Class A Scavenger Receptor Up-regulation in Smooth Muscle Cells by Oxidized Low Density Lipoprotein

Mietus‐Snyder, Michele; Gowri, Maya S.; Pitas, Robert E.

doi:10.1074/jbc.275.23.17661

Cited by 96 publications

(57 citation statements)

References 68 publications

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“…Atherosclerosis has been reported to start as fatty streak lesions which contain foam cells, mainly of macrophage origin (14). The urgency of elucidating the mechanisms by which macrophages become foam cells and help to drive this pathology is a persistent need often voiced in the literature in this research field.…”

Section: Resultsmentioning

confidence: 99%

“…But merely determining the expression level for several genes chosen in advance is not sufficient to actually characterize cells, since in response to a given stimulus genes are often expressed in several different organs. Taking SR-A for example, it has recently been reported that PMA stimulation also induced SR-A expression in smooth muscle cells (14), making detection of SR-A difficult to employ as a specific macrophage differentiation marker. Thus, a method to study many genes at once became necessary, and we decided to use oligonucleotide microarray analysis to characterize THP-1 cells and their differentiated form The number under monocyte, GM-CSF 3d, GM-CSF 7d, THP-1, THP1PMA 7d denotes the average difference of each gene.…”

Section: Resultsmentioning

confidence: 99%

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A Comparison of Differences in the Gene Expression Profiles of Phorbol 12-myristate 13-acetate Differentiated THP-1 Cells and Human Monocyte-derived Macrophage

Kohro

Tanaka

Murakami

et al. 2004

JAT

156

120

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In order to characterize the monocytic cell line THP-1 and its mature, macrophagelike form treated with phorbol 12-myristate 13-acetate (PMA), we have conducted an oligonucleotide microarray assay and compared the results with those from an assay of human monocytes and macrophages. We found that early THP-1 cells have a pattern of gene expression distinct from monocytes, and when treated with PMA, certain genes which are induced in macrophages, such as apolipoprotein-E, matrix metalloproteinase 9 and α2 macroglobulin are also induced in the PMA-treated THP-1 cells (THP1PMA cells). However, these were some genes which are conversely regulated among macrophages and THP1PMA cells such as interleukin-1-β and the overall correlation coefficient was not very high. It is shown that, although certain morphological and other characteristics of PMA-differentiated THP-1 cells are similar to macrophages, from a transcriptomic view, the two are different. This suggests a need for careful recognition of and allowance for this difference when interpreting the results of experiments done with THP-1 cells in which it is otherwise assumed they are representative of the macrophage. J Atheroscler Thromb, 2004; 11: 88-97.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Comparison of Differences in the Gene Expression Profiles of Phorbol 12-myristate 13-acetate Differentiated THP-1 Cells and Human Monocyte-derived Macrophage

Kohro

Tanaka

Murakami

et al. 2004

JAT

156

120

View full text Add to dashboard Cite

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“…These studies raised the question of why SRA1 is induced in VSMCs, and one possibility is that in the apoptotic environment of the VSMC nodule, oxidative stress would be expected, which is a known inducer of expression of SRA in VSMCs. 25 Treatment of nodules with PI inhibited acLDL-induced stimulation of calcification. Although PI is not a specific SRA ligand, these results suggest that acLDL may interact with SRA1 to stimulate calcification.…”

Section: Discussionmentioning

confidence: 99%

Acetylated Low-Density Lipoprotein Stimulates Human Vascular Smooth Muscle Cell Calcification by Promoting Osteoblastic Differentiation and Inhibiting Phagocytosis

et al. 2002

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“…In this study, the levels of CD36 mRNA and protein, both of which were expressed in a constitutive manner, did not change following UA treatment (Fig. 5A), while a previous study found that ligandbinding induced oxidative stress up-regulated SR gene expression, thereby promoting the uptake of oxLDL (44).…”

Section: Figurementioning

confidence: 77%

Aggregated Ursolic Acid, a Natural Triterpenoid, Induces IL-1β Release from Murine Peritoneal Macrophages: Role of CD36

Ikeda

Murakami

Fujimura

et al. 2007

The Journal of Immunology

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IL-1β has been shown to play a pivotal role in the development of inflammatory disorders. We recently found that a natural triterpene, ursolic acid (UA), enhanced MIF release from nonstimulated macrophages. In this study, we examined the effects of UA on the production of several cytokines in resident murine peritoneal macrophages (pMφ). UA increased the protein release of IL-1β, IL-6, and MIF, but not of TNF-α, in dose- and time-dependent manners. This triterpene also strikingly induced the activation of p38 MAPK and ERK1/2 together with that of upstream kinases. The release of UA-induced IL-1β was significantly inhibited by the inhibitors of p38 MAPK, MEK1/2, ATP-binding cassette transporter, and caspase-1. Furthermore, UA induced intracellular ROS generation for IL-1β production, which was suppressed by an antioxidant. Pretreatment with an anti-CD36 Ab significantly suppressed IL-1β release, and surface plasmon resonance assay results showed that UA bound to CD36 on macrophages. In addition, the amount of IL-1β released from UA-treated pMφ of CD36-deficient mice was markedly lower than that from those of wild-type mice. Interestingly, UA was found to aggregate in culture medium, and the aggregates were suggested to be responsible for IL-1β production. In addition, i.p. administration of UA increased the levels of IL-1β secretion and MPO activity in colonic mucosa of ICR mice. Taken together, our results indicate that aggregated UA is recognized, in part, by CD36 on macrophages for generating ROS, thereby activating p38 MAPK, ERK1/2, and caspase-1, as well as releasing IL-1β protein via the ATP-binding cassette transporter.

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Class A Scavenger Receptor Up-regulation in Smooth Muscle Cells by Oxidized Low Density Lipoprotein

Cited by 96 publications

References 68 publications

A Comparison of Differences in the Gene Expression Profiles of Phorbol 12-myristate 13-acetate Differentiated THP-1 Cells and Human Monocyte-derived Macrophage

A Comparison of Differences in the Gene Expression Profiles of Phorbol 12-myristate 13-acetate Differentiated THP-1 Cells and Human Monocyte-derived Macrophage

Acetylated Low-Density Lipoprotein Stimulates Human Vascular Smooth Muscle Cell Calcification by Promoting Osteoblastic Differentiation and Inhibiting Phagocytosis

Aggregated Ursolic Acid, a Natural Triterpenoid, Induces IL-1β Release from Murine Peritoneal Macrophages: Role of CD36

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