Cisplatin resistance‑associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

Zhou, Shuping; Wei, Juan; Wang, Yeliu; Liu, Xinkuang

doi:10.3892/etm.2020.8526

Cited by 26 publications

(21 citation statements)

References 30 publications

(26 reference statements)

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“…As reported by a lot of studies, there is a correlation between abnormally expressed circRNAs and clinical features, with the former possibly becoming a biomarker for NSCLC [23][24][25] . circRNA_101237, a novel identified circRNA, whose encoding gene is located at chromosome (chr) 13:26974589-26975761 and which is produced by backsplicing of exons 10, 11 and 12 of cyclin-dependent kinase (CDK) 8, has been reported to be implicated in Cisplatin resistance-associated of HCC 8 . Nevertheless, we still do not well understand how circRNA_101237 affects NSCLC as well as the potential mechanisms it holds.…”

Section: Discussionmentioning

confidence: 99%

CircRNA_101237 promotes NSCLC progression via the miRNA-490-3p/MAPK1 axis

Zhang

Gao

et al. 2020

Sci Rep

114

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Non-small cell lung cancer (NSCLC) is a common type of lung cancer, characterized by a poor prognosis. In the last several years, more and more studies have demonstrated the significant roles played by circular RNAs (circRNAs) in different human tumors progression including NSCLC. The present study was to explore the mechanism of hsa_circ_101237 in regulating non-small cell lung cancer (NSCLC). Totally 303 NSCLC cases were enrolled. A549 and H1299 cells were transfected. Cells viability, migration and invasion were determined by CCK-8 assay and transwell experiment, respectively. Luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were performed. hsa_circ_101237, miR-490-3p and MAPK1 expression in tissues/cells were detected by qRT-PCR. The study found an elevation in the expression of Hsa_circRNA_101237 in both NSCLC tissues and cell line. High Hsa_circRNA_101237 expression predicted poor survival in NSCLC. Meanwhile, we found that hsa_ circRNA_101237 expression sponged miR-490-3p to enhance MAPK1 expression, thus significantly promoting NSCLC cell lines proliferation, migration, and invasion. MAPK1 restoration prevented NSCLC cells proliferation, migration, and invasion to be repressed due to hsa_circRNA_101237 knockdown. To sum up, as revealed by the study, hsa_circRNA_101237 promoted the expression of MAPK1 via miRNA-490-3p sponge, thus affecting the NSCLC as an important onco-circRNA. Lung cancer, characterized by a poor prognosis but a high morbidity, is a common malignant tumor, non-small cell lung cancer (NSCLC) are identified as the most common type of lung cancer (occupying about 85%) 1. As is well known, smoking is the main cause of NSCLC 2. As NSCLC does not show obvious clinical symptoms and the screening programs are not effective, most patients with NSCLC, once they are diagnosed, are at advanced stages with a poor prognosis 3. More and more clinical studies found that metastasis greatly hinders the treatment of NSCLC cancer; therefore, in-depth understanding the metastasis mechanisms is beneficial for effectively treating NSCLC. Circular RNAs (circRNAs) are a kind of non-coding RNAs with endogenous and conserve, forming a covalently closed continuous loop via back-splice without 3ʹ-end or 5ʹ-end 4. Due to the feature, circRNAs exhibit a lot of properties, and many properties were found not long ago. circRNAs have a special closed loop structure, making them able to resist the degradation medicated by exonuclease. On that account, they are more stable compared with most other linear RNAs, thus they can be used as a biomarker to effectively diagnose and treat cancers 5. circRNAs can interact with RNA-binding proteins, so as to regulate target gene expression 6. Besides, it has been indicated that circRNAs can be sinks for miRNAs, to control the function processed by miRNAs 7. Many human cancers see altered circRNA expression, and many researches have revealed the key role played by circR-NAs in the tumorigenesis. circRNA_101237, a new circRNA identified recently, whose encoding ge...

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Section: Discussionmentioning

confidence: 99%

CircRNA_101237 promotes NSCLC progression via the miRNA-490-3p/MAPK1 axis

Zhang

Gao

et al. 2020

Sci Rep

114

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“…For example, circRNA_101237 is a prognostic biomarker associated with resistance to cisplatin in hepatocellular carcinoma. 21 In addition, circFBXO11 regulates the progression and oxaliplatin resistance of hepatocellular carcinoma by modulating the miR‐605/FOXO3/ABCB1 axis, 22 whereas hsa_circ_0079662 induces human colon cancer cell resistance to oxaliplatin via affecting the TNF‐α pathway. 23 Sequencing studies have also found that mRNA, circRNA, and lncRNA expression profiles in extracellular vesicles derived from human serum samples offer promise as diagnostic cancer biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Clinical Significance of has_circ_0055625 in Colon Cancer Patients Undergoing FOLFOX Chemotherapy Treatment

Gao

Shi

et al. 2021

IJGM

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Objective: Colon cancer is a leading global cancer-related cause of morbidity and mortality. The oxaliplatin, 5-fluorouracil (5-FU), and leucovorin (FOLFOX) regimen is a standard chemotherapeutic approach used to treat colon cancer. However, chemoresistant tumor cells typically lead to the emergence of recurrent FOLFOX-resistant tumors after initial treatment. As such, it is vital that novel approaches to identifying and eliminating such chemoresistant tumors be developed in an effort to improve patient chemotherapy outcomes. Materials and Methods: In total, 100 samples of serum were obtained between April 2014 and April 2019 from patients who had been pathologically diagnosed with colon cancer from the Xiamen Haicang Hospital, and after these patients received FOLFOX chemotherapy treatment, serum samples were collected again. The expression of has_circ_0055625 in these serum samples was assessed via qPCR. Additionally, 5-FU IC50 values were detected via CCK-8 assay. Results: We found has_circ_0055625 to be significantly upregulated in colon cancer patient serum. After FOLFOX treatment, chemotherapy-resistance was associated with the upregulation of has_circ_0055625. Conclusion:In summary, these data may provide a foundation for future studies of chemotherapeutic resistance in patients undergoing FOLFOX treatment, potentially guiding treatment adjustment strategies. However, further work will be necessary to expand upon these findings.

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“…Of interest was the observation that cisplatin induced expression of the circRNA. 127 hsa_circ_0027089 Microarray analysis was carried out on plasma samples from 10 patients with HBV-related HCC and 5 patients with HBV-related cirrhosis. Of the panel of up- and down-regulated circRNAs, hsa_circ_0027089 was identified as a potentially useful diagnostic marker that distinguishes HCC from cirrhosis in patients with chronic HBV infection.…”

Section: Circrnas Implicated In the Etiology Of Hepatocellular Carcinmentioning

confidence: 99%

“… 129–132 Upregulated and downregulated circRNAs were identified, then measured in patients’ samples to correlate with features of tumor cell proliferation, invasion and spread. More recently screening of RNA extracted from tumor and normal tissue using microarray analysis 119 , 124 , 125 , 127 , 128 has also been performed. These analyses were followed by verification using RT-qPCR.…”

Section: Circrnas Implicated In the Etiology Of Hepatocellular Carcinmentioning

confidence: 99%

“…Obtaining liver tissue is an invasive procedure that has complications, which makes less invasive procedures preferable. To address this, investigators have measured circulating RNAs, 122–125 , 127 , 128 , 133 which may be located in exosomes derived from malignant liver cells. Correlation between profiles observed in blood samples and malignant liver cells provided validation of circRNAs’ utility as biomarkers.…”

Section: Circrnas Implicated In the Etiology Of Hepatocellular Carcinmentioning

confidence: 99%

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Recent Update on the Role of Circular RNAs in Hepatocellular Carcinoma

Ely¹,

Bloom²,

Maepa³

et al. 2021

JHC

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After being overlooked for decades, circular RNAs (circRNAs) have recently generated considerable interest. circRNAs play a role in a variety of normal and pathological biological processes, including hepatocarcinogenesis. Many circRNAs contribute to hepatocarcinogenesis through sponging of microRNAs (miRs) and disruption of cellular signaling pathways that play a part in control of cell proliferation, metastasis and apoptosis. In most cases, overexpressed circRNAs sequester miRs to cause de-repressed translation of mRNAs that encode oncogenic proteins. Conversely, low expression of circRNAs has also been described in hepatocellular carcinoma (HCC) and is associated with inhibited production of tumor suppressor proteins. Other functions of circRNAs that contribute to hepatocarcinogenesis include translation of truncated proteins and acting as adapters to regulate influence of transcription factors on target gene expression. circRNAs also affect hepatocyte transformation indirectly. For example, the molecules regulate immune surveillance of cancerous cells and influence the liver fibrosis that commonly precedes HCC. Marked over- or under-expression of circRNA expression in HCC, with correlating plasma concentrations, has diagnostic utility and assays of these RNAs are being developed as biomarkers of HCC. Although knowledge in the field has recently surged, the myriad of described effects suggests that not all may be vital to hepatocarcinogenesis. Nevertheless, investigation of the role of circRNAs is providing valuable insights that are likely to contribute to improved management of a serious and highly aggressive cancer.

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Cisplatin resistance‑associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

Cited by 26 publications

References 30 publications

CircRNA_101237 promotes NSCLC progression via the miRNA-490-3p/MAPK1 axis

CircRNA_101237 promotes NSCLC progression via the miRNA-490-3p/MAPK1 axis

Clinical Significance of has_circ_0055625 in Colon Cancer Patients Undergoing FOLFOX Chemotherapy Treatment

Recent Update on the Role of Circular RNAs in Hepatocellular Carcinoma

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