Citrus flavanone metabolites protect pancreatic β-cells against cholesterol stress through a multi-proteomic mechanism

Abstract

Citrus flavanones may improve oxidative stress and insulin resistance induced by western diets.

Plasma proteome profiling reveals molecular mechanisms underlying the effects of daily consumption of ‘Bahia’ and ‘Cara Cara’ orange juices

“…59 NF-κB1 is a member of the NF-κB family which plays an important role in cell function and inflammation. Fraga et al 22 also found NF-κB1 significantly enriched after treatment of pancreatic β-cells in high concentrations of cholesterol with H7G, evidencing the hesperidin-mediated protection of pancreatic β-cells from apoptosis. This analysis suggests that OJ consumption could modulate the expression of plasma TFs that play a crucial role in reducing risks of CVD and other metabolic disorders.…”

“…48,49 Considering that flavanone and carotenoid metabolites can modulate the expression of large numbers of genes to exert their biological effects, we performed an untargeted proteomic analysis and observed, for the first time, OJ exerting important modifications on the plasma proteome. Although the effects of OJ consumption have been previously reported in PBMC 21 and pancreatic β-cells, 22,23 studies have not yet assessed the plasma proteome.…”

“…21 Most recently, we showed that flavanone-derived metabolites can prevent dysfunction of pancreatic β-cells under glucose-and cholesterol-induced stress through modulating the expression of several proteins. 22,23 Although the effects of OJ consumption have been reported in cell lines, some unknown mechanisms can only be visualized in plasma samples. Plasma proteome plays a central role in mediating the physiological response to nutritional changes, including proteins being transported between tissues (e.g., adipose tissue, liver, and kidney), binding-proteins, transporters, coagulation factors, and immune system proteins.…”