Citroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member

Abstract

A novel Gram-stain-positive, non-motile, non-spore-forming coccus-shaped obligately anaerobic bacterium was recovered from a fecal sample obtained from an individual from a traditional community located on the southern coast of Peru. The results of analysis based on 16S rRNA gene sequencing indicated the novel bacterium to be phylogenetically distinct from other genera of members of the Peptoniphilaceae family, sharing a loose affinity with the genera Ezakiella, Finegoldia, Gallicola and Parvimonas. The major cellular fatty acids of the novel isolate were determined to be C 16:0 , C 17:1 !8c, and C 18:1 !9c. The DNA G+C content was 29.9 mol%. End products of metabolism from peptone yeast glucose broth (PYG) were determined to be acetate and methyl succinate. The diagnostic diamino acid present in the cell wall was lysine. On the basis of the phenotypic, chemotaxonomic and phylogenetic results the organism is a member of a novel genus belonging to the family Peptoniphilaceae for which the name Citroniella saccharovorans gen nov. sp. nov., is proposed. The type strain is M6.X9 T (DSM 29873 T =CCUG 66799 T).

Clostridium tanneri sp. nov., isolated from the faecal material of an alpaca

“…Minerals solution (A) contained (per 1000 ml); K 2 HPO 4 (3.0 g). Minerals solution (B) contained (per 1000 ml); KH 2 PO 4 (3.0 g), (NH 4 ) 2 SO 4 (6.0 g), NaCl (6.0 g), MgSO 4 ×7H 2 O (0.6 g) and CaCl 2 (0.6 g) as described previously [11]. The enrichment was incubated at 37 °C for 14 days with a headspace of 5 % hydrogen, 10 % carbon dioxide and 85 % nitrogen to maintain anoxic conditions.…”

Complete genome sequence of Citroniella saccharovorans DSM 29873, isolated from human fecal sample

“…Citroniella saccharovorans (DSM 29873) was isolated from a fecal sample from a traditional native on the southern coast of Peru ( 1 ). C. saccharovorans grows under strict anaerobic conditions at optimum 30°C and pH 6.5–7.3, utilizing different sugars, organic acids, and amino acids for growth, with production of acetate and methyl succinate ( 1 ).…”

“…Citroniella saccharovorans (DSM 29873) was isolated from a fecal sample from a traditional native on the southern coast of Peru ( 1 ). C. saccharovorans grows under strict anaerobic conditions at optimum 30°C and pH 6.5–7.3, utilizing different sugars, organic acids, and amino acids for growth, with production of acetate and methyl succinate ( 1 ). This complete genome will provide possibilities to recover information on genes and potential metabolic pathways used by this species, representing the only isolate of the genus Citroniella .…”

“…This complete genome will provide possibilities to recover information on genes and potential metabolic pathways used by this species, representing the only isolate of the genus Citroniella . This strain was initially enriched anaerobically by inoculation of a fecal sample in liquid medium 2 ( 1 ). For isolation, aliquots of the enrichment were transferred to solid agar plates and colonies were finally picked and sub-cultured on the same medium.…”

Parvimonas parva sp. nov., derived from a human genito-urinary lesion

“…Incertae sedis in Bergey's Manual until the family Peptoniphilaceae was described in 2014 to include the genera Anaerococcus, Anaerosphaera, Finegoldia, Gallicola, Helcococcus, Murdochiella, Parvimonas and Peptoniphilus [1]. Since then, two additional novel validly published genera were assigned to this family (Citroniella and Ezakiella) [2,3]. Most isolates of Parvimonas (P.) micra exhibit strong proteolytic activity with possible significance in the development of mixed extraoral anaerobic abscesses [4,5].…”