Cis-trans-isomerization of violaxanthin in LHC II: violaxanthin isomerization cycle within the violaxanthin cycle

Gruszecki, Wiesław I.; Matuła, Magdalena; Ko-Chi, Naomi; Koyama, Yasushi; Krupa, Zbigniew

doi:10.1016/s0005-2728(96)00167-3

Cited by 27 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite this, the spectral analysis of the dark recovery of the light-driven chlorophyll fluorescence quenching shows that the xanthophyll pigment, which can be tentatively assigned to violaxanthin, undergoes the transformation along with induction of the excitation quenching, which affects its energetic coupling to LHCIIbound chlorophylls. Similar light-dependent effects have also been reported previously (Gruszecki et al, 1994(Gruszecki et al, , 1997Grudzinski et al, 2002). Illumination-induced differences in the chlorophyll a fluorescence excitation spectra in LHCII, demonstrated in Fig.…”

Section: Resultssupporting

confidence: 88%

Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII

Gruszecki

Zubik

Luchowski

et al. 2011

Journal of Plant Physiology

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 88%

Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII

Gruszecki

Zubik

Luchowski

et al. 2011

Journal of Plant Physiology

View full text Add to dashboard Cite

“…The cis-trans isomerization and subsequent detachment of violaxanthin from LHCII to the lipid phase was proposed to be responsible for the light-induced fluorescence quenching (25). This proposal would be consistent with our investigations and especially with the large changes in the entropy of activation for the dark recovery.…”

Section: Possible Origin Of Light-induced Quencherssupporting

confidence: 91%

Kinetic Analysis of the Light-induced Fluorescence Quenching in Light-harvesting Chlorophyll a/b Pigment–Protein Complex of Photosystem II

Barzda¹,

Jennings²,

Zucchelli³

et al. 1999

Photchem. Photbio.

View full text Add to dashboard Cite

We carried out a kinetic analysis of the light-induced fluorescence quenching (AF) of the light-harvesting chlorophyll a/b pigment-protein complex of photosystem 1 1 (LHCII) that was first observed by Jennings et aL (Photosynth. Res. 27, 5764, 1991). We show that during a 2 min light, 2 min dark cycle, both the light and dark phases exhibit blexponential kinetics; this is tentatively explained SAbbreviurions: Chl, chlorophyll; CMC, critical micellar concentration; DM, n-dodecyl p-D-maltoside; AF, light-induced reversible fluorescence quenching; LHCII, light-harvesting chlorophyll a h pigment-protein complex of photosystem 11. 751 752 Virginijus Barzda et a/.

show abstract

“…One possible explanation for the lack of a direct link is that the carotenoids exhibit blue light‐dependent conversion between different isomers. Thus violaxanthin appears to exist in its 15‐ cis form when bound to LHCII (Gruszecki et al . 1997).…”

Section: Discussionmentioning

confidence: 99%

“…In this form its absorption spectrum is almost identical to the action spectra for chloroplast movements in Lemna , including a peak in the UV and multiple peaks between 400 and 500 nm (Molnár & Szabolcs 1993; Zurzycki 1962). Bound violaxanthin undergoes a cis–trans isomerization following a triplet–triplet excitation energy transfer from chlorophyll if there is excess light absorption by the photosynthetic apparatus (Gruszecki et al . 1997), and detaches from LHCII.…”

Section: Discussionmentioning

confidence: 99%

Light perception and the role of the xanthophyll cycle in blue‐light‐dependent chloroplast movements in Lemna trisulca L.

1999

View full text Add to dashboard Cite

Summary In most higher plants, chloroplasts move towards the periclinal cell walls in weak blue light (WBL) to increase light harvesting for photosynthesis, and towards the anticlinal walls as an escape reaction, thus avoiding photo‐damage in strong blue light (SBL). The photo‐ receptor(s) triggering these responses have not yet been identified. In this study, the role of zeaxanthin as a blue‐light photoreceptor in chloroplast movements was investigated. Time‐lapse 3D confocal imaging in Lemna trisulca showed that individual chloroplasts responded to local illumination when one half of the cell was treated with light of different intensity or spectral quality to that received by the other half, or was maintained in darkness. Thus the complete signal perception, transduction and effector system has a high degree of spatial resolution and is consistent with localization of part of the transduction chain in the chloroplasts. Turnover of xanthophylls was determined using HPLC, and a parallel increase was observed between zeaxanthin and chloroplast movements in SBL. Ascorbate stimulated both a transient increase in zeaxanthin levels and chloroplast movement to profile in physiological darkness. Conversely, dithiothreitol blocked zeaxanthin production and responses to SBL and, to a lesser extent, WBL. Norflurazon preferentially inhibited SBL‐dependent chloroplast movements. Increases in zeaxanthin were also observed in strong red light (SRL) when no directional chloroplast movements occurred. Thus it appears that a combination of zeaxanthin and blue light is required to trigger responses. Blue light can cause cis–trans isomerization of xanthophylls, thus photo‐isomerization may be a critical link in the signal transduction pathway.

show abstract

Cis-trans-isomerization of violaxanthin in LHC II: violaxanthin isomerization cycle within the violaxanthin cycle

Cited by 27 publications

References 8 publications

Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII

Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII

Kinetic Analysis of the Light-induced Fluorescence Quenching in Light-harvesting Chlorophyll a/b Pigment–Protein Complex of Photosystem II

Light perception and the role of the xanthophyll cycle in blue‐light‐dependent chloroplast movements in Lemna trisulca L.

Contact Info

Product

Resources

About