Citrobacter freundii infection in red swamp crayfish (Procambarus clarkii) and host immune-related gene expression profiles

Liu, Xiaodan; He, Xiaohong; An, Zhenhua; Sun, Wei; Chen, Nan; Gao, Xiaojian; Li, Xixi; Zhang, Xiaojun

doi:10.1016/j.aquaculture.2019.734499

Cited by 24 publications

(18 citation statements)

References 40 publications

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“…P. clarkii is omnivorous, feeding mainly on zooplankton during the juvenile period and on large aquatic plants during the adult period ( Li, 2006 ), which may be related to the large increase of Dysgonomonas at the adult stage. Citrobacter is a typical conditional pathogen widely found in nature, and its infection in P. clarkii activates the natural immune response of P. clarkii ( Liu et al, 2020 ); however, in general, Citrobacter is normally dominant in the intestine of crayfish ( Wu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Xie

Zhang²,

et al. 2021

Front. Microbiol.

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This study aimed to determine the effect of the growth stage of Procambarus clarkii on their intestinal microbiota. Intestinal samples of five different growth stages of P. clarkii (first instar, second instar, third instar, juvenile, and adult) from laboratory culture were analyzed through the Illumina MiSeq high-throughput sequencing platform to determine the intestinal microbiome of crayfish. The alpha diversity decreased along with the growth of the crayfish, with the relative abundance of the microbiota changing among stages; crayfish at closer development stages had a more comparable intestinal microbiota composition. A comparative analysis by principal component analysis and principal coordinate analysis showed that there were significant differences in the intestinal microbiota of crayfish among the different growth stages, except for the first two stages of larval crayfish, and the intestinal microbiota showed a consistent progression pattern from the larval stage to the juvenile stage. Some microbiota showed stage specificity, which might be the characteristic microbiota of different stages of growth. According to FAPROTAX functional clustering analysis, the three stages of larvae were clustered together, while the juvenile and adult stages were clustered separately according to the growth stage, indicating that, in the early stages of larval development, the function of the intestinal flora was similar; as the body grew and developed, the composition and function of the intestinal microbiota also changed.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Xie

Zhang²,

et al. 2021

Front. Microbiol.

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“…[6]. A challenge test showed that C. freundii exhibited significant virulence to crayfish, with an LD50 value of 1.71 × 10 6 CFU/mL [6]. In this study, after a 10-day challenge with a higher concentration (2.5 × 10 7 CFU/mL) of C. freundii, the cumulative survival rate was 30-40% in the control group.…”

Section: Disease Resistance Analysismentioning

confidence: 46%

“…Bacteroides could result in damage to intestinal tissues [58]. Citrobacter is an opportunistic bacterium in crayfish culture [6]. Although not statistically different, our results show that the relative abundances of Cetobacterium and Lactobacillus in the crayfish intestines from treatment group increased, and those of Citrobacter and Bacteroides decreased after feeding with S. cerevisiae for 14 days and 28 days.…”

Section: Intestinal Microbiota Analysismentioning

confidence: 52%

“…freundii can invade shrimp and produce independent factors leading to disease outbreaks. [6]. A challenge test showed that C. freundii exhibited significant virulence to crayfish, with an LD50 value of 1.71 × 10 6 CFU/mL [6].…”

Section: Disease Resistance Analysismentioning

confidence: 99%

“…C. freundii, as a typical conditional pathogen, has strong pathogenicity and lethality toward crayfish. This may result in economic losses to the crayfish aquaculture industry [6]. Many crayfish diseases are closely related to intestinal health, especially the structure of the intestinal microbiota [7].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Dietary Saccharomyces cerevisiae YFI-SC2 on the Growth Performance, Intestinal Morphology, Immune Parameters, Intestinal Microbiota, and Disease Resistance of Crayfish (Procambarus clarkia)

Xue

et al. 2021

Animals

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The present study aimed to evaluate the effect of the dietary supplementation of Saccharomyces cerevisiae YFI-SC2 on the growth performance, intestinal morphology, immune parameters, intestinal microbiota, and disease resistance of crayfish (Procambarus clarkia). Crayfish were randomly assigned to six different boxes and two different groups in triplicate. The control group received a basal diet and the treatment group received a diet containing S. cerevisiae at 107 CFU/g. After feeding for 28 days, crayfish of the treatment group exhibited a significantly better weight gain ratio (WGR) and a specific growth rate (SGR) (p < 0.05) than crayfish of the control group. Compared to the treatment group, the control group intestines showed an oedema connective tissue layer and a weak muscle layer. For immune-related genes, Crustin2 expression was similar between the groups, whereas Lysozyme and prophenoloxidase from treatment group expression levels were upregulated significantly (p < 0.05) after 14 and 28 days of feeding. Prophenoloxidase showed the highest expression, with 10.5- and 8.2-fold higher expression than in the control group at 14 and 28 days, respectively. The intestinal microbiota community structure was markedly different between the two groups. After 14 and 28 days of feeding, the relative abundance of Cetobacterium and Lactobacillus increased, whereas Citrobacter and Bacteroides decreased in the treatment group compared with that of the control group. The challenge test showed that crayfish of the treatment group had a significantly enhanced resistance against Citrobacter freundii (p < 0.05). Our results suggest that a S. cerevisiae-containing diet positively influenced the health status, immune parameters, intestinal microbiota composition, and disease resistance of crayfish.

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16S rRNA gene sequencing analysis on changes in the intestinal flora of Procambarus clarkii with “Black May” disease

et al. 2022

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Citrobacter freundii infection in red swamp crayfish (Procambarus clarkii) and host immune-related gene expression profiles

Cited by 24 publications

References 40 publications

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Effects of Dietary Saccharomyces cerevisiae YFI-SC2 on the Growth Performance, Intestinal Morphology, Immune Parameters, Intestinal Microbiota, and Disease Resistance of Crayfish (Procambarus clarkia)

16S rRNA gene sequencing analysis on changes in the intestinal flora of Procambarus clarkii with “Black May” disease

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