Class II MHC Antigen-Expressing Cells in the Pulp Tissue of Human Deciduous Teeth Prior to Shedding.

Kannari, Naoko; Ohshima, Hayato; Maeda, Takeyasu; Noda, Tadashi; Takano, Yoshiro

doi:10.1679/aohc.61.1

Cited by 13 publications

(13 citation statements)

References 39 publications

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“…Internal resorption on the coronal and root dentin surrounding the pulp tissue is a rare pathological process in permanent human teeth (Trope et al 1994). In contrast, physiological root resorption commonly occurs in human deciduous teeth when numerous odontoclasts, which are the same cell type as osteoclasts in their ultrastructural features and functions (Sasaki 2003), gather along the pulpdentin border and extend their cellular processes into the dentinal tubules (Sasaki et al 1988;Sahara et al 1996;Kannari et al 1998;Linsuwanont et al 2002). Similar findings have been observed under the different pathological condition used in the present study and in a previous report, viz., numerous TRAP-positive osteoclast-lineage cells accumulate along the pulp-dentin border after tooth replantation (Shimazu et al 2002).…”

Section: Discussionsupporting

confidence: 90%

Histochemical and immunocytochemical study of hard tissue formation in dental pulp during the healing process in rat molars after tooth replantation

et al. 2006

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Dental pulp is assumed to possess the capacity to elaborate both bone and dentin matrix under the pathological conditions following tooth injury. This study was undertaken to clarify the mechanism inducing bone formation in the dental pulp by investigating the pulpal healing process, after tooth replantation, by micro-computed tomography (mu-CT), immunocytochemistry for heat-shock protein (HSP)-25 and cathepsin K (CK), and histochemistry for both alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). Under deep anesthesia, the upper right first molar of 4-week-old Wistar rats was extracted and immediately repositioned in the original socket. In control teeth at this age, the periphery of the coronal dental pulp showed intense ALP-positive and HSP-25-positive reactions, whereas there were no TRAP-positive or CK-positive cells. Tooth replantation weakened or terminated ALP-positive and HSP-25-positive reactions in the pulp tissue at the initial stages. At 3-7 days after operation, the ALP-positive region recovered from the root apex to the coronal pulp followed by HSP-25-positive reactions in successful cases showing tertiary dentin formation. In other cases, TRAP-positive and CK-positive cells appeared in the pulp tissue of the replanted tooth at postoperative days 5-10 and remained associated with the bone tissue after 12-60 days. Immunoelectron microscopy clearly demonstrated that CK-positive osteoclast-lineage cells made contact with mesenchymal cells with prominent nucleoli and well-developed cell organelles. These data suggest that the appearance of TRAP-positive and CK-positive cells is involved in the induction of bone tissue formation in dental pulp.

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Section: Discussionsupporting

confidence: 90%

Histochemical and immunocytochemical study of hard tissue formation in dental pulp during the healing process in rat molars after tooth replantation

et al. 2006

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“…Our previous reports have suggested that the class II MHC-positive cells perform diverse functions in odontoblast differentiation in the rat dental pulp under experimental conditions , in cementoblast-like cell differentiation in the human deciduous dental pulp prior to shedding (Kannari et al 1998), and in ordinary antigen presentation. Furthermore, human class II MHC-positive cells in the odontoblast layer and/or predentin might have some regulatory function on the homeostasis of odontoblasts under physiological conditions (Ohshima et al 1999a).…”

Section: Introductionmentioning

confidence: 99%

Responses of immunocompetent cells in the dental pulp to replantation during the regeneration process in rat molars

et al. 2000

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Responses of immunocompetent cells to tooth replantation during the regeneration process of the dental pulp in rat molars were investigated by immunocytochemistry using antibodies to class II major histocompatibility complex (MHC) molecules (OX6 antibody), monocyte/macrophage lineage cells (ED1 antibody) and protein gene product 9.5 (PGP 9.5), as well as by histochemical reaction for periodic acid-Schiff (PAS). Tooth replantation caused an increase in both the number of OX6- and ED1-positive cells and their immunointensity in the replanted pulp, but almost all PGP 9.5-immunoreactive nerves diminished in the initial stages. By postoperative day 3, many OX6- and ED -immunopositive cells had accumulated along the pulp-dentin border to extend their cytoplasmic processes into the dentinal tubules in successful cases. Once reparative dentin formation had begun after postoperative day 7, OX6- and ED1-immmunopositive cells became scattered in the odontoblast layer, while reinnervation was found in the coronal pulp. The temporal appearance of these immunocompetent cells at the pulp-dentin border suggests their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. On postoperative day 14, the replanted pulp showed three regeneration patterns: (1) reparative dentin, (2) bone-like tissue formation, and (3) an intermediate form between these. In all cases, PAS-reactive cells such as polymorphonuclear leukocytes (PML) and mesenchymal cells occurred in the pulp space. However, the prolonged stagnation of inflammatory cells was also discernible in the latter two cases. Thus, the findings on PAS reaction suggest that the migration of the dental follicle-derived cells into the pulp space and the subsequent total death of the proper pulpal cells are decisive factors for eliciting bone-like tissue formation in the replanted pulp.

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“…Therefore, the F4/80 molecule appears to be a multifunctional protein that plays an important role not only in the immune system of the dental papilla and pulp, but also in the differentiation of odontoblasts and matrix formation through interactions between dendritic cells and odontoblasts or extracellular matrix. Previous reports regarding class II MHC-positive dendritic cells in human dental pulp also suggested that these cells are involved in regulation and differentiation of odontoblasts (Kannari et al 1998;Ohshima et al 1995Ohshima et al , 1999.…”

Section: Discussionmentioning

confidence: 76%

“…The immunological aspects in pulpal tissue and the putative function of these cells were reviewed by Jontell et al (1998). It has been suggested that the class II MHC-expressing dendritic cells in the odontoblastic layer may regulate the function of odontoblasts and play a role in odontoblast differentiation in human and rat teeth (Ohshima et al 1995(Ohshima et al , 1999, and class II MHC-positive cells have been suggested to play an inductive role in differentiation, migration, and/or activation of odontoblasts and cementoblast-like cells during the resorption stage of human deciduous teeth (Kannari et al 1998). In addition, age-related change and delayed appearance of class II MHC-positive cells have been reported in dental pulp during development of rat incisors and molars (Jontell et al 1991;Okiji et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Tsuruga

Sakakura

Yajima

et al. 1999

Histochemistry and Cell Biology

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Dendritic cells and macrophages were examined in dental pulp during the postnatal development of mouse mandibular first molars, by immuno- and enzyme histochemistry. F4/80 antibody against dendritic cells and macrophages demonstrated labeled cells predominantly in and around the odontoblastic layer during tooth development from postnatal day 0 (PN0) to PN5. Labeling with Mac-1, Mac-2, and MOMA-2 antibodies against macrophages showed varied distribution patterns. Mac-1-positive cells were not detected in the dental pulp. Mac-2-positive cells appeared in the dental pulp at PN0, but not in or around the odontoblastic layer, and disappeared by PN3. A few MOMA-2-positive cells were detected in the dental pulp during the period examined. The F4/80-positive cells in and around the odontoblastic layer did not exhibit acid phosphatase or non-specific esterase activities. In addition, the F4/80-positive cells showed continued expression of Fcgamma receptor, but not class II major histocompatibility complex (MHC). Other antibodies against dendritic cells (NLDC-145, MIDC-8, and 33D1) did not label the F4/80-positive cells. We concluded that the F4/80-positive and class II MHC-negative cells in and around the odontoblastic layer may be immature dendritic cells in the early stages before eruption, weaning, and crucial exposure to antigenic stimuli. They may not only act primarily as immunosurveillance cells, but also play a role in a regulatory function and differentiation of odontoblasts.

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Class II MHC Antigen-Expressing Cells in the Pulp Tissue of Human Deciduous Teeth Prior to Shedding.

Cited by 13 publications

References 39 publications

Histochemical and immunocytochemical study of hard tissue formation in dental pulp during the healing process in rat molars after tooth replantation

Histochemical and immunocytochemical study of hard tissue formation in dental pulp during the healing process in rat molars after tooth replantation

Responses of immunocompetent cells in the dental pulp to replantation during the regeneration process in rat molars

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

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