Cis Elements: Added Boost to the Directed Evolution of Plant Genes

Abstract

To increase the expression of a native/foreign plant/bacterial gene, the complete network of cis-elements must be excavated to increase its biosynthetic yield, especially under industrial stress conditions. For selecting the best set of cis-elements for a foreign gene and aiding the workflow of researchers, often untrained in bioinformatics methodologies, we developed a modular PERL script for their identification and localization. The script is functional on any operating system. It localizes the cis element network of a gene. It aids an easy customization, as per the required analysis, and provides robust strategy, unlike the usually used databases where several applied calculations often become a tricky task. The script allows an uncomplicated analysis of multiplicity of cis elements along with their relative distances, making it easier for designing the more beneficial network of genes for directed evolution experiments. Through a batched scrutiny of several functionally similar genes, it would aid an easy extraction of their evolutionarily favored network of cis elements. It would be extremely helpful to develop the crop plants that are better adapted to the stressful conditions.

Genome-wide characterization and analysis of Golden 2-Like transcription factors related to leaf chlorophyll synthesis in diploid and triploid Eucalyptus urophylla

“…The cis -acting elements may be activated by trans -acting elements to regulate the activity of the target genes ( Valli et al, 2022 ). Among the cis -acting elements detected in the promoter regions of EgrGLK genes ( Figure 5 ), elements associated with development were only sporadically distributed in the promoter regions, whereas elements involved in light response and hormone response were observed in the promoter regions of almost all EgrGLK genes.…”