Circulation of human metapneumovirus among children with influenza‐like illness in Wuhan, China

Kong, Wenhua; Wang, Ying; Zhu, Honghao; Lin, Xiangui; Yu, Bin; Hu, Qinxue; Yang, Xiufen; Guo, Deyin; Peng, Jinsong; Zhou, Dun-Jin

doi:10.1002/jmv.24411

Cited by 15 publications

(21 citation statements)

References 33 publications

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“…The bias in the composition of our study population (nearly 50% of children under 5) is another factor that would have accentuated the trend noted. No influence of the patients’ gender on HMPV infection was observed in our study, which is consistent with the results of a study in China [25]. However, sex discrimination had been previously reported [26, 27].…”

Section: Discussionsupporting

confidence: 93%

“…However, sex discrimination had been previously reported [26, 27]. Our findings regarding Influenza, HAdV and HRV as the most frequently co-detected viruses with HMPV were similar to those of Kong et al [25]. Fever, cough, myalgia, and headache were the predominant clinical features associated with HMPV detection, which is in line with results from a study conducted in China [28].…”

Section: Discussionsupporting

confidence: 92%

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Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

et al. 2019

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Background Human metapneumovirus (HMPV) is a causal agent of acute respiratory infection, especially in primarily children. At the clinical level, HMPV is associated to several diseases including bronchitis, croup, pneumonia, bronchiolitis, reactive airway disease, chronic obstructive pulmonary disease and asthma exacerbations, specifically in children less than 5 years. Here, we carried out a retrospective pilot study, based on the processing of nasopharyngeal swabs, with a focus on the epidemiology and molecular characteristics of HMPV in Senegal. Methods This retrospective study was conducted from January 2012 to December 2016. Briefly, all outpatients presenting to healthcare sentinel sites were screened for surveillance enrollment and included if they met criteria for ILI. Naso-oropharyngeal swabs were collected from eligible participants. For viral respiratory pathogens detection, including HMPV, the Anyplex™ II RV16 Detection kit was used. A fragment of the hMPV F gene was targeted for sequencing. Results In total, 8209 patients with ILI were enrolled. Half of them (49.7%) were children under 5 years. Fever was the most common symptom followed by cough, and rhinitis. Three hundred eight patients were positive for HMPV (3.75%). 89 (28.9%) were detected as single infection. In co-infection cases, the most common co-infecting viruses were influenza, adenovirus and rhinovirus. HMPV detection rates in the different age groups varied significantly with the children under 5 years group accounting for 71.7% of positive patients. The temporal distribution pattern for HMPV infection showed a clear seasonal pattern with a higher activity during the rainy period (July–September). Phylogenetic analyses revealed that HMPV specimens circulating in Senegal were distributed into the two main genetic lineages, A and B. We also noted a co-circulation of both genetic lineages during the whole study period except in 2014. Conclusion In summary, the present study characterized the recent prevalence, seasonality and genetic diversity of HMPV in a large outpatient population presented with ILI in Senegal between 2012 and 2016. Globally our results show a clear seasonal circulation pattern of HMPV in Senegal. Our findings identified children less than 5 years as more susceptible group to HMPV infection. Molecular studies identified A2, B1 and B2 as the major genotypes circulating.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

et al. 2019

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“…Meanwhile, sub-lineages A1 and A2a were not detected in our study population. Similarly, sub-lineage A1 which circulated in China31, was also not detected in the neighbouring regions including Cambodia6, Thailand8 and Singapore4; and worldwide after 2006, in Austria13, Argentina32, Australia33, Canada12, and the United States3435. On the other hand, sub-lineage A2a circulated widely at a low prevalence in other countries including Thailand8 and Canada36.…”

Section: Discussionmentioning

confidence: 98%

Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes

Chow

Chan

Oong

et al. 2016

Sci Rep

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Human metapneumovirus (HMPV) is an important viral respiratory pathogen worldwide. Current knowledge regarding the genetic diversity, seasonality and transmission dynamics of HMPV among adults and children living in tropical climate remains limited. HMPV prevailed at 2.2% (n = 86/3,935) among individuals presented with acute respiratory tract infections in Kuala Lumpur, Malaysia between 2012 and 2014. Seasonal peaks were observed during the northeast monsoon season (November–April) and correlated with higher relative humidity and number of rainy days (P < 0.05). Phylogenetic analysis of the fusion and attachment genes identified the co-circulation of three known HMPV sub-lineages, A2b and B1 (30.2% each, 26/86) and B2 (20.9%, 18/86), with genotype shift from sub-lineage B1 to A2b observed in 2013. Interestingly, a previously unrecognized sub-lineage of A2 was identified in 18.6% (16/86) of the population. Using a custom script for network construction based on the TN93 pairwise genetic distance, we identified up to nine HMPV transmission clusters circulating as multiple sub-epidemics. Although no apparent major outbreak was observed, the increased frequency of transmission clusters (dyads) during seasonal peaks suggests the potential roles of transmission clusters in driving the spread of HMPV. Our findings provide essential information for therapeutic research, prevention strategies, and disease outbreak monitoring of HMPV.

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“…These observations were consistent with those made in Beijing, which indicated that A2, B1 and B2 co-circulated, and A2 was the most prevalent genotype from 2008 to 2010 37 . The most prevalent genotype was A in 2008-2009, which shifted to B in 2010-2011 and then shifted back to A in 2012-2013 in Wuhan 22 .…”

Section: Discussionmentioning

confidence: 96%

Experiments Investigating the Competitive Growth Advantage of Two Different Genotypes of Human Metapneumovirus: Implications for the Alternation of Genotype Prevalence

Zhou

Zhang

Cui

et al. 2020

Sci Rep

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Human metapneumovirus (hMPV) is an important pathogen that causes upper and lower respiratory tract infections in children worldwide. hMPV has two major genotypes, hMPV-A and hMPV-B.Epidemiological studies have shown that the two hMPV genotypes alternate in predominance worldwide in recent years. Co-circulation of the two genotypes of hMPV was usually observed and there is no study about the interaction between them, such as competitive replication, which maybe the possible mechanisms for alternating prevalence of subtypes. Our present study have used two different genotypes of hMPV (genotype A: NL/1/00; B: NL/1/99) in different proportions in animal model (BALB/c mice) and cell model (Vero-E6) separately. The result showed that the competitive growth does exist in BALB/c mice, genotype B had a strong competitive advantage. However, genotype B did not cause more severe disease than non-predominant (genotype A) or mixed strains in the study, which were evaluated by the body weight, airway hyperresponsiveness and lung pathology of mouse. In cell model, competitive growth and the two genotypes alternately prevalence were observed. In summary, we confirmed that there was a competitive replication between hMPV genotype A and B, and no difference in disease severity caused by the two subtypes. This study shows a new insight to understand the alternation of hMPV genotype prevalence through genotype competition and provide experimental evidence for disease control and vaccine design.Human metapneumovirus (hMPV), which was first isolated from children with respiratory tract infections (RTIs) in the Netherlands in 2001 and belongs to the Paramyxoviridae family and Pneumovirus subfamily 1 , is a leading cause of respiratory infections in children worldwide 2,3 . hMPV has been recognized as a causative agent of respiratory infections, including upper respiratory infections, severe bronchiolitis and pneumonia, in all age groups, with more severe disease occurring in infants, elderly people, and immunocompromised hosts 4-7 . The epidemic season of hMPV is reported to occur from winter to early spring 8 . There are no effective vaccines or drugs to combat hMPV infection.hMPV has been speculated to have similar characteristics as human respiratory syncytial virus (RSV), and this has been gradually confirmed by research over the last 18 years 8-10 . The seasonality, clinical manifestations, and age distribution of hMPV infection resemble those of RSV infection, and the alternating pattern of the two

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Circulation of human metapneumovirus among children with influenza‐like illness in Wuhan, China

Cited by 15 publications

References 33 publications

Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016

Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes

Experiments Investigating the Competitive Growth Advantage of Two Different Genotypes of Human Metapneumovirus: Implications for the Alternation of Genotype Prevalence

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