Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Van, Turkey

Boynukara, Banur; Gülhan, Timur; Alişarli, Mustafa; Gürtürk, Kemal; Solmaz, Hasan

doi:10.1016/j.ijfoodmicro.2008.03.024

Cited by 48 publications

(43 citation statements)

References 10 publications

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“…Jørgensen et al (2005) reported that sec gene was most commonly detected in S. aureus from bovine bulk milk in Norway which is consistent with our results. Boynukara et al (2008), who examined 106 S. aureus strains, found that 25.5% of them had enterotoxin genes, among which 23.5% were positive for sea and 1.9% were positive for seb genes. In our study, we also detected S. aureus isolates with sea and seb genes.…”

Section: Discussionmentioning

confidence: 99%

“…Enterotoxins SEA, SEB, SEC, SED, called classic, cause more than 90% of cases of staphylococcal food poisoning (SFP). Milk and dairy products can be a source of enterotoxic staphylococci (Orwin et al 2001, Asao et al 2003, Boynukara et al 2008. Due to the growing importance of CNS as etiological agents of mastitis it is important to study the potential ability of production of enterotoxins by these bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

Piechota

Kot

Zdunek

et al. 2014

Polish Journal of Veterinary Sciences

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The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with-and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

Piechota

Kot

Zdunek

et al. 2014

Polish Journal of Veterinary Sciences

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“…In the case of an infected udder, S. aureus can contaminate milk during milking in a density ranging from 1-8 log CFU/ml, mostly about 4 log CFU/ml (Boynukara et al 2008).…”

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confidence: 99%

Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products

Medveďová¹,

Studeničová²,

Valı́k³

et al. 2014

Czech J. Food Sci.

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Medveďová A., Studeničová A., Valík Ľ., Horváthová Z. (2014): Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products. Czech J. Food Sci., 32: 337-341.We investigated the prevalence of enterotoxigenic S. aureus in the Slovakian dairy products and compared the reliability of different routine methods of identification. Out of 64 isolates, 44% were confirmed as S. aureus. There was only a little correlation in the confirmation by API Staph, VIT-Staphylococcus, and PCR detection. The PCR results revealed that 32% of S. aureus isolates possessed the selected gene for SEs enterotoxins (SEA-SEE), with sea as the most predominant gene. Neither seb nor sed genes were found throughout the collection. Additionally, the growth analysis of the isolates was performed in milk at 15°C. The growth parameters were very close to one another and were also compared with the data described in the global databases. These results provided evidence that the growth rate and lag phase duration can be determined with a high degree of reproducibility without regardless of the strain origin.

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“…The ability to cause a wide range of diseases may be associated with its production of a large spectrum of extracellular toxic compounds and other virulence factors such as toxic shock syndrome 1, exfoliative toxins and enterotoxins (Boynukara et al 2008). From a food safety point of view, the staphylococcal enterotoxin (SE) production is the most crucial problem which leads to the staphylococcal food poisoning outbreaks in humans as the third most common food intoxication in the world (HalpinDohnalek & Marth 1989;Baird-parker 2000;Ondrovčík 2003;Boynukara et al 2008;). The ability to produce one of the enterotoxins (SEA-SED) was observed in more than 20 % of the S. aureus strains examined by Boynukara et al (2008) and Vasiľ et al (2005) even more than 50% of the strains found by Akineden et al (2008) and Normanno et al (2007).…”

mentioning

confidence: 99%

“…From a food safety point of view, the staphylococcal enterotoxin (SE) production is the most crucial problem which leads to the staphylococcal food poisoning outbreaks in humans as the third most common food intoxication in the world (HalpinDohnalek & Marth 1989;Baird-parker 2000;Ondrovčík 2003;Boynukara et al 2008;). The ability to produce one of the enterotoxins (SEA-SED) was observed in more than 20 % of the S. aureus strains examined by Boynukara et al (2008) and Vasiľ et al (2005) even more than 50% of the strains found by Akineden et al (2008) and Normanno et al (2007). Furthermore, 65% up to 84 % of all of these strains were of human origin (Normanno et al 2007).…”

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confidence: 99%

Optimisation of Fermentative Parameters for GABA Enrichment by Lactococcus lactis

Lu¹,

Xie²,

Gu³

2009

Czech J. Food Sci.

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Staphylococcus aureus is a pathogenic bacterium that induces several of human illnesses. The staphylococcal enterotoxin (SE) production as the results of previous growth of toxigenic strains is the most crucial problem which may lead to the staphylococcal food poisoning outbreaks in humans. That is why the growth of three strains of Staphylococcus aureus was characterised in milk and modelled in dependence of temperature. For the lag phase duration of S. aureus 2064, the Davey model was used with the following result: ln(1/lag) = 1.973 -87.92/t + 285.09/t 2 (r 2 = 0.962).The dependence of the growth rate on incubation temperature was modelled by the Ratkowsky square root model and Gibson in sub-optimal and whole temperature range, respectively. The validation of both models showed high significance of the growth rate data fitting. The optimal temperature of t opt = 38.5°C was resulted from Gibson model for the S. aureus 2064 growth in milk. For practical purpose, the time necessary for the increase of S. aureus by 3 log counts was also calculated within the growth temperature range. These data may provide useful information e.g. for the producers using raw milk in their artisanal cheese practice as the specific strains were used in this study.

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Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Van, Turkey

Cited by 48 publications

References 10 publications

Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

Prevalence and growth dynamics of enterotoxinogenic Staphylococcus aureus isolates in Slovakian dairy products

Optimisation of Fermentative Parameters for GABA Enrichment by Lactococcus lactis

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