Circulation of oxytetracycline- and ciprofloxacin-resistant commensal Escherichia coli strains in broiler chickens and farm environments, Bangladesh

Das, Avijit; Dhar, Pangkaj Kumar; Dutta, Avijit; Jalal, Mohammad; Ghosh, P. K.; Das, Tridip; Barua, Himel; Biswas, Paritosh Kumar

doi:10.14202/vetworld.2020.2395-2400

Cited by 17 publications

(13 citation statements)

References 20 publications

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“…Antibiogram profiling of all 425 isolates showing maximum resistance against tetracycline is corroborative with the previous findings in Bangladesh ( 30 ), Iran ( 31 ), Malaysia ( 32 ), and Egypt ( 33 ). The resistance of poultry origin -Enterobacteriaceae to quinolone antibiotics (ciprofloxacin) from South China ( 34 ), Spain ( 35 ), and Egypt ( 36 ) was also reported, where co-resistance to ciprofloxacin and tigecycline was reported.…”

Section: Discussionsupporting

confidence: 88%

Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of β-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel β-lactamase variants

Bhowmick

Pal²,

Sunder³

et al. 2023

Front. Vet. Sci.

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ObjectivesThe present study was conducted to detect the occurrence of β-lactamase and biofilm-producing Escherichia coli, Salmonella, and Klebsiella in broilers and native fowl reared in the Andaman and Nicobar Islands, India. The study also included molecular docking experiments to confirm the nature of the catalytic domains found in the β-lactamase variants obtained and to reveal the clonal relationship of the isolates with human clinical strains from the database.Materials and methodsA total of 199 cloacal swabs were collected from five poultry breeds/varieties (broiler, Vanraja, Desi, Nicobari, and layer) in three districts of the Andaman and Nicobar Islands. E. coli, Salmonella enterica, and Klebsiella pneumoniae were isolated by standard techniques and confirmed by PCR. Phenotypical β-lactamase producers were identified by a double-disc test. The genes (blaCTX, blaSHV, blaTEM, and blaAmpC) were screened, and selected sequences of β-lactamase variants were submitted to DDBJ. Homology modeling, model validation, and active site identification of different β-lactamase variants were done by the SWISS-MODEL. Molecular docking was performed to identify the catalytic domains of the β-lactamase variants. The selected β-lactamase sequences were compared with the Indian ESBL sequences from human clinical strains in NCBI-GenBank.ResultsIn total, 425 Enterobacteriaceae strains were isolated from the collected samples. Klebsiella pneumoniae (42.58%) was found to be the most prevalent, followed by Salmonella enterica (30.82%) and E. coli (26.58%). The phenotypical antibiogram of all 425 isolates showed the highest resistance against oxytetracycline (61–76%) and the lowest against gentamicin (15–20%). Phenotypical production of β-lactamase enzymes was observed in 141 (33.38%) isolates. The isolation rate of β-lactamase producing E. coli, Salmonella enterica, and Klebsiella pneumoniae was significantly higher (p < 0.05) in the birds reared in the South Andaman district (25.6, 17.5, and 18.7%, respectively) than in Nicobar (11.5, 7.6, 7.1%, respectively). Genotyping of the β-lactamase-producing isolates revealed the maximum possession of blaTEM, followed by blaSHV and blaCTX − M. The nucleotide sequences were found to be similar with blaCTX − M−15, blaSHV − 11, blaSHV − 27, blaSHV − 228, blaTEM − 1, and blaAmpC in BLAST search. Distribution of studied biofilm-associated genes in Enterobacteriaceae strains from different varieties of the birds revealed that the layer birds had the maximum possession, followed by Vanraja, Desi, broilers, and Nicobari fowls. The phylogenetic analysis of selected sequences revealed a partial clonal relationship with human clinical strains of the Indian subcontinent. Molecular docking depicted the Gibbs free energy release for 10 different macromolecules (proteins) and ligand (antibiotic) complexes, ranging from −8.1 (SHV-27 + cefotaxime) to −7 (TEM-1 + cefotaxime) kcal/mol.Conclusion and relevanceThe study revealed β-lactamase variants circulating in the fowl population of the Andaman and Nicobar Islands (India), even in remote places with low anthropogenic activity. Most of the strains possessed blaTEM − 1, followed by blaCTX − M−15. Possession of blaSHV − 11, blaSHV − 27, and blaSHV − 228 in poultry Enterobacteriaceae strains was not reported earlier from any part of the world. The phylogenetic analysis revealed a partial clonal relationship of β-lactamase sequences with the human clinical strains isolated from the Indian subcontinent.

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Section: Discussionsupporting

confidence: 88%

Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of β-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel β-lactamase variants

Bhowmick

Pal²,

Sunder³

et al. 2023

Front. Vet. Sci.

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“…The detection of tetA gene in all the E. coli isolates exhibiting resistance to oxytetracycline suggests that tetA might be responsible for developing resistance to oxytetracycline where the contributions of other two genes, tetB, and tetC might be little. However, this result was almost similar to Das [ 27 ] who showed that 35% E. coli isolates harbored the tetA gene showing resistance to oxytetracycline. There were 16 reported tet genes.…”

Section: Author's Point Of Viewssupporting

confidence: 87%

Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated

Ferdous,

Ferdouse,

Hossain

et al. 2023

Biochemistry and Biophysics Reports

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“…E. coli isolates from apparently healthy broiler chicken were found 100% resistant to ampicillin and tetracycline followed by sulfamethoxazole–trimethoprim (95%) and nalidixic acid (92%) [ 39 , 42 ]. The commonly detected oxytetracycline resistant genes were tetA, tetB and tetC gene [ 38 ]. Another study reported 55% prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-producing E. coli in broiler ceca and feces at households, farms, and live poultry markets.…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Usage and Resistance in Food Animal Production: What Have We Learned from Bangladesh?

et al. 2021

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Irrational and inappropriate use of antibiotics in commercial chicken and aquaculture industries can accelerate the antibiotic resistance process in humans and animals. In Bangladesh, the growing commercial chicken and aquaculture industries are playing significantly important roles in the food value chain. It is necessary to know the antibiotic usage practices and antibiotic resistance in food animal production to design rational policies, guidelines, and interventions. We conducted a narrative review to understand the level of antibiotic usage and resistance in food animal production in Bangladesh. Information about antibiotic usage in different food animal production systems, including commercial chickens and aquaculture in Bangladesh is inadequate. Only a few small-scale studies reported that the majority (up to 100%) of the broiler and layer chicken farms used antibiotics for treating and preventing diseases. However, numerous studies reported antibiotic-resistant bacteria of public health importance in commercial chicken, fish, livestock, and animal origin food. The isolates from different pathogenic bacteria were found resistant against multiple antibiotics, including quinolones, the third or fourth generation of cephalosporins, and polymyxins. Veterinary practitioners empirically treat animals with antibiotics based on presumptive diagnosis due to inadequate microbial diagnostic facilities in Bangladesh. Intensive training is helpful to raise awareness among farmers, feed dealers, and drug sellers on good farming practices, standard biosecurity practices, personal hygiene, and the prudent use of antibiotics. Urgently, the Government of Bangladesh should develop and implement necessary guidelines to mitigate irrational use of antibiotics in food animals using a multi-sectoral One Health approach.

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Circulation of oxytetracycline- and ciprofloxacin-resistant commensal Escherichia coli strains in broiler chickens and farm environments, Bangladesh

Cited by 17 publications

References 20 publications

Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of β-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel β-lactamase variants

Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of β-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel β-lactamase variants

Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated

Antibiotic Usage and Resistance in Food Animal Production: What Have We Learned from Bangladesh?

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