Class-A scavenger receptor function and expression in the rainbow trout (Oncorhynchus mykiss) epithelial cell lines RTgutGC and RTgill-W1

Poynter, Sarah J.; Weleff, Jeremy; Soares, Adam B.; DeWitte‐Orr, Stephanie J.

doi:10.1016/j.fsi.2015.01.028

Cited by 26 publications

(26 citation statements)

References 34 publications

(58 reference statements)

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“…; Poynter et al . ). In the former study, titres of VHSV IVb obtained from RTgill‐W1 were less than those from EPC and RTgill‐W1 also differentially supported the replication of VHSV IVa over IVb.…”

Section: Discussionmentioning

confidence: 97%

“…More recently, expression of Mx1, 3 and IFN1 genes was induced in RTgill‐W1 by poly I:C exposure (Poynter et al . ). An antiviral state was demonstrable in the present study at low infective doses of VHSV; however, these doses are likely reflective of those that fish are exposed to naturally.…”

Section: Discussionmentioning

confidence: 97%

“…This was also seen in walleye experimentally infected with VHSV IVb (Grice et al 2014). Fish cell lines are indispensable tools to study innate immunity capability; however, the majority used are not of epithelial origin Milev-Milovanovic et al 2009), and only two studies and used a cell line derived from the gill epithelium (Pham et al 2013;Poynter et al 2015). In the former study, titres of VHSV IVb obtained from RTgill-W1 were less than those from EPC and RTgill-W1 also differentially supported the replication of VHSV IVa over IVb.…”

Section: The Gill Epithelium and Vhsv Ivbmentioning

confidence: 99%

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Temporary protection of rainbow trout gill epithelial cells from infection with viral haemorrhagic septicaemia virus IVb

Al-Hussinee

Pham

Russell

et al. 2016

Journal of Fish Diseases

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The branchial epithelium is not only a primary route of entry for viral pathogens, but is also a site of viral replication and subsequent shedding may also occur from the gill epithelium. This study investigated the potential of agents known to stimulate innate immunity to protect rainbow trout epithelial cells (RTgill-W1) from infection with VHSV IVb. RTgill-W1 cells were pretreated with poly I:C, FuGENE(®) HD + poly I:C, lipopolysaccharide (LPS), LPS + poly I:C or heat-killed VHSV IVb and then infected with VHSV IVb 4 days later. Cytopathic effect (CPE) was determined at 2, 3, 4, 7 and 11 days post-infection. Virus in cells and supernatant was detected using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). All of the treatments delayed the onset of CPE (per cent of monolayer destruction), compared with untreated controls; however, killed VHSV or poly I:C combined with LPS was the most effective. Similarly, the detection of viral RNA in the supernatant was delayed, and the quantity was significantly (P < 0.05) reduced by all treatments with the exception of LPS alone (4 days). Unlike many of the other treatments, pretreatment of RTgill-W1 with heat-killed VHSV did not upregulate interferon 1, 2 or MX 1 gene expression.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: The Gill Epithelium and Vhsv Ivbmentioning

confidence: 99%

See 1 more Smart Citation

Temporary protection of rainbow trout gill epithelial cells from infection with viral haemorrhagic septicaemia virus IVb

Al-Hussinee

Pham

Russell

et al. 2016

Journal of Fish Diseases

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“…; Poynter et al . ). Exogenous dsRNA treatment triggers IFNs, ISGs, and the antiviral state in fish, illustrating that fish likely possess many of the same dsRNA response pathways as mammals (DeWitte‐Orr et al .…”

mentioning

confidence: 97%

“…) and β‐actin 53 °C (Poynter et al . ). All PCR products were sequenced to confirm identity (data not shown).…”

mentioning

confidence: 97%

Fish viruses make dsRNA in fish cells: characterization of dsRNA production in rainbow trout (Oncorhynchus mykiss) cells infected with viral haemorrhagic septicaemia virus, chum salmon reovirus and frog virus 3

Doherty

Poynter

Aloufi

et al. 2016

Journal of Fish Diseases

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Class A scavenger receptor expression and function in eight novel tadpole cell lines from the green frog (Lithobates clamitans) and the wood frog (Lithobates sylvatica)

Everson

Moore

et al. 2019

Cytotechnology

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A total of eight tadpole cell lines were established from green frogs (Lithobates clamitans) and wood frogs (Lithobates sylvatica). The five green frog cell lines were named GreenTad-HF1, GreenTad-HF2, GreenTad-HF3, GreenTad-HE4, and GreenTadgill. The three wood frog cell lines were named WoodTad-HE1, WoodTad-Bone, and WoodTad-rpe. DNA barcoding confirmed the cell lines to be from the correct species and the growth characteristics (optimal temperature and FBS requirement) were elucidated. In order to begin studying the innate immune capacity for each cell line, class A scavenger receptor expression and function were next explored. All cell lines expressed genes for at least 3 of the 5 class A scavenger receptor (SR-A) family members, but the gene expression patterns varied between cell lines. MARCO was only expressed in GreenTad-HE4 and WoodTad-Bone, while only GreenTad-HF3 did not express SCARA5 and only WoodTad-rpe did not express SR-AI. Acetylated low density lipoprotein (AcLDL) is a well-defined ligand for SR-As and WoodTad-rpe was the only cell line to which it was unable to bind. In the other seven tadpole cell lines, the SR-A competitive ligands (dextran sulfate, fucoidan, polyinosinic acid) blocked AcLDL binding whereas the SR-A non-competitive ligand counterparts (chondroitin sulfate, fetuin, polycytidylic acid, respectively) did not. Overall, these new eight cell lines can become important tools in the study of innate immunity in general and SR-A functions in particular in green frogs and wood frogs.

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Class-A scavenger receptor function and expression in the rainbow trout (Oncorhynchus mykiss) epithelial cell lines RTgutGC and RTgill-W1

Cited by 26 publications

References 34 publications

Temporary protection of rainbow trout gill epithelial cells from infection with viral haemorrhagic septicaemia virus IVb

Temporary protection of rainbow trout gill epithelial cells from infection with viral haemorrhagic septicaemia virus IVb

Fish viruses make dsRNA in fish cells: characterization of dsRNA production in rainbow trout (Oncorhynchus mykiss) cells infected with viral haemorrhagic septicaemia virus, chum salmon reovirus and frog virus 3

Class A scavenger receptor expression and function in eight novel tadpole cell lines from the green frog (Lithobates clamitans) and the wood frog (Lithobates sylvatica)

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