Cistus incanus Root Organ Cultures: a Valuable Tool for Studying Mycorrhizal Associations

Coughlan, Andrew P.; Piché, Yves

doi:10.1007/3-540-27331-x_13

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…Fortas and Chevalier (1992) reported that phosphorus concentration was important in determining the type of mycorrhiza formed between three truffle species and H. guttatum under in-vitro culture conditions; whether this might be important in natural systems is not known. Although a few studies have explored plant-fungus signaling in the Cistaceae, these have only involved Cistus species and mechanisms underlying these interactions are still being investigated (see Coughlan and Piché 2005).…”

Section: Discussionmentioning

confidence: 99%

Hudsonia ericoides and Hudsonia tomentosa: Anatomy of mycorrhizas of two members in the Cistaceae from Eastern Canada

Massicotte

Peterson

Melville

et al. 2010

Botany

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Most species in the family Cistaceae are found in the Mediterranean basin. Several hosts are of special interest, owing to their associations with truffle species, while many are important as pioneer plants in disturbed areas and in soil stabilization. For these reasons, understanding their root systems and their associated fungal symbionts is important. Most studies of the structure of mycorrhizas in this family involve two genera, Cistus and Helianthemum. The present study examines structural features of mycorrhizas in two North American species, Hudsonia ericoides L. and Hudsonia tomentosa Nutt. Root systems of both species are highly branched with most fine roots colonized by mycorrhizal fungi. Based on morphological features, several mycorrhizal fungi were identified; structural details also provided evidence of more than one fungal symbiont for each host species. All mycorrhizas had a multi-layered fungal mantle and Hartig net hyphae confined to radially elongated epidermal cells; no intracellular hyphae were observed. Although the Hartig net was confined to the epidermis, the outer row of cortical cell walls lacked suberin, a known barrier to fungal penetration. Mycorrhizas in H. ericoides and H. tomentosa differed from those of Cistus and Helianthemum species that have a Hartig net that extends into the root cortex, as well as frequently present intracellular hyphae.Résumé : La plupart des espèces de la famille des Cistaceae se retrouvent dans le bassin de la Méditerranée. Plusieurs hô-tes présentent un intérêt particulier, compte tenu de leurs associations avec des espèces de truffes, alors que d'autres constituent des plantes pionnières importantes dans les endroits perturbés et pour la stabilisation des sols. Pour ces raisons, il importe de comprendre la biologie de leurs systèmes racinaires et de leurs champignons symbiotiques associés. La plupart des études publiées sur la structure des mycorhizes de cette famille portent sur les deux genres Cistus et Helianthemum. On examine ici les caractéristiques structurales des mycorhizes de deux espèces Nord-américaines, l'Hudsonia ericoides L. et l'Hudsonia tomentosa Nutt. Chez les deux espèces, l'on observe des systèmes racinaires fortement ramifiés, la plupart des racines fines étant colonisées par des champignons mycorhiziens. Sur la base des caractéristiques morphologiques, les auteurs ont pu identifier plusieurs espèces de champignons mycorhiziens; les détails des structures fournissent également des preuves de l'existence de plus d'un symbiote fongique chez chaque espèce hôte. Toutes les mycorhizes montrent un manteau fongique à plusieurs couches et des hyphes du réseau de Hartig confinées aux cellules épidermiques radialement allongées; on observe aucune pénétration intracellulaire. Bien que le réseau de Hartig soit confiné à l'épi-derme, les parois cellulaires de la couche externe de cellules corticales ne possèdent pas de subérine, une barrière reconnue pour la pénétration fongique. Les mycorhizes chez les H. ericoides et H. tomentosa diffèrent de ce...

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Section: Discussionmentioning

confidence: 99%

Hudsonia ericoides and Hudsonia tomentosa: Anatomy of mycorrhizas of two members in the Cistaceae from Eastern Canada

Massicotte

Peterson

Melville

et al. 2010

Botany

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“…Repeated in vitro subculturing on synthetic media is time-consuming, expensive, and prone to contamination, and it does not prevent some biological changes, such as the loss of host infectivity or fruiting ability. 15,16,24 Studies evaluating the efficiency of storage methods in maintaining the biological features of fungal strains are unfortunately relatively rare. 11 Storage in liquid nitrogen and ULF are generally considered the most reliable strategies for long-term preservation of filamentous mushrooms.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, storage in Petri dishes of repeated subculture of pathogenic and mycorrhizal fungi leads to decreased mycelial infectivity. 15,16 Preservation at −80°C was successfully tested on mycelia of the medicinal royal sun mushroom, Agaricus blazei Murrill ss. Heinem.…”

Section: A U T H O R P R O O Fmentioning

confidence: 99%

Ultra-Low Freezing to Preserve the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes)

Leonardi

Puliga

Iotti

et al. 2018

Int J Med Mushrooms

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Ganoderma lucidum (Curtis) P. Karst., commonly used in traditional Chinese medicine, is characterized by strong genetic and phenotypic variability that reflects its active components. To preserve such a source of pharmacologically active metabolites, specimens must be collected from different geographic regions and their genetic integrity ensured during storage. To this aim, we tested the effect of ultra-low freezing (ULF) at -120°C on the vitality, mycelial growth rate, and fruiting ability of 3 Italian strains of G. lucidum. Results showed that all strains reacted positively to ULF, demonstrating an ability to recover after 3 months of storage without morphological or physiological changes occurring, regardless of treatment. The successful storage of G. lucidum at -120°C opens up the possibility to create a germplasm bank to collect strains of this medicinal fungus from throughout Europe, thereby contributing to the maintenance of its diversity.

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The Use of Mycorrhizal Biotechnology in Restoration of Disturbed Ecosystem

Quoreshi

2008

Mycorrhizae: Sustainable Agriculture and Forestry

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Cistus incanus Root Organ Cultures: a Valuable Tool for Studying Mycorrhizal Associations

Cited by 5 publications

References 42 publications

Hudsonia ericoides and Hudsonia tomentosa: Anatomy of mycorrhizas of two members in the Cistaceae from Eastern Canada

Hudsonia ericoides and Hudsonia tomentosa: Anatomy of mycorrhizas of two members in the Cistaceae from Eastern Canada

Ultra-Low Freezing to Preserve the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes)

The Use of Mycorrhizal Biotechnology in Restoration of Disturbed Ecosystem

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