Cisplatin suppresses proliferation, migration and invasion of nasopharyngeal carcinoma cells in vitro by repressing the Wnt/β-catenin/Endothelin-1 axis via activating B cell translocation gene 1

Yin, Peng; Song, Guizhen; Jiang, Zhenhua

doi:10.1007/s00280-018-3536-5

Cited by 13 publications

(13 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well documented that chemotherapy drugs contain anti-tumor activities involved in the inhibition of the proliferation, induced cell apoptosis, or suppressed cell metastasis of tumor cells [ 30 , 31 , 32 , 33 ]. Numerous pieces of evidence have shown that tumor metastasis may lead to multiple organ failures and dyscrasia, which are the leading causes of death in patients with malignant tumors.…”

Section: Discussionmentioning

confidence: 99%

Berberine Inhibits Human Melanoma A375.S2 Cell Migration and Invasion via Affecting the FAK, uPA, and NF-κB Signaling Pathways and Inhibits PLX4032 Resistant A375.S2 Cell Migration In Vitro

et al. 2018

View full text Add to dashboard Cite

Many studies have demonstrated that berberine inhibited the cell migration and invasion in human cancer cell lines. However, the exact molecular mechanism of berberine inhibiting the cell migration and invasion of human melanoma A375.S2 and A375.S2/PLX (PLX4032 induced resistant A375.S2) skin cancer cells remains unknown. In this study, we investigated the anti-metastasis mechanisms of berberine in human melanoma cancer A375.S2 cells and A375.S2/PLX resistant cells in vitro. Berberine at low concentrations (0, 1, 1.5 and 2 μM) induced cell morphological changes and reduced the viable cell number and inhibited the mobility, migration, and invasion of A375.S2 cells that were assayed by wound healing and transwell filter. The gelatin zymography assay showed that berberine slightly inhibited MMP-9 activity in A375.S2 cells. Results from western blotting indicated that berberine inhibited the expression of MMP-1, MMP-13, E-cadherin, N-cadherin, RhoA, ROCK1, SOS-1, GRB2, Ras, p-ERK1/2, p-c-Jun, p-FAK, p-AKT, NF-κB, and uPA after 24 h of treatment, but increased the PKC and PI3K in A375.S2 cells. PLX4032 is an inhibitor of the BRAFV600E mutation and used for the treatment of cancer cells harboring activated BRAF mutations. Berberine decrease cell number and inhibited the cell mobility in the resistant A375.S2 (A375.S2/PLX, PLX4032 generated resistant A375.S2 cells). Based on these observations, we suggest that the potential of berberine as an anti-metastatic agent in melanoma that deserves to be investigated in more detail, including in vivo studies in future.

show abstract

Section: Discussionmentioning

confidence: 99%

Berberine Inhibits Human Melanoma A375.S2 Cell Migration and Invasion via Affecting the FAK, uPA, and NF-κB Signaling Pathways and Inhibits PLX4032 Resistant A375.S2 Cell Migration In Vitro

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The mechanism of action of cDDP has been linked to its ability to induce DNA damage via the formation of crosslinks with the purine bases on DNA, interfering with DNA repair mechanisms, and subsequently inducing the apoptosis of cancer cells. Moreover, multiple signal transduction pathways are activated by cDDP-induced genotoxic stress, through the activation of MAPK pathways, particularly JNK and ERK (13,52). These mechanisms may contribute to either apoptosis or chemoresistance.…”

Section: Discussionmentioning

confidence: 99%

“…These mechanisms may contribute to either apoptosis or chemoresistance. Furthermore, it was recently demonstrated that cDDP has the ability to interfere with the proliferation, migration and invasion of nasopharyngeal carcinoma cells in vitro by suppressing the Wnt/β-catenin/Endothelin-1 axis via the activation of the B cell translocation gene 1 (52).…”

Section: Discussionmentioning

confidence: 99%

Effects of S‑adenosyl‑L‑methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms

et al. 2020

View full text Add to dashboard Cite

S-Adenosyl-L-methionine (AdoMet) is the principal methyl donor in transmethylation reactions fundamental to sustaining epigenetic modifications. Over the past decade, AdoMet has been extensively investigated for its antiproliferative, pro-apoptotic and anti-metastatic roles in several types of human cancer. Head and neck squamous cell carcinoma (HNSCC) is the sixth most common type of cancer worldwide, and is an aggressive type of cancer that is associated with a high recurrence rate, metastasis and poor treatment outcomes. The present study demonstrates, for the first time, to the best of our knowledge, that AdoMet induces cell cycle arrest and inhibits the migratory and invasive ability of two different HNSCC cell lines, oral Cal-33 and laryngeal JHU-SCC-011 cells. In both cell lines, AdoMet attenuated cell cycle progression, decreased the protein level of several cyclins and downregulated the expression of p21 cell cycle inhibitor. Moreover, AdoMet was able to inhibit Cal-33 and JHU-SCC-011 cell migration in a dose-dependent manner after 24 and 48 h, respectively, and also induced a significant reduction in the cell invasive ability, as demonstrated by Matrigel invasion assay monitored by the xCELLigence RTCA system. Western blot analysis of several migration and invasion markers confirmed the inhibitory effects exerted by AdoMet on these processes and highlighted AKT, β-catenin and small mothers against decapentaplegic (SMAD) as the main signaling pathways modulated by AdoMet. The present study also demonstrated that the combination of AdoMet and cisplatin synergistically inhibited HNSCC cell migration. Taken together, these findings demonstrate that the physiological compound, AdoMet, affects the motility and extracellular matrix invasive capability in HNSCC. Thus, AdoMet may prove to be a good candidate for future drug development against metastatic cancer.

show abstract

“…The reason is due to metastatic lesions in the lungs, multi-drug resistance and the lack of better molecular biomarkers to detect OS at an early stage (34). Some of the chemotherapy drugs were developed to inhibit tumor cell proliferation, apoptosis or metastasis (35)(36)(37). Thus, blockage of the signaling pathways leading to cancer cell metastasis is one of best strategies against cancer cells (37,38).…”

Section: Discussionmentioning

confidence: 99%

“…Some of the chemotherapy drugs were developed to inhibit tumor cell proliferation, apoptosis or metastasis (35)(36)(37). Thus, blockage of the signaling pathways leading to cancer cell metastasis is one of best strategies against cancer cells (37,38). To this end, natural products are being tested for the treatment of OS.…”

Section: Discussionmentioning

confidence: 99%

Fisetin Suppresses Human Osteosarcoma U-2 OS Cell Migration and InvasionviaAffecting FAK, uPA and NF-ĸB Signaling PathwayIn Vitro

Chen

Peng

Lai

et al. 2019

In Vivo

View full text Add to dashboard Cite

Background/Aim: Evidence has indicated that fisetin induces cytotoxic effects in human cancer cell lines, including the inhibition of cell migration and invasion, however, the exact molecular mechanism of action of fisetin in human osteosarcoma cells remains unclear. Materials and Methods: The anti-metastatic mechanisms of fisetin in human osteosarcoma U-2 OS cells were investigated in vitro. Results: Fisetin reduced the viability of cells at different concentrations (2.5, 5 and 10 μM) as measured by flow cytometric assay. Fisetin suppressed cell mobility, migration and invasion of U-2 OS cells, as shown by wound healing assay and transwell filter chambers, respectively. The gelatin zymography assay showed that fisetin inhibited MMP-2 activity in U-2 OS cells. Results from western blotting indicated that fisetin reduced the levels

show abstract

Cisplatin suppresses proliferation, migration and invasion of nasopharyngeal carcinoma cells in vitro by repressing the Wnt/β-catenin/Endothelin-1 axis via activating B cell translocation gene 1

Cited by 13 publications

References 47 publications

Berberine Inhibits Human Melanoma A375.S2 Cell Migration and Invasion via Affecting the FAK, uPA, and NF-κB Signaling Pathways and Inhibits PLX4032 Resistant A375.S2 Cell Migration In Vitro

Berberine Inhibits Human Melanoma A375.S2 Cell Migration and Invasion via Affecting the FAK, uPA, and NF-κB Signaling Pathways and Inhibits PLX4032 Resistant A375.S2 Cell Migration In Vitro

Effects of S‑adenosyl‑L‑methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms

Fisetin Suppresses Human Osteosarcoma U-2 OS Cell Migration and InvasionviaAffecting FAK, uPA and NF-ĸB Signaling PathwayIn Vitro

Contact Info

Product

Resources

About