2022
DOI: 10.5603/fm.a2021.0012
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CLARITY techniques based tissue clearing: types and differences

Abstract: This article has been peer reviewed and published immediately upon acceptance. It is an open access article, which means that it can be downloaded, printed, and distributed freely, provided the work is properly cited. Articles in "Folia Morphologica" are listed in PubMed.

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Cited by 4 publications
(3 citation statements)
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“…Since the purpose of CUBIC-R incubation is to homogenize the refractive index of the tissue, and is not directly involved in tissue clearing per se , CUBIC-R incubation may be omitted or substituted by another incubation medium that could homogenize refractive index and is compatible with FJ-C. Although hydrogel-embedded tissue-clearing techniques have emerged with a few variations, 39 the core components remain the same, with differences in terms of (1) concentrations or ratios of chemical compounds, (2) whether using electrophoretic forces (active vs passive), (3) incubation times, and (4) incubation temperatures. Therefore, this study tested only one of them, pClarity, and the negative outcome of the pClarity test suggests that FJ-C may not be compatible with the incubation media of hydrogel-embedded tissue-clearing methods.…”
Section: Discussionmentioning
confidence: 99%
“…Since the purpose of CUBIC-R incubation is to homogenize the refractive index of the tissue, and is not directly involved in tissue clearing per se , CUBIC-R incubation may be omitted or substituted by another incubation medium that could homogenize refractive index and is compatible with FJ-C. Although hydrogel-embedded tissue-clearing techniques have emerged with a few variations, 39 the core components remain the same, with differences in terms of (1) concentrations or ratios of chemical compounds, (2) whether using electrophoretic forces (active vs passive), (3) incubation times, and (4) incubation temperatures. Therefore, this study tested only one of them, pClarity, and the negative outcome of the pClarity test suggests that FJ-C may not be compatible with the incubation media of hydrogel-embedded tissue-clearing methods.…”
Section: Discussionmentioning
confidence: 99%
“…To confirm the identified MN markers, the initial step uses immunohistochemistry, staining the identified MN potential markers (THEMIS, UBE2F) within multiple postmortem patients' cortical areas. These brain slices can be reconstituted into a brain-wide 3D circuitry map using experimental methods such as brain-wide reduced-osmium staining with pyrogallol-mediated amplification (BROPA) or clarity (which preserves only proteins, tissue structure, and nucleic acids in specimens) (59,60). Second, conditional knockout animal models, such as mice, can be developed using CRISPR/Cas9 genome editing technology, followed by tests for MN-like behavior.…”
Section: Discussionmentioning
confidence: 99%
“…Currently, hydrogel-based tissue-clearing methods [ 94 , 97 , 98 , 99 ] are becoming the most commonly used methods. They include ‘cleared lipid-extracted acryl-hybridized rigid immunostaining/in situ hybridization-compatible tissue hydrogel’ (CLARITY) [ 100 ] and ‘stabilization to harsh conditions via intramolecular epoxide linkages to prevent degradation’ (SHIELD) [ 101 ]. CLARITY and SHIELD allow for a uniform delipidation of the tissue, and due to the presence of the hydrogel, also the stabilization and preservation of a large assortment of cell biomolecules through covalent linkages [ 98 ].…”
Section: Microscopy Imaging Of the Brain In Obesitymentioning
confidence: 99%