CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell

Tong, Hui; Zhao, Kai; Wang, Jiang-Jie; Xu, Hui; Xiao, Jiangnan

doi:10.1111/jphp.13188

Cited by 36 publications

(16 citation statements)

References 29 publications

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“…10 For example, circZNF609 sequestered miR-134-5p to upregulating BTG-2 to elevate proliferation of glioma cells. 22 CircZNF609 enhanced gastric cancer migration and invasion through modulating CDK6 via sponging miR-483-3p. 23 In this study, we predicted miRNA targets of circZNF609 by CircBank informatic tool.…”

Section: Dovepressmentioning

confidence: 96%

<p>Circular RNA ZNF609 Promoted Hepatocellular Carcinoma Progression by Upregulating PAP2C Expression via Sponging miR-342-3p</p>

Liao

Zhan

Tian

et al. 2020

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Background: Emerging evidence has revealed that circular RNAs (circRNAs) participated in hepatocellular carcinoma (HCC) development. However, the roles of most circRNAs have not been studied. Methods: CircZNF609, miR-342-3p and RAP2C expressions were assessed by qPCR or Western blot. Loss-of-function experiments were performed using si-circZNF609 transfection, followed by CCK-8 assay, flow cytometry, wound healing assay and transwell assay. Informatic tools and rescue experiments were carried out to investigate the underlying mechanisms. Results: We showed that circZNF609 was overexpressed in HCC tissues and cells, as well as associated with poor clinical characteristics. Depletion of circZNF609 restrained HCC cell viability, migration and invasion while enhanced cell apoptosis. As to mechanism, miR-342-3p was sponged by circZNF609, and RAP2C was targeted by miR-342-3p. The effects on HCC cells induced by si-circZNF609 could be reversed by miR-342-3p inhibitor or RAP2C. In vivo, circZNF609 knockdown inhibited tumorigenesis of HCC mice, confirming the findings in vitro. Conclusion: CircZNF609 was highly expressed in HCC tissues and driven HCC progression by sponging miR-342-3p and upregulating RAP2C. This study may provide new potential therapeutic targets for HCC treatment.

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Section: Dovepressmentioning

confidence: 96%

<p>Circular RNA ZNF609 Promoted Hepatocellular Carcinoma Progression by Upregulating PAP2C Expression via Sponging miR-342-3p</p>

Liao

Zhan

Tian

et al. 2020

OTT

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“…CircRNAs are reported to closely associate with the progression of a large number of cancers [5]. Aberrant regulation of circRNAs has been noted in gliomas [6], renal cell carcinomas [7] and other human malignancies [8]. With the development of high-throughput sequencing and bioinformatics analysis, increasing numbers of novel circRNAs have been identified to be potential cancer prognostic biomarkers [9].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

et al. 2020

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Objective: Non-small cell lung cancer (NSCLC) continues to top the list of cancer mortalities worldwide. Early diagnosis and therapeutic interventions targeting NSCLC is becoming the world's significant challenge. Circular RNAs (circRNAs) are emerging as a group of potential cancer biomarkers. Materials and methods: Quantitative real-time PCR (qRT-PCR) was employed to examine the expression of circ 0072309 in NSCLC tissues and cell lines. Cell counting kit 8 (CCK-8), wound healing and Transwell assays were used to analyze cell proliferation, migration and invasion in A549 and H1299 cells. The relationship between circ 0072309 and miR-580-3 was analyzed by Luciferase reporter and RNA pull down assays. Results: We screened circ 0072309 from Gene Expression Omnibus and found that circ 0072309 was lowly expressed in NSCLC tissues and cell lines. The transfection of circ 0072309-overexpressing vector significantly suppressed the cell proliferation, migration and invasion in A549 and H1299 cells. We predicted that miR-580-3p is a target of circ 0072309 by using publicly available bioinformatic algorithms Circinteractome tool and confirmed that circ 0072309 directly bound to miR-580-3p. Furthermore, the addition of miR-580-3p mitigated the blockage of cell proliferation, migration and invasion induced by circ 0072309. Conclusions: These data showed that circ 0072309 inhibits the progression of NSCLC progression via blocking the expression of miR-580-3p. These findings revealed the anti-tumor role of circ 0072309 during the development of NSCLC and provided a novel diagnostic biomarker and potential therapy for NSCLC.

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“…MiR-452-5p exerted its function by negatively regulating KAT6B . Mounting data have indicated that circRNAs regulated the miRNA-mRNA axis to affect tumor occurrence and progression in multiple cancers [ 29 , 30 ], certainly including GC [ 31 , 32 ]. In our study, circKIAA0907 had a similar positive regulation on KAT6B expression as a miR-452-5p sponge in GC cells, and KAT6B downregulation could restore the effects of circKIAA0907 on GC cells.…”

Section: Discussionmentioning

confidence: 99%

CircKIAA0907 Retards Cell Growth, Cell Cycle, and Autophagy of Gastric Cancer In Vitro and Inhibits Tumorigenesis In Vivo via the miR-452-5p/KAT6B Axis

et al. 2020

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Background The significant roles of circular ribonucleic acids (RNAs) in cancers have been discussed in many studies. This report aimed to investigate the biological functions of circKIAA0907 and its action mechanism in gastric cancer (GC). Material/Methods Relative RNA expression levels were determined using quantitative real-time polymerase chain reaction (qRT-PCR). The examination of cell proliferation was performed via 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide assay. Flow cytometry was used to analyze the apoptosis rate and cell cycle. Protein levels were quantified using western blot. Biotinylated RNA pull-down assay was used to find the microRNA target of circKIAA0907; target binding was validated through dual-luciferase reporter assay. The assay in vivo was executed via a xenograft tumor model to explore the role of circKIAA0907 in GC. Results CircKIAA0907 was downregulated in GC and had higher stability than its linear isoform. Functionally, circKIAA0907 upregulation resulted in the repression of proliferation, cell cycle, and autophagy and promotion of apoptosis in GC cells. Mechanistically, circKIAA0907 bound to miR-452-5p as a specific sponge for it; lysine acetyltransferase 6B ( KAT6B ) was a target gene of miR-452-5p, so circKIAA0907 elevated KAT6B levels via sponging miR-452-5p. Reversion assays indicated that circKIAA0907 served as a tumor inhibitor by inhibiting miR-452-5p and increasing KAT6B; miR-452-5p inhibition impeded GC development by upregulating KAT6B. The miR-452-5p/KAT6B axis was also accountable for circKIAA0907-induced tumorigenesis suppression in vivo . Conclusions This work demonstrated that circKIAA0907 has diagnostic and therapeutic value in GC by acting as an oncogenic molecule via the miR-452-5p/KAT6B axis.

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CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell

Cited by 36 publications

References 29 publications

<p>Circular RNA ZNF609 Promoted Hepatocellular Carcinoma Progression by Upregulating PAP2C Expression via Sponging miR-342-3p</p>

<p>Circular RNA ZNF609 Promoted Hepatocellular Carcinoma Progression by Upregulating PAP2C Expression via Sponging miR-342-3p</p>

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

CircKIAA0907 Retards Cell Growth, Cell Cycle, and Autophagy of Gastric Cancer In Vitro and Inhibits Tumorigenesis In Vivo via the miR-452-5p/KAT6B Axis

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