Circulatory miRNA-484, 524, 615 and 628 expression profiling in HCV mediated HCC among Egyptian patients; implications for diagnosis and staging of hepatic cirrhosis and fibrosis

El-Maraghy, Shohda A.; Adel, Ola; Yosry, Ayman; El-Nahaas, Saeed M; Gibriel, Abdullah Ahmed

doi:10.1016/j.jare.2019.12.002

Cited by 38 publications

(28 citation statements)

References 30 publications

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“…In metastatic HCC tissues and cell lines [64] and in non-metastatic conditions [31], the IGF2/IGFR1/mTOR axis is overexpressed, which in turn correlates to the low level of miR-615-5p. The low expression was also recently documented in plasma from HCC patients [28]. This evidence is consistent with its negative role in cell proliferation, invasiveness and migration [31].…”

Section: Mir-615-5p As a Tumor Suppressor In Several Types Of Cancersupporting

confidence: 78%

“…On the other hand, the involvement of miR-615-5p in the reparative processes and angiogenesis has been demonstrated in various contexts. In human liver tissue, miR-615-5p is found to be moderately upregulated in cirrhotic liver [27], as well as in plasma from patients with different degrees of liver fibrosis [28]. Therefore, the authors suggested that it may be relevant as a biomarker in the liver repair process.…”

Section: Mir-615 Targets Under Physiological and Pathological Conditionsmentioning

confidence: 99%

“…It does so mainly by preventing invasiveness and metastasis, the main causes of tumor reappearance. With the exception of some examples of HCC [27,51], a downregulation of this miRNA has been documented in several malignancies of different lineages such as carcinomas [28,52,53], lymphomas [54] and glioblastomas [55], with an indirect relationship between the level of expression of miR-615-5p and the tumor cell capacity for proliferation, invasion, migration and angiogenesis. Their target genes are summarized in Table 2 and Figure 2.…”

Section: Mir-615-5p As a Tumor Suppressor In Several Types Of Cancermentioning

confidence: 99%

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miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair

Godínez-Rubí

Ortuño-Sahagún

2020

Cells

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MicroRNAs (miRNAs) are small noncoding RNAs that function as epigenetic modulators regulating almost any gene expression. Similarly, other noncoding RNAs, as well as epigenetic modifications, can regulate miRNAs. This reciprocal interaction forms a miRNA-epigenetic feedback loop, the deregulation of which affects physiological processes and contributes to a great diversity of diseases. In the present review, we focus on miR-615, a miRNA highly conserved across eutherian mammals. It is involved not only during embryogenesis in the regulation of growth and development, for instance during osteogenesis and angiogenesis, but also in the regulation of cell growth and the proliferation and migration of cells, acting as a tumor suppressor or tumor promoter. It therefore serves as a biomarker for several types of cancer, and recently has also been found to be involved in reparative processes and neural repair. In addition, we present the pleiad of functions in which miR-615 is involved, as well as their multiple target genes and the multiple regulatory molecules involved in its own expression. We do this by introducing in a comprehensible way the reported knowledge of their actions and interactions and proposing an integral view of its regulatory mechanisms.

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Section: Mir-615-5p As a Tumor Suppressor In Several Types Of Cancersupporting

confidence: 78%

Section: Mir-615 Targets Under Physiological and Pathological Conditionsmentioning

confidence: 99%

Section: Mir-615-5p As a Tumor Suppressor In Several Types Of Cancermentioning

confidence: 99%

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miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair

Godínez-Rubí

Ortuño-Sahagún

2020

Cells

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“…It occurs more frequently among men than women, with increasing incidence rates in almost all countries, particularly in East Asia [29]. The etiologies of HCC include metabolic diseases [30], alcohol-related cirrhosis [31], hepatitis B virus (HBV) [32], and hepatitis C virus (HCV) [33]. HCV-associated hepatitis develops into HCC via a myc-unregulated mechanism [34], whereas non-alcoholic steatohepatitis-dependent disease is not involved in the expression of myc [35].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potentially Therapeutic Target Genes of Hepatocellular Carcinoma

2020

IJERPH

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Background: Hepatocellular carcinoma (HCC) is a major threat to public health. However, few effective therapeutic strategies exist. We aimed to identify potentially therapeutic target genes of HCC by analyzing three gene expression profiles. Methods: The gene expression profiles were analyzed with GEO2R, an interactive web tool for gene differential expression analysis, to identify common differentially expressed genes (DEGs). Functional enrichment analyses were then conducted followed by a protein-protein interaction (PPI) network construction with the common DEGs. The PPI network was employed to identify hub genes, and the expression level of the hub genes was validated via data mining the Oncomine database. Survival analysis was carried out to assess the prognosis of hub genes in HCC patients. Results: A total of 51 common up-regulated DEGs and 201 down-regulated DEGs were obtained after gene differential expression analysis of the profiles. Functional enrichment analyses indicated that these common DEGs are linked to a series of cancer events. We finally identified 10 hub genes, six of which (OIP5, ASPM, NUSAP1, UBE2C, CCNA2, and KIF20A) are reported as novel HCC hub genes. Data mining the Oncomine database validated that the hub genes have a significant high level of expression in HCC samples compared normal samples (t-test, p < 0.05). Survival analysis indicated that overexpression of the hub genes is associated with a significant reduction (p < 0.05) in survival time in HCC patients. Conclusions: We identified six novel HCC hub genes that might be therapeutic targets for the development of drugs for some HCC patients.

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“…19 Various miRNAs will impact progress of HCC and OS in patients with HCC to different degrees. [20][21][22][23][24][25] Therefore, it is reasonable to assume that expression of miRNAs could provide valuable prognostic references for patients with HCC.…”

Section: Introductionmentioning

confidence: 99%

Construction of a 13‐microRNA‐based signature and prognostic nomogram for predicting overall survival in patients with hepatocellular carcinoma

Zheng

Wen

Nie

et al. 2020

Hepatology Research

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Aim: Hepatocellular carcinoma (HCC) is a common malignancy associated with a poor prognosis due to difficulties in reliably estimating overall survival (OS). MicroRNAs (miRNAs) play critical roles in HCC initiation, progression, and metastasis and are highly correlated with patient prognosis. Thus, miRNA based risk signatures and nomograms are urgently required for predicting OS in patients with HCC. Methods: We constructed a 13 miRNA based signature and prognostic nomogram using 408 HCC samples and 58 normal tissues with miRNA sequencing data and clinical data from 323 patients downloaded from The Cancer Genome Atlas. A total of 195 patients were assigned as the internal validation cohort for verification and testing. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis was applied to investigate pathway enrichment for the signature. Results: We identified and validated a 13 miRNA risk signature highly associating with the OS of HCC patients. The signature showed good performances by calculating C index, area under the curve, and calibration curves. After verification and testing using an internal validation cohort, the results yielded a miRNA based signature and a prognostic nomogram with reliable predictive accuracy. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that various genes and multiple pathways were closely related to the mechanisms of HCC proliferation and metastasis. Conclusion: We successfully identified a 13 miRNA based signature and prognostic nomogram that are capable of predicting OS in patients with HCC.

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Circulatory miRNA-484, 524, 615 and 628 expression profiling in HCV mediated HCC among Egyptian patients; implications for diagnosis and staging of hepatic cirrhosis and fibrosis

Cited by 38 publications

References 30 publications

miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair

miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair

Identification of Potentially Therapeutic Target Genes of Hepatocellular Carcinoma

Construction of a 13‐microRNA‐based signature and prognostic nomogram for predicting overall survival in patients with hepatocellular carcinoma

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