Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid gastric administration during the pregestational and gestational periods does not influence the follicular endowment of the progeny

Abstract

Fetal programming suggests that maternal stimulation and nutrition during the period of fetal development can program the progeny. Conjugated linoleic acid (CLA), an isomer of linoleic acid, has been characterized in several aspects, but few studies have been performed on its involvement in reproduction and fetal programming. The aim of this study was to evaluate the F1, F2 and F3 progeny of female mice supplemented with CLA during the pregestational and gestational periods with respect to biometric and reproductive parameters, as well as ovarian morphophysiology. The F1 progeny of mothers supplemented with CLA exhibited stable weight gain, while the F2 progeny showed no effects (P=0.0187 and P=0.0245, respectively). A reduction in Lee's Index was observed in both generations at the second post-weaning evaluation week in the animals treated with CLA (P=0.0100 and P=0.0078, respectively). The F2 generation showed an increase in the anogenital index in both sexes of the animals treated with CLA (P= 0.0114 and P<0.0001, female and male respectively). CLA administration to mothers did not affect any of the following in their progeny: ovarian follicle mobilization (P>0.05), follicle number (P>0.05) and the integrated density of the lipid content of oocytes included in antral follicles (P>0.05). This study evaluated the use of CLA in mothers and found that it did not affect the progeny regarding murine reproductive performance, suggesting that this supplement can be used safely.

Trans 10, cis 12-conjugated linoleic acid reduced reproductive ability by disrupting the estrus cycle in female mice

“…Several studies have been carried out to investigate the effects of CLA on male and female reproductive and metabolic aspects and demonstrated that a mixture of CLA isomers provides some benefit on reproductive parameters ( Freitas et al, 2023 ; Safari Hasanabad et al, 2022 ). In contrast, it was difficult to determine whether this was the long-term effect of pure t10c12-CLA.…”

“…CLA could also affect female mice’s reproductive performance by reducing FSH and LH levels in the blood ( Badawy et al, 2023 ). While gastric administration of CLA mixtures during the pregestational and gestational periods did not affect the offspring’s ovarian follicle endowment and mobilization, nor did it affect oocyte lipid accumulation, demonstrating that this supplement was without detrimental effects on female reproductive healthy mice ( Freitas et al, 2023 ). Supplementation with CLA mixtures in cows increased circulating progesterone, insulin-like growth factor-1, and leptin, improved fertility, and reduced the period from calving to conception ( Csillik et al, 2017 ).…”