Class A scavenger receptors mediate extracellular dsRNA sensing, leading to downstream antiviral gene expression in a novel American toad cell line, BufoTad

Vo, Nguyen T. K.; Moore, Levi C.; Leis, Eric; DeWitte‐Orr, Stephanie J.

doi:10.1016/j.dci.2018.11.012

Cited by 16 publications

(14 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a slowly developing CPE was observed in BufoTad cells (Figure 2), associated with both KSL and IF samples on day 20 after the initial inoculation, 12 d after first reset, and 10 d after the second reset. Typical monolayers of the BufoTad cell line display a homogenous, cobblestone appearance with apparent contact inhibition (Vo et al 2019). The BufoTad monolayers inoculated with samples from the mudpuppy contained numerous detached cells and a loss of contact inhibition, which was especially interesting given that the samples were derived from a tumor.…”

Section: Resultsmentioning

confidence: 99%

“…Three cell lines, epithelioma papulosum cyprini (EPC), Chinook Salmon embryo (CHSE), and a recently developed cell line (BufoTad) derived from American toad Anaxyrus americanus tadpoles (Vo et al 2019) were used for pathogen isolation. All cells were grown to confluence in minimum essential medium with Hank's balanced salts (Sigma‐Aldrich) and maintained at 20°C.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Nephroblastoma in a Common Mudpuppy Necturus maculosus simultaneously Present with a Mollicute Bacterium of the Genus Acholeplasma

et al. 2021

Self Cite

View full text Add to dashboard Cite

In March 2017, a wild‐caught female common mudpuppy Necturus maculosus from Iowa, USA, with an enlarged posterior abdomen was submitted for diagnostic assessment. The cause of the abdominal distension was a large fluid‐filled abdominal mass, diagnosed as a nephroblastoma. Parasites and numerous bacteria were isolated and identified from the mudpuppy but were determined to be incidental. Samples of the neoplasm inoculated onto an American toad Anaxyrus americanus cell line (BufoTad) yielded cytopathic effect during several passages. However, standard molecular testing of the cell culture supernatant failed to identify any viruses. Next‐generation sequencing identified the replicating agent as a bacterium of the genus Acholeplasma. Immunohistochemistry confirmed the presence of Acholeplasma within the nephroblastoma, including within tumor cells. This is the first report of nephroblastoma and the second report of neoplasia in this species. The results also suggest that certain bacteria of the genus Acholeplasma might be oncogenic.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Nephroblastoma in a Common Mudpuppy Necturus maculosus simultaneously Present with a Mollicute Bacterium of the Genus Acholeplasma

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Conversely, the X. laevis CSF-1- and IL-34-derived Mϕs expressed MARCO, SCARA3, and SCARA4, but did not express SR-AI or SCARA5 (Table 2). All SR-A gene expression was determined by RT-PCR using validated primers [35,51] based on the species-specific partial/complete SR-A sequences in the National Center for Biotechnology Information (NCBI) database.…”

Section: Resultsmentioning

confidence: 99%

“…There are five known members in the SR-A family: SR-AI/II, macrophage receptor with collagenous domain (MARCO), scavenger receptor A (SCARA)3, SCARA4, and SCARA5 [29,30,31,32]. SR-As are present in all vertebrates and absent in invertebrates [33], ubiquitously expressed by many distinct cell types [27,29,32,34,35], function at a wide range of temperatures, exhibit evolutionarily conserved ligand binding properties [27,34,35,36,37], and facilitate clathrin/caveola-mediated endocytosis and macropinocytosis [38,39,40]. Moreover, several SR-A members facilitate cellular entry of DNA viruses.…”

Section: Introductionmentioning

confidence: 99%

Class A Scavenger Receptors Are Used by Frog Virus 3 During Its Cellular Entry

Guerreiro

Yaparla

et al. 2019

Viruses

View full text Add to dashboard Cite

Frog virus 3 (FV3) is the type species of the genus Ranavirus (family Iridoviridae). FV3 and FV3-like viruses are globally distributed infectious agents with the capacity to replicate in three vertebrate classes (teleosts, amphibians, and reptiles). At the cellular level, FV3 and FV3-like viruses can infect cells from virtually all vertebrate classes. To date, the cellular receptors that are involved in the FV3 entry process are unknown. Class A scavenger receptors (SR-As) are a family of evolutionarily conserved cell-surface receptors that bind a wide range of chemically distinct polyanionic ligands and can function as cellular receptors for other DNA viruses, including vaccinia virus and herpes simplex virus. The present study aimed to determine whether SR-As are involved in FV3 cellular entry. By using well-defined SR-A competitive and non-competitive ligand-blocking assays and absolute qPCR, we demonstrated that the SR-A competitive ligands drastically reduced the quantities of cell-associated viral loads in frog cells. Moreover, inducing the expression of a human SR-AI in an SR-A null cell line significantly increased FV3–cell association. Together, our results indicate that SR-As are utilized by FV3 during the cellular entry process.

show abstract

“…Interestingly, MARCO does not appear to bind dsRNA, a unique finding in vertebrates [59]. SR-As appear to be surface receptors in amphibian cells as well, as Dr. DeWitte-Orr presented her findings regarding SR-A mediated dsRNA responses in the American toad cell line, BufoTad [62]. In addition to function studies, the DeWitte-Orr group has identified a cell line derived from Chinook salmon (CHSE-214) that appears to lack functional SR-As and can be used as a model for SR-A/dsRNA binding [63].…”

Section: Class a Scavenger Receptors In Innate Antiviral Immunitymentioning

confidence: 99%

CSV2018: The 2nd Symposium of the Canadian Society for Virology

Grandvaux

McCormick

2019

Viruses

View full text Add to dashboard Cite

The 2nd Symposium of the Canadian Society for Virology (CSV2018) was held in June 2018 in Halifax, Nova Scotia, Canada, as a featured event marking the 200th anniversary of Dalhousie University. CSV2018 attracted 175 attendees from across Canada and around the world, more than double the number that attended the first CSV symposium two years earlier. CSV2018 provided a forum to discuss a wide range of topics in virology including human, veterinary, plant, and microbial pathogens. Invited keynote speakers included David Kelvin (Dalhousie University and Shantou University Medical College) who provided a historical perspective on influenza on the 100th anniversary of the 1918 pandemic; Sylvain Moineau (Université Laval) who described CRISPR-Cas systems and anti-CRISPR proteins in warfare between bacteriophages and their host microbes; and Kate O’Brien (then from Johns Hopkins University, now relocated to the World Health Organization where she is Director of Immunization, Vaccines and Biologicals), who discussed the underlying viral etiology for pneumonia in the developing world, and the evidence for respiratory syncytial virus (RSV) as a primary cause. Reflecting a strong commitment of Canadian virologists to science communication, CSV2018 featured the launch of Halifax’s first annual Soapbox Science event to enable public engagement with female scientists, and the live-taping of the 499th episode of the This Week in Virology (TWIV) podcast, hosted by Vincent Racaniello (Columbia University) and science writer Alan Dove. TWIV featured interviews of CSV co-founders Nathalie Grandvaux (Université de Montréal) and Craig McCormick (Dalhousie University), who discussed the origins and objectives of the new society; Ryan Noyce (University of Alberta), who discussed technical and ethical considerations of synthetic virology; and Kate O’Brien, who discussed vaccines and global health. Finally, because CSV seeks to provide a better future for the next generation of Canadian virologists, the symposium featured a large number of oral and poster presentations from trainees and closed with the awarding of presentation prizes to trainees, followed by a tour of the Halifax Citadel National Historic Site and an evening of entertainment at the historic Alexander Keith’s Brewery.

show abstract

Class A scavenger receptors mediate extracellular dsRNA sensing, leading to downstream antiviral gene expression in a novel American toad cell line, BufoTad

Cited by 16 publications

References 68 publications

Nephroblastoma in a Common Mudpuppy Necturus maculosus simultaneously Present with a Mollicute Bacterium of the Genus Acholeplasma

Nephroblastoma in a Common Mudpuppy Necturus maculosus simultaneously Present with a Mollicute Bacterium of the Genus Acholeplasma

Class A Scavenger Receptors Are Used by Frog Virus 3 During Its Cellular Entry

CSV2018: The 2nd Symposium of the Canadian Society for Virology

Contact Info

Product

Resources

About