Citrobacter rodentium(ϕStx2dact), a murine infection model for enterohemorrhagic Escherichia coli

Thorpe, Cheleste M.; Pulsifer, Amanda R.; Osburne, Marcia S.; Leong, John M.

doi:10.1016/j.mib.2021.11.013

Cited by 9 publications

(6 citation statements)

References 63 publications

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“…Thus, they synthesized and evaluated the antimicrobial activity of these peptides corresponding to the N-terminal part of RP uL1, of which, only the peptide called Hp (2–20), containing only the first α-helix of the two amphipathic α-helices, showed antimicrobial activity against E. coli and Bacillus megaterium . In the case of E. coli , some strains were enterohemorrhagic, indicating a food-borne pathogen that causes bloody diarrhea and, in some individuals, life-threatening hemolytic uremic syndrome (HUS) [ 68 ]. Furthermore, the research by Pütsep et al [ 53 ] has been used in recent years as a basis for the synthesis of RPs with antimicrobial activity, due to the homology between RPs of different organisms.…”

Section: Moonlighting Proteinsmentioning

confidence: 99%

Ribosomes: The New Role of Ribosomal Proteins as Natural Antimicrobials

Hurtado-Rios

Carrasco-Navarro

Almanza-Perez

et al. 2022

IJMS

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Moonlighting proteins are those capable of performing more than one biochemical or biophysical function within the same polypeptide chain. They have been a recent focus of research due to their potential applications in the health, pharmacological, and nutritional sciences. Among them, some ribosomal proteins involved in assembly and protein translation have also shown other functionalities, including inhibiting infectious bacteria, viruses, parasites, fungi, and tumor cells. Therefore, they may be considered antimicrobial peptides (AMPs). However, information regarding the mechanism of action of ribosomal proteins as AMPs is not yet fully understood. Researchers have suggested that the antimicrobial activity of ribosomal proteins may be associated with an increase in intracellular reactive oxidative species (ROS) in target cells, which, in turn, could affect membrane integrity and cause their inactivation and death. Moreover, the global overuse of antibiotics has resulted in an increase in pathogenic bacteria resistant to common antibiotics. Therefore, AMPs such as ribosomal proteins may have potential applications in the pharmaceutical and food industries in the place of antibiotics. This article provides an overview of the potential roles of ribosomes and AMP ribosomal proteins in conjunction with their potential applications.

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Section: Moonlighting Proteinsmentioning

confidence: 99%

Ribosomes: The New Role of Ribosomal Proteins as Natural Antimicrobials

Hurtado-Rios

Carrasco-Navarro

Almanza-Perez

et al. 2022

IJMS

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“…GI infections in mice caused by Citrobacter rodentium are a model for infections with EHEC, which colonizes the intestinal epithelium by forming attaching and effacing (A/E) lesions (Higgins et al, 1999; Thorpe et al, 2022). The infection induces transient colitis that is resolved after 14 days in WT C57Bl/6 mice (Collins et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Interleukin 15 in murine models of colitis

Kautzman

Mobulakani

Cofino

et al. 2022

The Anatomical Record

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Inflammatory bowel diseases (IBDs) are characterized by abnormal, non-antigen specific chronic inflammation of unknown etiology. Genome-wide association studies show that many IBD genetic susceptibility loci map to immune function genes and compelling evidence indicate that environmental factors play a critical role in IBD pathogenesis. Clinical and experimental evidence implicate the pro-inflammatory cytokine IL-15 in the pathogenesis of IBD. IL-15 and IL-15α expression is increased in the inflamed mucosa of IBD patients. IL-15 contributes to the maintenance of different cell subsets in the intestinal mucosa. However, very few studies have addressed the role of IL-15 in pre-clinical models of colitis. In this study, we use three well-characterized models of experimental colitis to determine the contribution of IL-15 to pathological intestinal inflammation.

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“…This absence of a marked increase in IL-8 suggests that the response to EHEC infections in dogs may mirror the generally milder course of EHEC infections seen in dogs under natural conditions. A notable model for studying EHEC infections in humans is the murine model of Citrobacter rodentium infection (33, 34). Similar to what we observed in our study, TNF-α levels increase in the gut for this model.…”

Section: Discussionmentioning

confidence: 99%

Enterohemorrhagic Escherichia coli (EHEC) Disrupts Intestinal Barrier Integrity in Translational Canine Stem Cell-Derived Monolayers

Nagao,

Kawasaki,

Goyama

et al. 2024

Preprint

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This study addresses the gap in translatable in vitro models for investigating Enterohemorrhagic E. coli (EHEC) infections, particularly relevant to both canine and human health. EHEC is known to induce acute colitis in dogs, leading to symptoms like hemorrhagic diarrhea and hemolytic uremic syndrome, similar to those observed in humans. However, understanding the pathophysiology and developing treatment strategies have been challenging due to the lack of effective models that replicate the clinical disease caused by EHEC in both species. Our approach involved the development of colonoid-derived monolayers using intestinal tissues from healthy, client-owned dogs. These monolayers were exposed to EHEC and the impact of EHEC was assessed through several techniques, including trans-epithelial electrical resistance (TEER) measurement, immunofluorescence staining for junction proteins and mucus, and scanning electron microscopy for morphological analysis. Modified culture with saline, which was intended to prevent bacterial overgrowth, maintained barrier integrity and cell differentiation. EHEC infection led to significant decreases in TEER and ZO-1 expression, but not in E-cadherin levels or mucus production. Additionally, EHEC elicited a notable increase in TNF-α production, highlighting its distinct impact on canine intestinal epithelial cells compared to non-pathogenic E. coli. These findings closely replicate in vivo observations in dogs and humans with EHEC enteropathy, validating the canine colonoid-derived monolayer system as a translational model to study host-pathogen interactions in EHEC and potentially other clinically significant enteric pathogens.

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Citrobacter rodentium(ϕStx2dact), a murine infection model for enterohemorrhagic Escherichia coli

Cited by 9 publications

References 63 publications

Ribosomes: The New Role of Ribosomal Proteins as Natural Antimicrobials

Ribosomes: The New Role of Ribosomal Proteins as Natural Antimicrobials

Interleukin 15 in murine models of colitis

Enterohemorrhagic Escherichia coli (EHEC) Disrupts Intestinal Barrier Integrity in Translational Canine Stem Cell-Derived Monolayers

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