Clarithromycin resistance and point mutations in the 23S rRNA gene in <i>Helicobacter pylori</i> isolates from Malaysia

Ho, Sin-Loong; Tan, Eng Lai; Sam, Chook Koon; Goh, Khean‐Lee

doi:10.1111/j.1751-2980.2010.00423.x

Cited by 28 publications

(32 citation statements)

References 30 publications

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“…These results were consistent with data obtained from other authors from studies conducted in Iran in 2011 (the rate of clarithromycin resistance was 31.7%, all of them had A2143G mutation detected using PCR-RFLP) [16] and more recently in USA in 2016 (clarithromycinresistant H. pylori was common and observed in 32.3 % of cases; the specific variants A2142G and A2143G were detected using DNA sequencing) [17]. However, the rates of A2142G and A2143G mutation in our study were significantly higher than those reported from a study conducted in Malaysia in 2010 (only one sample with A2142G mutation and two samples with A2143G mutation were found among 105 samples, accounted for only 2.9%) [18], but significantly lower than the one in Yamade's study conducted in Japan (the mutation rate was 55.6% including 82 samples with A2143G mutation and 3 samples with A2142G) [19].…”

Section: Discussioncontrasting

confidence: 75%

Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam

Tran

et al. 2018

J Infect Dev Ctries

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Introduction: Data about the prevalence of the A2142C, A2142G, and A2143G mutations in 23S rRNA gene is still limited. The aim of this study was to determine the prevalence of these mutations in 23S rRNA gene of H. pylori vietnamese strains. Methodology: One hundred and sixty-nine patients with H. pylori-positive chronic gastritis were examined. H. pylori was detected by rapid urease test and Polymerase chain reaction (PCR). Total DNA was extracted from gastric biopsy specimens. A2142C, A2142G, and A2143G mutations were detected by DNA sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP). Results: A2143G mutation was detected in 36.1% of samples, A2142G mutation in 3.6%, while A2142C mutation was not found in any case. The mixture of wild-type and mutation strains was found in 50% of specimens with A2142G, in 23% of specimens with A2143G mutation. There was no association of 23S rRNA gene point mutations with gender or age. However, an association between the heterogeneity of mutation and age was evidenced, with mean age of the group of pure A2143G higher than the group of wild-type/A2143G mixture, and rate of the wildtype/A2143G mixture higher in patients under 40 years of age. Conclusion: A2143G mutation was prominent, while A2142C mutation was not found in the 23S rRNA gene. PCR-RFLP has revealed a reliable assay allowing a rapid and cost-effective detection of clarithromycin-resistant strains. This is useful in countries as Vietnam with high prevalence of clarithromycin-resistance before choosing optimal therapy for H. pylori eradication.

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Section: Discussioncontrasting

confidence: 75%

Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam

Tran

et al. 2018

J Infect Dev Ctries

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“…Also, various results have been reported in other countries. In a study in Malaysia, the prevalence of the point mutation A2143G among 105 H. pylori strains was 1.9% (2/105), which is close to its prevalence in our work (6). In a study from Turkey, of 37 H. pylori-positive specimens, four (10.8%) had the A2143G mutation and 11 (29.7%) had the A2144G mutation.…”

Section: Discussionmentioning

confidence: 80%

“…It is understood from the foregoing articles that the prevalence of the point mutations vary widely from region to region. The reasons for these different results are difficult to explain, but may be due to differences in the prescription and administration of clarithromycin (6,21). In fact, the primary risk factor for resistance to clarithromycin is the previous use of clarithromycin or other macrolides (20).…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to clarithromycin in H. pylori is caused predominantly by point mutations in the peptidyl transferase center of 23S rRNA (5,8,9). These mutations prevent the binding of clarithromycin to the 50S subunit of the ribosome (6). It has been proven that fluorescent in situ hybridization (FISH) by the use of rRNA-targeted oligonucleotide probes is an accurate and convenient molecular technique for the detection of three 23S rRNA point mutations responsible for clarithromycin resistance, in which the adenine residues at positions 2143 and 2144 are replaced by guanine (A2143G and A2144G) or cytosine (A2143C) (8,10,11).…”

Section: Introductionmentioning

confidence: 99%

“…Clarithromycin is the most effective antimicrobial used for the treatment of H. pylori infection, and resistance to this drug is the major cause of treatment failure (3)(4)(5). The prevalence of clarithromycin resistance may vary in different geographical regions, and continuous monitoring of the clarithromycin resistance rate in each region is important for designing the most effective therapies (6,7).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of A2143G and A2144G Point Mutations Responsible for Clarithromycin Resistance among Helicobacter pylori Strains in Bushehr, Iran

Tajbakhsh

Falahi²,

Motamed³

et al. 2016

Avicenna J Clin Microbiol Infect

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Background: Resistance to clarithromycin in Helicobacter pylori has become one of the most important reasons for failure of antibiotic eradication therapies. This resistance is predominantly caused by point mutations in the peptidyl transferase region of 23S rRNA. Objectives: The aim of this study was to determine the prevalence of the A2143G, A2144G, and A2143C point mutations among H. pylori strains from gastric biopsy specimens in Bushehr, in the southwest of Iran. Patients and Methods: Gastric biopsy specimens were obtained from patients with upper gastrointestinal symptoms during endoscopy. Fluorescent in situ hybridization (FISH) using oligonucleotide probes was applied to detect the point mutations responsible for clarithromycin resistance in H. pylori. Results: Of the 135 H. pylori-positive specimens, two harbored strains with the A2143G mutation and nine contained strains with the A2144G mutation. Thus, the prevalences of the A2143G and A2144G point mutations were 1.5% and 6.7%, respectively. The A2143C point mutation was not found. Conclusions:The prevalences of the point mutations A2143G and A2144G were low in our geographic area. Based on the findings of this study, clarithromycin still seems to be a useful antibiotic for initial treatment regimens in Bushehr, Iran.

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Molecular detection of genotypic clarithromycin‐resistant strains and its effect on the eradication rate of concomitant therapy in Helicobacter pylori infection

2021

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Antimicrobial eradication rates for Helicobacter pylori have been decreasing and the reason for treatment failure was found to be resistance to one or more of the antibiotics. Clarithromycin resistance to H pylori was associated with point mutations in the 23S rRNA gene and the PCR-RFLP method can detect these point mutations. The aim of this study was to determine the molecular detection of genotypic clarithromycinresistant strains and its effect on the eradication rate of concomitant therapy in H pylori infection. The presence of H pylori DNA was confirmed by amplifying the UreC gene by polymerase chain reaction (PCR) and point mutations on 23S rRNA (A2142G and A2143G) were detected by PCR-RFLP. A total of 98 H pylori-infected patients were involved and among them, genotypic clarithromycin-sensitive strain was 93.9% and clarithromycin-resistant strain was 6.1%. All patients were found to have the A2143G point mutation but A2142G was not detected. Successful eradication rate of concomitant therapy was found to be 89.8% and unsuccessful rate was 10.2%.Among patients with the clarithromycin-resistant gene, only 16.7% had successful eradication and 83.3% had unsuccessful eradication. There was a statistically significant association between failure rate of concomitant therapy and detection of clarithromycin-resistant genes (P < 0.01). The presence of A2143G point mutation in the clarithromycin-resistant strain has a negative effect on the eradication rate of H pylori infection.How to cite this article: Nyi KN, Soe AM, Htut ZM. Molecular detection of genotypic clarithromycin-resistant strains and its effect on the eradication rate of concomitant therapy in

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Clarithromycin resistance and point mutations in the 23S rRNA gene in Helicobacter pylori isolates from Malaysia

Cited by 28 publications

References 30 publications

Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam

Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam

Prevalence of A2143G and A2144G Point Mutations Responsible for Clarithromycin Resistance among Helicobacter pylori Strains in Bushehr, Iran

Molecular detection of genotypic clarithromycin‐resistant strains and its effect on the eradication rate of concomitant therapy in Helicobacter pylori infection

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