Classical and post-genomic methods to study GIT function with emphasis on the pig

Metges, C. C.

doi:10.1016/j.livsci.2010.06.014

Cited by 7 publications

(1 citation statement)

References 117 publications

(184 reference statements)

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“…Also, no information exists on similar possible correlations of bacterial groups other than bifidobacteria. Finally, besides the potential interest of these additives in animal nutrition, the pig was also chosen as experimental animal in the current study because it is at present considered one of the best models for humans as far as intestinal physiology is concerned [9]. It is worth mentioning also that DFA are natural constituents of caramel [10] [11] and other sugar-rich foods, therefore being already present in the daily human diet.…”

Section: Introductionmentioning

confidence: 99%

A Di-D-Fructose Dianhydride-Enriched Caramel Modulates Pig Fecal Microbiota Composition

Rubio

Peinado²,

Echávarri³

et al. 2014

AiM

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A correlation has been previously described between bifidobacteria counts before and after the use of a dietary additive in human studies. However, to our knowledge no information on this topic has yet been reported in animals, and no information exists either on similar possible correlations of bacterial groups other than bifidobacteria. The potential prebiotic effects of di-D-fructose dianhydride (DFA)-enriched caramels have been previously reported in laboratory animals, poultry and pigs. In the present work, twelve growing male castrated pigs (41.8 ± 1.9 kg mean BW) were fed in succession on a control (no additive) or DFA-enriched caramel (20 g/kg) containing diet. Another group of 10 pigs (38.0 ± 3.7 kg mean BW) fed on a control diet without any additive was used as negative control. Bacterial log10 number of copies of the 16S rRNA gene was determined in fecal samples by using qPCR. Increased (P < 0.05) lactobacilli, Clostridium coccoides/Eubacterium rectale and bacteroides group log 10 number of copies were determined in fecal samples of pigs fed on the caramel containing diet compared with non-caramel controls. In addition, for all bacterial groups studied microbiological values co-variated with initial counts and, except for enterobacteria, variations in the fecal bacterial numbers after caramel supplementation correlated (P < 0.05) with the fecal numbers before supplementation. In conclusion, the supplementation of pig diets with DFA-enriched caramels induced significant increases in the fecal number of copies of bacterial groups regarded as beneficial, and variations in the fecal number of copies correlated with the initial fecal number of copies. * Corresponding author. L. A. Rubio et al.243

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Section: Introductionmentioning

confidence: 99%