (Capra hircus) farming is increasingly important in China due to the remarkable adaptability, high reproductive performance, rapid growth rate, and significant economic value of black goats. A number of studies have indicated that black goats are the potential reservoir of multiple zoonotic protozoans in China; however, the prevalence and zoonotic status of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province is still unknown. Thus, a total of 1200 fecal samples of black goats were collected from several representative regions at different altitudes in Shanxi Province and were examined for the presence and genotypes of G. duodenallis and Blastocystis spp. by amplifying the beta-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) loci of G. duodenalis and SSU rRNA of Blastocystis spp. using PCR and sequence analysis methods, respectively. The overall prevalence of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province were 7.5% and 3.5%, respectively. Two assemblages (B and E) of G. duodenalis and four subtypes (ST5, ST10, ST14, and ST30) of Blastocystis spp. were identified, with assemblage E and ST10 as the prevalent genotype and subtype in black goats, respectively. One novel multilocus genotype (MLG) was identified in MLG-E and was designated as MLG-E12. For both G. duodenalis and Blastocystis spp., the prevalence was significantly related to the region and age groups (p < 0.05). This is the first report on the prevalence of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province. These results not only provide baseline data for the prevention and control of both parasites in black goats in Shanxi Province, but also enhance our understanding of the genetic composition and zoonotic potential of these two parasites.

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“…The Amazon region is hot spot of biodiversity in the world and, so far, in the Brazilian Amazon, only assemblages A and B were identi ed in humans as well animals (non-human primates and cats), and assemblage C in dogs (Dos Reis et al, 2021;Reis et al 2023). Therefore, we have maintained a surveillance program of Giardia parasite identi cation particularly, considering wild animals from Brazilian Amazon region.…”

Section: Introductionmentioning

confidence: 99%

Zoonotic Giardia duodenalis Assemblage A in Northern Sloth from Brazilian Amazon

Reis,

de Souza,

Braga

et al. 2023

Preprint

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The zoonotic protozoa parasite Giardia duodenalis infects a wide range of vertebrate hosts, including domestic and wild animals as well as humans. G.duodenalis is one of the most common intestinal parasite in humans and mammals worldwide. In humans, the disease is called giardiasis, with symptoms such as acute diarrhea which may progress to a chronic stage, but most infections remain asymptomatic. In children, giardiasis has a negative impact on their growth and cognitive development. Zoonotic assemblages A and B have already been identified in humans and wild and domestic animals (non-human primates and cats) from Brazilian Amazon. Here, in a screening focusing Giardia identification in wild animals from the Brazilian Amazon region, we identified, by microscopy, Giardia in two Northern sloths (Bradypus tridactylus). Samples from these two individuals were submitted to molecular assays, PCR targeting the beta giardin gene and amplicon sequencing, and it was revealed that both were infected by G. duodenalisfrom zoonotic assemblage A. In addition, the phylogenetic analysis showed that each one belong to a distinct assemblage A sub-cluster within sequences from humans and animals. Therefore, besides showing, by the first time, the presence of this parasite in sloths, our findings reveals that this wild animal species would be part of the zoonotic scenario of this parasite in the Brazilian Amazon.

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Circulation of Giardia duodenalis in domestic and wild animals from Amazon region: A systematic review

Cited by 8 publications

References 23 publications

Zoonotic Giardia duodenalis assemblage A in northern sloth from Brazilian Amazon

Zoonotic Giardia duodenalis assemblage A in northern sloth from Brazilian Amazon

Prevalence and Genetic Characterization of Giardia duodenalis and Blastocystis spp. in Black Goats in Shanxi Province, North China: From a Public Health Perspective

Zoonotic Giardia duodenalis Assemblage A in Northern Sloth from Brazilian Amazon

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