Classical Enterotoxin Genes of <i>Staphylococcus aureus</i> Isolated from the Raw Milk of Cows and Goats in Yogyakarta Indonesia

Widianingrum, Desy Cahya; Windria, Sarasati; Aziz, Fatkhanuddin; Salasia, Siti Isrina Oktavia

doi:10.2991/absr.k.210420.004

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…This SEB strain was isolated from humans in Yogyakarta, Central Java, Indonesia. S. aureus containing the gene encoding SEs A, B, C, G, H, and I have been isolated from the milk of dairy cows and goats [11,14]. Enterotoxins A, B, E, G, H, and I were Available at www.veterinaryworld.org/Vol.15/July-2022/22.pdf found in food products, cattle, and humans [15], in Central Java, Indonesia, indicating the potential for the spread of enterotoxins harmful to public health.…”

Section: Discussionmentioning

confidence: 99%

Cellular immune response of Staphylococcus aureus enterotoxin B in Balb/c mice through intranasal infection

et al. 2022

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Background and Aim: Staphylococcus aureus produces various superantigen exotoxins, including staphylococcal enterotoxin B (SEB). It causes fatal anaphylactic reactions and toxic shock. This study aimed to evaluate the reaction of leukocytes and histopathological changes in the respiratory organs of Balb/c mice after intranasal infection with enterotoxigenic S. aureus (SEB). Materials and Methods: The presence of the seb gene in S. aureus was established in this study using polymerase chain reaction-specific primer. Two groups of 8-week-old male Balb-c mice consist of six mice in each group. The treated group was infected with 50 μL and 100 μL of SEB intranasal on days 1 and 14, respectively. NaCl was administered in the second group and was considered as a control group. Blood samples were collected through the retro-orbital plexus on days 1, 4, 7, 14, and 22 after infections. Total cell counts were analyzed with an independent sample t-test and compared using the statistical package for the social sciences (SPSS) version 16.0 (IBM Corp., NY, USA). The infected tissues of the respiratory organ were observed descriptively and compared to the control group. Results: The seb gene with a molecular size of 478 bp, indicating the SEB strain, is present in S. aureus used in this study. Intranasal administration of SEB showed increased leukocytes, lymphocytes, monocytes, and eosinophils on day 22 post-infection. Significant leukocytosis was seen on days 6 and 14; lymphocytosis on days 1, 4, 6, and 16; and eosinophilia on days 6, 14, and 22 compared with the control group (p > 0.05). In contrast, the neutrophil decreased after an increase of immature band cells compared to the control group, indicating a severe acute infection with SEB. The lungs and trachea of the test group had an inflammatory cell accumulation in the respiratory organ. Conclusion: Intranasal route infection of S. aureus containing seb gene significantly induced the cellular immune response and caused pathological changes in the respiratory tissues of the Balb/c mice model. The hematological changes were aligned with marked pathological changes in the respiratory tract. Balb/c mice could be an excellent experimental model to study toxic and anaphylactic shock against SEB to define the future therapeutic agents.

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Section: Discussionmentioning

confidence: 99%

Cellular immune response of Staphylococcus aureus enterotoxin B in Balb/c mice through intranasal infection

et al. 2022

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“…It was also reported as the second most common in human isolates. The seh and sec genes appear to be the primary genes of isolated S. aureus from milk in the dairy farm [15] [17] and mastitis bovine [18]. In the mastitis case, seh gene was reported can induce bMES apoptosis on bovine mammary epithelial cells [18] and has been reported a cases of food poisoning caused by milk containing seh gene.…”

Section: Detection Of Enterotoxinmentioning

confidence: 99%

Multiplex PCR detection of the classical enterotoxin genes of Staphylococcus aureus in humans and animal isolates

Purwanasari,

Fitria,

Kusuma

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Staphylococcus aureus is a prevalent bacterial causative agent of bovine mastitis and human food illness by secreting enterotoxins. This study aims to identify the seven classic Staphylococcus aureus enterotoxin genes (SEA to SEH) isolated from humans and animals. A total of 173 human isolates and 14 mastitis (goat and cow) isolates were amplified with a 23S rRNA genes. The presence of classical enterotoxins genes (sea-seh) was amplified using multiplex polymerase chain reaction (m-PCR). Amplification of the SEs genes singly and in combination was observed from both isolates. Of 173 human isolates, the sec genes showed the highest prevalence of 112 isolates (65%), followed by the seh gene were 89 isolates (51%), 61 (35%) for seg gene and one (1%) for sea and see genes. The two genes in combination were also detected se (c, h), se (c, g), se (g, h)] and the three combination genes were se (c, g, h). Furthermore, all 14 animal isolates were positive for seh gene (100%), 11 (78%) for sec gene and 11 (78%) for combination se (c, h). Five classical enterotoxin genes (seh, sea, seg, sec, see) isolated from Staphylococcus aureus in humans were identified. Still, only seh and sec genes were identified from the animal origin. In the present study, identifying the genes encoding SE will contribute to recognize the prevalent of enterotoxins distribution among Staphylococcus aureus strains in samples of animals and human origin.

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“…Staphylococcus aureus (S. aureus) merupakan bakteri Gram positif penyebab mastitis (radang ambing) pada ternak perah seperti kambing peranakan etawah (PE) dan sapi perah (Boss et al, 2016;Ren et al, 2020;Widianingrum et al, 2021). Selain menyebabkan mastitis pada kambing PE dan sapi perah, diketahui susu segar merupakan sumber utama munculnya S. aureus dalam produk olahan susu (Verraes et al, 2015).…”

Section: Pendahuluanunclassified

“…Namun, yang perlu diperhatikan adanya kontaminasi patogen dan toksin yang diproduksi pada susu maupun produk turunannya yang dikonsumsi (Verraes et al, 2015). Beberapa penelitian sebelumnya telah melaporkan identifikasi S. aureus pada susu kambing PE (Windria et al, 2016;Aziz et al, 2020b;Widianingrum et al, 2021). Gen sey telah terdeteksi pada 28% isolat asal susu kambing PE pada penelitian ini, sehingga perlu diwaspadai potensi isolat S. aureus tersebut dalam menimbulkan keracunan makanan.…”

Section: Hasil Dan Pembahasanunclassified

Deteksi dan Analisis Filogenetik Staphylococcal Enterotoxin-Y Isolat Bakteri Staphylococcus aureus Asal Kambing Peranakan Etawah

Aziz,

Widianingrum,

Windria

et al. 2023

jvet

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Staphylococcus aureus diketahui sering menyebabkan radang ambing pada ternak perah dan memproduksi berbagai jenis enterotoksin yang berpotensi sebagai agen penyebab keracunan makanan melalui susu maupun produk olahan susu yang dikonsumsi. Staphylococcal enterotoxin Y (SEY) asal penyakit kulit pada manusia dilaporkan tahan terhadap uji pemanasan dan menyebabkan muntah pada hewan coba primata yang menunjukkan potensinya sebagai salah satu penyebab keracunan makanan. Pada penelitian ini, deteksi gen sey pada 18 isolat S. aureus asal kambing peranakan etawah (PE) dengan teknik polymerase chain reaction (PCR), kemudian dilakukan sekuensing gen sey dari isolat S. aureus MR6. Sekuens basa nukleotida ditranslasi menjadi protein menggunakan EMBOSS Transeq. Sekuens SEY MR6 selanjutnya dibandingkan dengan database genebank Bakteri SEY dan enterotoksin lain yang berasal dari isolat S. aureus asal manusia dan sapi menggunakan MultAlin. Pohon filogenetik dari protein SEY yang dibandingkan, dibuat menggunakan MEGA Software 5.0. Hasil PCR diketahui 28% isolat, positif gen sey. Analisis SEY dari isolat S. aureus kambing PE diketahui mempunyai persentase protein sekuens homologi sebesar 97,3% terhadap SEY asal isolat sapi perah dan manusia. Diketahui terdapat enam posisi residu asam amino yang berbeda antar SEY yang diperbandingkan. Analisis filogenetik menunjukkan kedekatan kekerabatan dengan SEY asal manusia, namun masih satu kluster dengan SEY asal sapi.

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Classical Enterotoxin Genes of Staphylococcus aureus Isolated from the Raw Milk of Cows and Goats in Yogyakarta Indonesia

Cited by 5 publications

References 20 publications

Cellular immune response of Staphylococcus aureus enterotoxin B in Balb/c mice through intranasal infection

Cellular immune response of Staphylococcus aureus enterotoxin B in Balb/c mice through intranasal infection

Multiplex PCR detection of the classical enterotoxin genes of Staphylococcus aureus in humans and animal isolates

Deteksi dan Analisis Filogenetik Staphylococcal Enterotoxin-Y Isolat Bakteri Staphylococcus aureus Asal Kambing Peranakan Etawah

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