Citric Acid Production from Sugarcane Bagasse through Solid State Fermentation Method Using Aspergillus niger Mold and Optimization of Citric Acid Production by Taguchi Method

Abstract

Background: Citric acid is produced in insignificant quantities in Iran. Despite its great range of utilizations, and from another aspect, high level of production of sugarcane bagasse, the related problems arising from maintenance of this acid require thinking of a measure in the direction of its optimal usage and production. Objectives: The objective of the present study is to obtain effectual variables in producing citric acid from sugarcane bagasse through Solid State Fermentation (SSF) method using Aspergillus niger mold and to optimize its mass production by employing Taguchi method. Materials and Methods:The effective parameters such as spore inoculation level, methanol percentage, solvent type, spore age, humidity percentage, initial pH of substrate, fermentation period and temperature, initial sugar percentage, autoclaving duration, nitrogen source and etc. were studied for producing citric acid from sugarcane bagasse with respect to Tagouchi method. Results: By considering the findings obtained from the tests, the highest production rate of citric acid g/kg out of untreated sugarcane bagasse is 75.45 based on the consumed sugar and a yield of 15.1 g/kg of sugarcane was achieved per day. Application of sodium hydroxide and acid pretreated sugarcane bagasse increased the production of citric acid in such a fashion that the production rates were 97.81 g/kg and 87.32 g/kg of sugarcane bagasse, respectively, compared to sodium hydroxide and acid untreated sugarcane bagasse. Conclusions:The obtained findings in the present study indicated that sugarcane bagasse is an ideal substrate in producing citric acid and the aforementioned process could be considered as a beneficial and cost-effective method in citric acid production.

A New Endophytic Fusarium Oxysporum Gibberellic Acid: Optimization of Production Using Combined Strategies of Experimental Designs and Potency on Tomato Growth under Stress Condition

“…It is well documented that solid state fermentation processes are largely influenced by the nature of the solid substrate. The substrates are generally water insoluble polymers of cellulosic or starchy material [25]. Our study indicated that GA3 production was increased by the addition of different compounds as inducers to the media.…”

ProducingAspergillus tubingensisCTM507 Glucose oxidase by Solid state fermentation versus submerged fermentation: process optimization and enzyme stability by an intermediary metabolite in relation with diauxic growth

“…Solid state fermentation processes are largely influenced by the nature of the solid substrate. The substrates are generally water insoluble polymers of cellulosic or starchy material . The selection of a suitable solid substrate for a fermentation process is a critical factor and thus involves the screening of a number of agro‐industrial materials for microbial growth and product formation .…”

“…The substrates are generally water insoluble polymers of cellulosic or starchy material. 53 The selection of a suitable solid substrate for a fermentation process is a critical factor and thus involves the screening of a number of agro-industrial materials for microbial growth and product formation. 33 Our study revealed that glucose oxidase activity was increased by the addition to the media of different compounds as inducers.…”

Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review

“…Apart from fruit wastes, citric acid can also be produced from agro-industrial wastes such as oil palm empty fruit bunches [142], sugarcane bagasse [143], cassava bagasse [144], and biodiesel by-products [145] through SSF. Yadegary et al [143] optimized citric acid production using sugarcane bagasse as a substrate by SSF using the Taguchi statistical method.…”

“…Yadegary et al [143] optimized citric acid production using sugarcane bagasse as a substrate by SSF using the Taguchi statistical method. Alkaline and acid pretreatment showed an increase in the yield production of 97.81 g·kg −1 and 87.32 g·kg −1 respectively, compared with untreated sugarcane bagasse yield of 75.45 g·kg −1 .…”