Circulation of Tc Ia discrete type unit Trypanosoma cruzi in Yucatan Mexico

Monteón, Vı́ctor; Triana-Chávez, Omar; Mejía-Jaramillo, Ana María; Pennignton, Pamela; Ramos-Ligonio, Ángel; Acosta, Karla; López, Ruth

doi:10.1007/s12639-014-0499-2

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…cruzi genotyping, DTUs reported for T . dimidiata are TcI (TcIa, TcId), TcII, TcV and TcVI [ 7 , 9 , 15 – 17 ]. This species feeds on many hosts, including sylvatic, domestic species, and humans [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Different infection rates with T. cruzi have been reported in Mexico, Guatemala, Ecuador, Costa Rica, Belize, and Colombia, ranging between 13.7-60% [5][6][7][8][9][10][11][12][13][14]. Regarding T. cruzi genotyping, DTUs reported for T. dimidiata are TcI (TcIa, TcId), TcII, TcV and TcVI [7,9,[15][16][17]. This species feeds on many hosts, including sylvatic, domestic species, and humans [17].…”

Section: Introductionmentioning

confidence: 99%

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Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia

et al. 2022

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Chagas disease is considered a public health issue in Colombia, where many regions are endemic. Triatoma dimidiata is an important vector after Rhodnius prolixus, and it is gaining importance in Boyacá, eastern Colombia. Following the recent elimination of R. prolixus in the region, it is pivotal to understand the behavior of T. dimidiata and the transmission dynamics of T. cruzi. We used qPCR and Next Generation Sequencing (NGS) to evaluate T. cruzi infection, parasite load, feeding profiles, and T. cruzi genotyping for T. dimidiata specimens collected in nine municipalities in Boyacá and explored T. dimidiata population genetics. We found that T. dimidiata populations are composed by a single population with similar genetic characteristics that present infection rates up to 70%, high parasite loads up to 1.46 × 109 parasite-equivalents/mL, a feeding behavior that comprises at least 17 domestic, synanthropic and sylvatic species, and a wide diversity of TcI genotypes even within a single specimen. These results imply that T. dimidiata behavior is similar to other successful vectors, having a wide variety of blood sources and contributing to the circulation of different genotypes of the parasite, highlighting its importance for T. cruzi transmission and risk for humans. In the light of the elimination of R. prolixus in Boyacá and the results we found, we suggest that T. dimidiata should become a new target for vector control programs. We hope this study provides enough information to enhance surveillance programs and a future effective interruption of T. cruzi vector transmission in endemic regions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia

et al. 2022

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“…The TcI genotype is widely distributed in northern South America, Central America and Mexico, and is responsible for the majority of the clinical manifestations of Chagas disease in Mexican patients (Monteon et al ., 2016). Mexican strains of T. cruzi I (TcIa) have distinct biological characteristics such as metacyclogenesis, growth, infectivity and they also differ in their ability to invade cells and cause infection (Gómez-Hernández et al ., 2011, 2019; Barbosa et al ., 2019 a , 2019 b ).…”

Section: Introductionmentioning

confidence: 99%

Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

et al. 2021

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Congenital transmission of Chagas disease plays an important role in endemic countries because it is not a diagnosis that is encountered frequently in prenatal care. Due to limited information regarding congenital transmission of Trypanosoma cruzi in Mexico, the present study aimed to investigate protozoan infectivity and modulation of immune responses in human placental explants infected with T. cruzi Ia Mexican strains. The Inc-5 strain showed increased infectivity and modulated IL-1β, IL-10 and TLR-4, decreasing their expression after 24 h of infection. Both strains (Inc-5 and Ninoa) stimulated the production of TNF-α and decreased IL-6 levels 96 h after infection. An important detachment of the syncytiotrophoblast caused by infection with T. cruzi was observed after 24 h of infection. In this study, ex vivo infection of human placental villi was performed to better understand interactions involving parasitic T. cruzi and human placental tissue. It was concluded that the strains of TcIa present parasitism in placental tissue, modulation of the innate immune system of the placenta, and cause intense detachment of the syncytiotrophoblast, a fact that may be more associated with abortion and premature birth events than the congenital transmission itself, justifying the low rate of this transmission mechanism by this genotype.

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“…Given that historical studies [ 21 , 22 , 26 ], and the findings of the present work, confirmed that TcI is the only genotype detected in Mexico from human isolates from various states of the Mexican Republic, and that there is no genetic variability in any of them, it is possible to propose the development of in silico strategies for the production of antigens to optimise the diagnosis of Chagas disease such as quantitative ELISA methods that use this region of Tc24 without the need for parasitic cultures, and with the possibility of reducing biosafety requirements in diagnostic laboratories. For this reason, the invariance of the Tc24 fragment present in the only DTU (TcI) isolated from human patients in Mexico may offer hope for the production of better diagnostic systems and the potential development of a vaccine.…”

Section: Discussionmentioning

confidence: 99%

The Low Variability of Tc24 in Trypanosoma cruzi TcI as an Advantage for Chagas Disease Prophylaxis and Diagnosis in Mexico

et al. 2023

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(1) Background: Chagas disease is the main neglected tropical disease in America. It is estimated that around 6 million people are currently infected with the parasite in Latin America, and 25 million live in endemic areas with active transmission. The disease causes an estimated economic loss of USD 24 billion dollars annually, with a loss of 75,200 working years per year of life; it is responsible for around ~12,000 deaths annually. Although Mexico is an endemic country that recorded 10,186 new cases of Chagas disease during the period of 1990–2017, few studies have evaluated the genetic diversity of genes that could be involved in the prophylaxis and/or diagnosis of the parasite. One of the possible candidates proposed as a vaccine target is the 24 kDa trypomastigote excretory–secretory protein, Tc24, whose protection is linked to the stimulation of T. cruzi-specific CD8+ immune responses. (2) Methods: The aim of the present study was to evaluate the fine-scale genetic diversity and structure of Tc24 in T. cruzi isolates from Mexico, and to compare them with other populations reported in the Americas with the aim to reconsider the potential role of Tc24 as a key candidate for the prophylaxis and improvement of the diagnosis of Chagas disease in Mexico. (3) Results: Of the 25 Mexican isolates analysed, 48% (12) were recovered from humans and 24% (6) recovered from Triatoma barberi and Triatoma dimidiata. Phylogenetic inferences revealed a polytomy in the T. cruzi clade with two defined subgroups, one formed by all sequences of the DTU I and the other formed by DTU II–VI; both subgroups had high branch support. Genetic population analysis detected a single (monomorphic) haplotype of TcI throughout the entire distribution across both Mexico and South America. This information was supported by Nei’s pairwise distances, where the sequences of TcI showed no genetic differences. (4) Conclusions: Given that both previous studies and the findings of the present work confirmed that TcI is the only genotype detected from human isolates obtained from various states of Mexico, and that there is no significant genetic variability in any of them, it is possible to propose the development of in silico strategies for the production of antigens that optimise the diagnosis of Chagas disease, such as quantitative ELISA methods that use this region of Tc24.

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Circulation of Tc Ia discrete type unit Trypanosoma cruzi in Yucatan Mexico

Cited by 11 publications

References 16 publications

Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia

Estimating the genetic structure of Triatoma dimidiata (Hemiptera: Reduviidae) and the transmission dynamics of Trypanosoma cruzi in Boyacá, eastern Colombia

Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

The Low Variability of Tc24 in Trypanosoma cruzi TcI as an Advantage for Chagas Disease Prophylaxis and Diagnosis in Mexico

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