Classical Light Scattering for the Determination of Absolute Molecular Weights and Gross Conformation of Biological Macromolecules

Harding, Stephen E.

doi:10.1385/0-89603-232-9:85

Cited by 7 publications

(4 citation statements)

References 8 publications

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“…The dn / dc is the specific refractive index increment value which depends upon the type of polysaccharide evaluated (because different types of molecules may have different dn / dc values), the solvent and the wavelength [ 13 ]. The dn / dc values obtained for pectin extracted using Celluclast 1.5 were relatively similar (0.185–0.191 mL/g).…”

Section: Resultsmentioning

confidence: 99%

Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin

Yuliarti

Matía-Merino

Goh

et al. 2011

IJMS

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The effects of Celluclast 1.5L concentration on the physicochemical characterization of gold kiwifruit pectin was evaluated. Varying the enzyme concentration affected the pectin yield and pectin physicochemical properties. The viscosity of extracted pectin was largely dependent on the enzyme concentration. Celluclast 1.5L with medium concentration exhibited the highest viscosity. Varying the enzyme concentration also influenced the molecular weight distribution. High molecular weight (Mw) pectin (1.65 × 106 g/mol) was obtained when the medium concentration was used. Overall, the study clearly reflects the importance of taking into consideration the amount of cellulytic enzyme added in order to determine the final quality of pectin.

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Section: Resultsmentioning

confidence: 99%

Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin

Yuliarti

Matía-Merino

Goh

et al. 2011

IJMS

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“…The light scattering data was analysed according to the arguments given below in order to find the dependence of the second virial coefficient on temperature, and by this means, the θ temperature. For solutions of macromolecules, the basic equation for the angular dependence of light scattering is the Debye-Zimm relation Harding, 1994;,…”

Section: Static Light Scatteringmentioning

confidence: 99%

Universal solvent quality crossover of the zero shear rate viscosity of semidilute DNA solutions

Pan

Nguyễn

Sridhar

et al. 2014

Journal of Rheology

105

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The scaling behaviour of the zero shear rate viscosity of semidilute unentangled DNA solutions, in the double crossover regime driven by temperature and concentration, is mapped out by systematic experiments. The viscosity is shown to have a power law dependence on the scaled concentration c/c * , with an effective exponent that depends on the solvent quality parameter z. The determination of the form of this universal crossover scaling function requires the estimation of the θ temperature of dilute DNA solutions in the presence of excess salt, and the determination of the solvent quality parameter at any given molecular weight and temperature. The θ temperature is determined to be T θ ≈ 15 • C using static light scattering, and the solvent quality parameter has been determined by dynamic light scattering. Typeset by REVT E Xand AIP 15 • C 20 • C 25 • C 30 • C 35 • C z 0 0.11 0.22 0.32 0.43 2.9 kbp c * 0.371 0.313 0.278 0.253 0.234 z 0 0.16 0.31 0.46 0.60 5.9 kbp c * 0.251 0.201 0.173 0.155 0.142 z 0 0.19 0.37 0.54 0.71 8.3 kbp c * 0.214 0.165 0.141 0.125 0.114 z 0 0.22 0.43 0.63 0.83 11.1 kbp c * 0.184 0.139 0.117 0.103 0.093 z 0 0.33 0.64 0.95 1.24 25 kbp c * 0.123 0.084 0.068 0.059 0.052 z 0 0.44 0.86 1.27 1.66 45 kbp c * 0.092 0.058 0.045 0.039 0.034 z 0 0.69 1.37 2.03 2.66 114.8 kbp c * 0.057 0.031 0.023 0.019 0.017 z 0 1.11 2.18 3.22 4.22 289 kbp c * 0.036 0.016 0.012 0.010 0.008 minutes at their maximum concentrations. This was done to prevent aggregation of long DNA chains [Heo and Larson, 2005]. The shear rate range of the instrument, under the applied geometry, is from 0.01 to 100 s −1 . At each shear rate, a delay of 30 seconds was employed so that the DNA chains have sufficient time to relax to their equilibrium state. Some typical relaxation times observed in dilute and semidilute solutions are given in Table I. At each temperature, a 30 minutes incubation time was employed for sample equilibration. III. SOLVENT QUALITY CROSSOVER OF THE ZERO SHEAR RATE VISCOSITY A. Zero shear rate viscosity of semidilute solutionsThe scaling behaviour of the zero shear rate viscosity of semidilute polymer solutions can be determined by measuring the viscosity as a function of concentration and temperature for a range of molecular weights, and then representing this behaviour in terms of the crossover variables z and c/c * . In order to do so, however, as discussed earlier in section I, it is first necessary to

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“…Another approach for dealing with the "dust problem" [56] when one is only interested in studying the protein drug monomer and/or its small oligomeric forms is to make LS measurements at very high angles. As already noted, the interference effects from very large scattering particles under these conditions is so high that their contribution to the measured scattered light will be significantly reduced.…”

Section: The "Dust Problem" In Sls and Dlsmentioning

confidence: 99%

Scattering Techniques for the Characterization of Biopharmaceuticals

Makowski

Berkowitz

Houde

2015

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

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Classical Light Scattering for the Determination of Absolute Molecular Weights and Gross Conformation of Biological Macromolecules

Cited by 7 publications

References 8 publications

Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin

Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin

Universal solvent quality crossover of the zero shear rate viscosity of semidilute DNA solutions

Scattering Techniques for the Characterization of Biopharmaceuticals

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