Cisd2 Plays an Essential Role in Corneal Epithelial Regeneration

Sun, Chi; Lee, Shao-Yun; Kao, Cheng‐Heng; Chen, Li‐Hsien; Shen, Zhao-Qing; Lai, Chia-Yun; Tzeng, Tsai-Yu; Pang, Jong‐Hwei S.; Chiu, Wen‐Tai; Tsai, Ting‐Fen

doi:10.2139/ssrn.3895624

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“…The expression of K12 was significantly decreased in the corneal epithelium of Aqp5 −/− mice, whereas the expression of K1, K10, and K14 was increased. K12 is a marker of corneal epithelium, can be detected in the entire corneal epithelium and on the upper margin of the basal epithelium 36 but not in the adjacent conjunctiva or the limbus basal epithelium. K14 is a marker of the epithelial cell differentiation, which is only expressed in limbus basal cells and the basal layer of the corneal epithelium 37 .…”

Section: Introductionmentioning

confidence: 99%

Loss of aquaporin 5 contributes to the corneal epithelial pathogenesis via Wnt/β‐catenin pathway

Wang

Zhang

et al. 2023

The FASEB Journal

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AQP5 plays a crucial role in maintaining corneal transparency and the barrier function of the cornea. Here, we found that in the corneas of Aqp5−/− mice at older than 6 months, loss of AQP5 significantly increased corneal neovascularization, inflammatory cell infiltration, and corneal haze. The results of immunofluorescence staining showed that upregulation of K1, K10, and K14, and downregulation of K12 and Pax6 were detected in Aqp5−/− cornea and primary corneal epithelial cells. Loss of AQP5 aggravated wound‐induced corneal neovascularization, inflammation, and haze. mRNA sequencing, western blotting, and qRT‐PCR showed that Wnt2 and Wnt6 were significantly decreased in Aqp5−/− corneas and primary corneal epithelial cells, accompanied by decreased aggregation in the cytoplasm and nucleus of β‐catenin. IIIC3 significantly suppressed corneal neovascularization, inflammation, haze, and maintained corneal transparent epithelial in Aqp5−/− corneas. We also found that pre‐stimulated Aqp5−/− primary corneal epithelial cells with IIIC3 caused the decreased expression of K1, K10, and K14, the increased expression of K12, Pax6, and increased aggregation in the cytoplasm and nucleus of β‐catenin. These findings revealed that AQP5 may regulate corneal epithelial homeostasis and function through the Wnt/β‐catenin signaling pathway. Together, we uncovered a possible role of AQP5 in determining corneal epithelial cell fate and providing a potential therapeutic target for corneal epithelial dysfunction.

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Section: Introductionmentioning

confidence: 99%