Civa Klorür Uygulanan Gökkuşağı Alabalıkları (Oncorhynchus Mykiss)’ nın Solungaç Dokusunda Nitrozatif ve Oksidatif Stresin Rolü

Abstract

The aim of the study was to determine the toxic effect and the dynamics of oxidative stress caused by mercury chloride (HgCl2) in rainbow trout (Oncorhynchus mykiss) weighing 59.43±7.21 g.For this purpose, a total of 40 fish in 4 groups, 10 fish in each group (n=10), were exposed to 25% and 50% (137.75 μg L and 275 μg L) of the LD50 for 2 and 7 days. To determine the oxidative/nitrosative stress, Peroxynitrite (ONOOˉ mmol L), Total Oxidant Level (TOS mmol H2O2 Eq L), Total Antioxidant Level (TAS mmol Trolox Eq L), OSI (TOS/TASx10) and Malondialdehyde (MDA) level were determined. While the difference between the groups in terms of TAS, OSI and MDA levels in gill tissues was statistically significant (P<0.05), this difference was statistically insignificant (P>0.05) in terms of ONOOˉ and TOS values. As a result, HgCl2 was found to cause stress and toxic to fish as it increases the levels of ONOOˉ, OSI and MDA, which are indicators of stress in gill tissue.