Cisplatin resistance and malignant behaviors of lung cancer cells are promoted by circ_0002360 via targeting miR‐6751‐3p to regulate the expression of ZNF300

Abstract

Background: Circular RNAs (circRNAs) are key regulators in oncogenesis and chemoresistance of human cancers. Herein, we focused on the roles of circ_0002360 in regulating lung cancer progression and cisplatin (DDP) resistance. Methods: The detection of circ_0002360, microRNA-6751-3p (miR-6751-3p) and zinc finger protein 300 (ZNF300) was conducted via reverse transcription-quantitative polymerase chain reaction assay. Cell sensitivity was determined using cell counting kit-8 assay. Proliferation detection was performed by colony formation assay and EdU assay. The migrated cells were examined via transwell assay. Cell apoptosis analysis was carried out through flow cytometry and caspase 3 activity assay. Western blot was used to examine the protein levels. Target interaction was confirmed through dualluciferase reporter assay and RNA immunoprecipitation assay. Circ_0002360 function in vivo was performed via xenograft tumor assay. Results: Circ_0002360 was overexpressed in DDP-resistant lung cancer tissues and cells. Silencing circ_0002360 inhibited DDP resistance, proliferation and migration but enhanced apoptosis of DDP-resistant cells. Circ_0002360 could elevate ZNF300 expression. DDP resistance and lung cancer progression were also impeded by ZNF300 downregulation. ZNF300 overexpression reversed the function of circ_ 0002360 knockdown in DDP-resistant lung cancer cells. The regulation of circ_0002360 for ZNF300 was achieved by sponging miR-6751-3p. Circ_0002360 promoted DDP resistance in xenograft mice through mediating the miR-6751-3p/ZNF300 axis. Conclusion: Circ_0002360 targeted miR-6751-3p to regulate ZNF300 level, thus elevating DDP resistance and promoting the malignant progression of lung cancer cells.

Circular RNA circ_0002360 regulates the Taxol resistance and malignant behaviors of Taxol-resistant non-small cell lung cancer cells by microRNA-585-3p-dependent modulation of G protein regulated inducer of neurite outgrowth 1

“…Circ_0002360 can function as a potent oncogenic promoter in NSCLC by elevating the expression of matrix metalloproteinase 16 (MMP16) by sponging multiple miRNAs [ 14 ]. Moreover, circ_0002360 accelerates the cisplatin resistance and malignant phenotypes of lung cancer cells through the miR-6751-3p/ZNF300 axis [ 15 ]. In this work, our data found that circ_0002360 was evidently overexpressed in Taxol-resistant NSCLC.…”

“…Human circ_0002360, generated by the back-spliced exons of RUNX family transcription factor 1 (RUNX1), is highly expressed in NSCLC and operates as a promoter in NSCLC [ 13 , 14 ]. Moreover, circ_0002360 can contribute to the cisplatin resistance and malignant phenotypes of lung cancer cells by upregulating zinc finger protein 300 (ZNF300) through miR-6751-3p competition [ 15 ]. However, no studies proved the function of circ_0002360 in the Taxol resistance of NSCLC.…”

Identifying diagnostic markers and constructing a prognostic model for small-cell lung cancer based on blood exosome-related genes and machine-learning methods

“…The high abundance, stability, and conservation of circRNAs make them advantageous as diagnostic markers ( 37 ). It has been reported that hsa_circ_0002360 is highly expressed in NSCLC tissues and contributes to the malignant behavior of NSCLC ( 38 – 40 ). Circulating hsa_circ_0001953 can be used as a marker for proliferative diabetic retinopathy and active tuberculosis, which have not been investigated in cancer ( 41 , 42 ).…”