Citrus sinensis Essential Oils an Innovative Antioxidant and Antipathogenic Dual Strategy in Food Preservation against Spoliage Bacteria

Abstract

The present study evaluates the chemical compositions and antioxidant and antipathogenic properties of commercial orange (Citrus sinensis (L.) Osbeck) essential oils obtained using the cold-press method (EOP) and the cold-press method followed by steam distillation (EOPD). The chemical compositions of the volatilizable fractions, determined by gas chromatography-mass spectrometry, were similar in both samples. A relatively large amount of γ-terpinene was found in the EOPD (1.75%) as compared to the EOP (0.84%). Monoterpene hydrocarbons with limonene (90.4–89.8%) followed by myrcene (3.2–3.1%) as the main compounds comprised the principal phytochemical group. The non-volatile phenolics were eight times higher in the EOP than in the EOPD. Several assays with different specificity levels were used to study the antioxidant activity. Although both essential oils presented similar reducing capacities, the radical elimination ability was higher for the EOP. Regarding the antipathogenic properties, the EOs inhibited the biomass and cell viability of Staphylococcus aureus and Pseudomonas aeruginosa biofilms. Furthermore, both EOs similarly attenuated the production of elastase, pyocyanin, and quorum-sensing autoinducers as assessed using Gram-negative bacteria. The EOP and EOPD showed important antioxidant and antipathogenic properties, so they could represent natural alternatives to extend the shelf life of food products by preventing oxidation and contamination caused by microbial spoilage.

Preliminary Study on Insecticidal Potential and Chemical Composition of Five Rutaceae Essential Oils against Thrips flavus (Thysanoptera: Thripidae)

“…As we emphasized the control efficacy of the EOs against T. flavus , only a preliminary chemical analysis of the EOs was performed ( Figure S1 ). The chemical composition of the tested EOs was, in part, consistent with previous reports [ 30 , 47 , 48 , 49 ]. Limonene is the major constituent of EOs extracted from citrus by-products, whereas linalool, myrcene, sabinene, and pinene are characteristic of the Rutaceae EOs [ 48 , 49 , 50 ].…”

“…The chemical composition of the tested EOs was, in part, consistent with previous reports [ 30 , 47 , 48 , 49 ]. Limonene is the major constituent of EOs extracted from citrus by-products, whereas linalool, myrcene, sabinene, and pinene are characteristic of the Rutaceae EOs [ 48 , 49 , 50 ]. However, certain EOs’ constituents differed from those reported in other studies.…”

“…However, certain EOs’ constituents differed from those reported in other studies. For example, the analysis of essential oil from the orange peel parts of C. sinensis from Argentina identified limonene (90.48%) as the most prominent compound [ 49 ]. The main constituents of Zanthoxylum bungeanum essential oil, extracted using a low-eutectic solvent and steam distillation, were terpineol-4-ol (13.13%) and (–)-β-pinene (11.17%) [ 51 ].…”

Effects of the protective and therapeutic effect of yogurt enriched with Citrus sinensis essential oil against ibuprofen‐induced gastric ulcer in elderly rats

“…Citrus flavors are widely used in the food industry (Abd El‐aal and Halaweish, 2010; Mahato et al., 2019; Manzur et al., 2023; Yerou et al., 2017). The citral, limonene, and linalool found in orange EO are generally recognized as safe (Fisher & Philips, 2006).…”

Chemical compositions and biological activities of Serevenia buxifolia essential oil leaves cultivated in Vietnam (Thua Thien Hue)

“…Hence, there is growing interest in their replacement with natural products (Hayet et al, 2017). One such possibility may be the use of herbal and botanical products like essential oils (EOs) and plant extracts exhibiting desired biological activities (Brah et al, 2023; Li et al, 2022; Manzur et al, 2023; Shen et al, 2022). For example, the potent antibacterial (Cazella et al, 2019; Ghavam et al, 2020; Man et al, 2019), antifungal (Gakuubi et al, 2017; Nazzaro et al, 2017), insecticidal (Kweka et al, 2011; Owolabi et al, 2014), acaricidal (Djebir et al, 2019), cytotoxic (Khang et al, 2020; Russo et al, 2015), antioxidant (Cardoso‐Ugarte & Sosa‐Morales, 2021; Torres‐Martínez et al, 2018), and antidiabetic (Siahbalaei et al, 2020) properties of EOs have been investigated.…”