Citric acid, but not tetracycline, improves the microscopic pattern of healing of particulate autogenous bone grafts in critical‐size defects

Abstract

Background: Bone demineralization has shown to be advantageous in autogenous onlay bone grafts and in pre-osteoblasts cultures, but such procedure has never been evaluated in particulate bone grafts. This study aimed to investigate the role of two demineralizing agents in the repair of the 8-mm critical-size defects in rats' calvaria. Methods: Eighty adult male Wistar rats were randomly assigned to one of eight groups as follows: particulate autogenous bone demineralized with citric acid for 15 seconds (CA15), 30 seconds (CA30), or 60 seconds (CA60); particulate autogenous bone demineralized with tetracycline hydrochloride for 15 seconds (TCN15), 30 seconds (TCN30), or 60 seconds (TCN60); blood clot (NC), and nondemineralized autogenous bone (PC). The calvariae were harvested at 30 and 60 postoperative days (n = 5) for blinded histological and histometric analysis of the percentage area of newly formed bone within the defects. Results:In the NC and TCN groups, bone formation was limited to the margins of the defects at 30 postoperative days, whereas complete closure was present in all the specimens from CA15 group. Both at 30 and 60 postoperative days, histomorphometry showed significant higher area of newly formed bone in specimens demineralized with CA than in those demineralized with TCN or non-demineralized (P < 0.05). TCN appeared to impair bone neoformation, as its use produced similar or inferior results compared to blood clot.Conclusions: Demineralization of particulate bone grafts with CA during 15s enhanced the regeneration of critical-size defects and may be a promising adjuvant in regenerative procedures. TCN seems to be improper for this purpose.

Enhancing osteogenic differentiation of MC3T3-E1 cells during inflammation using UPPE/β-TCP/TTC composites via the Wnt/β-catenin pathway

“… 39 In recent years, researchers have conducted several studies on the application of TTC in the repair of periodontitis-related bone defects, yielding significant advances. 40 For instance, studies have demonstrated that TTC can alleviate the symptoms of periodontitis by exerting anti-inflammatory effects and facilitating localized tissue repair. 41 TTC can inhibit inflammatory responses and cartilage resorption, thereby mitigating the infiltration and destruction of inflammatory cells and consequently mitigating bone destruction and bone defects.…”

Scanning electron microscopic analysis of 5% glycolic acid as a novel alternative to 17% ethylenediaminetetraacetic acid in root biomodification: An in vitro study

“…RB provides a stable substrate that is devoid of contaminants, acts as a chemotactic attractant for fibroblasts, eliminating the toxic substances that inhibit the proliferation and attachment of gingival fibroblasts to the root surface, enabling new connective tissue attachment formation or regeneration. [ 4 5 ] Although various RB agents, including citric acid, ethylenediaminetetraacetic acid (EDTA), fibronectin, and antibiotics such as tetracyclines and LASERs, have been widely tested, citric acid and EDTA have been most widely accepted in clinical use. [ 6 7 8 9 10 11 ] Citric acid is effective against smear layer removal and is found to have strong antimicrobial properties; however, it lacks chemical stability necessary for regenerative potential.…”

Efeito da desmineralização por ácido cítrico do enxerto ósseo autógeno particulado no reparo de defeitos infra-ósseos em seres humanos: estudo clínico

“…O atual projeto, portanto, faz parte dessa linha de pesquisa inovadora que vem sendo ampliada gradativamente estimulada pelos resultados positivos alcançados previamente (RODRIGUES et al, 2012;DOMINGUES, 2013;REZENDE et al, 2014;REZENDE et al, 2015;SALMERON, 2015;ROJAS-PAULÚS, 2016;MANFREDI, 2016;CARVALHO, 2017;CAETANO, 2019;MANFREDI et al, 2019;CARVALHO et al, 2020;ROJAS-PAULÚS et al, 2021). A proposta deste estudo é testar a hipótese de que a desmineralização intencional, com ácido cítrico, do enxerto ósseo autógeno particulado traz resultados tão favoráveis quanto o processo fisiológico de reparo ósseo, ou ainda melhores, no que se refere ao tempo de neoformação óssea e qualidade do osso neoformado.…”

“…Estudos prévios que demonstraram resultados favoráveis para o grupo teste onde foi realizada a desmineralização dos enxertos (REZENDE, 2008;COESTA, 2011;RODRIGUES et al, 2012;DOMINGUES, 2013;REZENDE et al, 2014;REZENDE et al, 2015;SALMERON, 2015;MANFREDI, 2016;ROJAS-PAULÚS, 2016;CARVALHO, 2017;CAETANO, 2019;MANFREDI et al, 2019;CARVALHO et al, 2020;ROJAS-PAULÚS et al, 2021) Além disso, os estudos prévios mencionados realizaram diferentes períodos de avaliação e foram capazes de detectar mudanças no tecido ósseo mais precocemente com 24, 48 e 72h (COESTA, 2011;SALMERON, 2015;MANFREDI, 2016;MANFREDI et al, 2019), aos 7, 30 e 60 dias (REZENDE, 2008;REZENDE et al, 2014;de REZENDE et al, 2015;ROJAS-PAULÚS, 2016;CARVALHO, 2017;CARVALHO et al, 2020;ROJAS-PAULÚS et al, 2021) e aos 30 e 45 dias (DOMINGUES, 2013).…”