Citric acid-derived in situ crosslinkable biodegradable polymers for cell delivery

Gyawali, Dipendra; Nair, Parvathi; Zhang, Yi; Tran, Richard T.; Zhang, Chi; Samchukov, Mikhail; Makarov, Marina R.; Kim, Harry K.W.; Yang, Jian

doi:10.1016/j.biomaterials.2010.08.022

Cited by 135 publications

(120 citation statements)

References 45 publications

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“…Moreover, poly citric acid is a highly biocompatible polymer. 32,33 Results of infrared, 1 H NMR, and 13 C NMR confirmed conjugation of paclitaxel to the carboxyl functional groups of the poly citric acid branches on the surface of MWNTs via a cleavable ester bond.…”

Section: Discussionmentioning

confidence: 99%

Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Sobhani¹,

Dinarvand²,

Atyabi³

et al. 2011

IJN

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Potential applications of carbon nanotubes have attracted many researchers in the field of drug delivery systems. In this study, multiwalled carbon nanotubes (MWNTs) were first functionalized using hyperbranched poly citric acid (PCA) to improve their hydrophilicity and functionality. Then, paclitaxel (PTX), a potent anticancer agent, was conjugated to the carboxyl functional groups of poly citric acid via a cleavable ester bond to obtain a MWNT- g -PCA-PTX conjugate. Drug content of the conjugate was about 38% (w/w). The particle size of MWNT- g -PCA and MWNT- g -PCA-PTX was approximately 125 and 200 nm, respectively. Atomic force microscopy and transmission electron microscopy images showed a curved shape for MWNT- g -PCA and MWNT- g -PCA-PTX, which was in contrast with the straight or linear conformation expected from carbon nanotubes. It seems that the high hydrophilicity of poly citric acid and high hydrophobicity of MWNTs led to conformational changes from a linear state to a curved state. Paclitaxel can be released from the MWNT- g -PCA-PTX conjugates faster at pH 6.8 and 5.0 than at pH 7.4, which was suitable for the release of the drug in tumor tissues and tumor cells. In vitro cytotoxicity studies were evaluated in the A549 and SKOV3 cell lines. MWNT- g -PCA had an insignificant cytotoxic effect on both cell lines. MWNT- g -PCA-PTX had more of a cytotoxic effect than the free drug over a shorter incubation time (eg, 24 hours versus 48 hours), which suggests improved cell penetration of MWNT- g -PCA-PTX. Therefore, paclitaxel conjugated to MWNT- g -PCA is promising for cancer therapeutics.

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Section: Discussionmentioning

confidence: 99%

Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Sobhani¹,

Dinarvand²,

Atyabi³

et al. 2011

IJN

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“…This behavior is very important in the delivery and release of cells and biomolecules for therapeutic approaches (8)(9)(10) and medical diagnostic tests because cell-controlled degradability and biomolecule release depend on swelling. For example, polymer microspheres with immobilized proteins have been used in optical biosensors to detect antibodies (11) and a pointof-care diagnostic device for the prevention of diseases has been successfully developed using microdropletbased printing technique (12).…”

Section: Introductionmentioning

confidence: 99%

Swelling characterization of photo-cross-linked gelatin methacrylate spherical microgels for bioencapsulation

Jung

2014

E-Polymers

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The swelling behavior of biocompatible and biodegradable polymers is important for the delivery and release of cells and drugs in biomedical applications. This study reported the swelling characteristics of photo-cross-linked gelatin methacrylate (GelMa) spherical microgels. Spherical microgels were generated in a microfluidic system consisting of a co-axial flow-focusing device for microdroplet generation and an ultraviolet (UV) irradiation apparatus for polymerization. At a low flow rate ratio ( < 0.14), the 9 wt.% GelMa spherical microgels were smaller than the 6 wt.% ones. In contrast, at a high flow rate ratio ( > 0.14), the results were reversed. Overall, a proportional relationship was observed between the flow rate ratio and the droplet size. The increased GelMa concentration improved the mechanical properties and increased the swelling ratios. The possibility of bioencapsulation was demonstrated, with good viability of 3T3 cells encapsulated in the spherical microgels.

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“…Polyglycerol has a biocompatible and flexible polyether backbone with a high number of hydrophilic functional groups, which increases polyglycerol versatility and enables the production of hydrogels [14]. Citric acid is a relatively small and multifunctional monomer with pendant functional groups that allow future ester bond-crosslink and hydrogen bonding [15,16]. Fatty acids are relative long monomers with a carboxylic functional group that with chemical modification of doubledouble bond of unsaturated fatty acids allows the formation of crosslinked polymer structures [17].…”

Section: Introductionmentioning

confidence: 99%

Stimuli-Responsive Hydrogels Based on Polyglycerol Crosslinked with Citric and Fatty Acids

Solano-Delgado

Bravo-Sanabria

Ardila-Suárez

et al. 2018

International Journal of Polymer Science

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Polyglycerol-based hydrogels from biodegradable raw materials were synthesized by crosslinking reactions of polyglycerol with citric and fatty acids. Three hydrogels were studied varying molar ratios of crosslinking agent. It was found that crosslink amount, type, and size play a crucial role in swelling, thermal, mechanical, and stimuli-responsive properties. The hydrogels absorption capacity changed in response to temperature and pH external stimuli. The hydrogel with the highest swelling capacity absorbed more than 7 times its own weight at room temperature and pH 5. This material increased 14 times its own weight at pH 10. Creep-recovery tests were performed to study the effect of crosslinking agent on mechanical properties. Deformation and percentage of recovery of synthesized hydrogels were obtained. Formation of hydrogels was confirmed using FTIR, and physicochemical properties were analyzed by Scanning Electron Microscopy (SEM), Differential Scanning Calorimetric (DSC), and Dynamic Mechanical Analysis (DMA). This paper aims to give a contribution to biobased hydrogel knowledge from chemical, physicochemical, and mechanical point of view.

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Citric acid-derived in situ crosslinkable biodegradable polymers for cell delivery

Cited by 135 publications

References 45 publications

Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Increased paclitaxel cytotoxicity against cancer cell lines using a novel functionalized carbon nanotube

Swelling characterization of photo-cross-linked gelatin methacrylate spherical microgels for bioencapsulation

Stimuli-Responsive Hydrogels Based on Polyglycerol Crosslinked with Citric and Fatty Acids

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