Classical music restored fertility status in rat model of premature ovarian failure

Abstract

Background: The restorative effect of classical music was assessed on the cyclophosphamide-induced animal model of premature ovarian failure (POF). Methods: Mozart’s piano classical music (K.448) was used for up to 4 and 8 weeks. Rats were exposed to music 6 h every day using a stereo system with a volume of 65–70 dB. Sera and ovarian tissue samples were collected for the evaluation of FSH, LH, and E2 and histopathological examination. At the same time points, samples were taken from the hypothalamus and hippocampus to monitor the expression of Ntrk2, Crh, and Pomc using real-time PCR. Mating trial was performed to evaluate the fertility status of POF rats. Results: Histopathological examination revealed a significant increase (p < 0.05) in the numbers of morphologically normal follicles at all the developmental stages in POF rats after music therapy compared to the POF group (p < 0.05). Music therapy decreased FSH and LH levels to near-to-normal levels conidied with elevation of E2 (p < 0.05). Ntrk2, Crh, and Pomc expressions were down-regulated in POF rats. Music therapy increasaed the expression of Ntrk2 in the hypothalamus of POF rats (p < 0.05). In contrast, Crh and Pomc failed to reach the detection limit before intervention and four weeks after the intervention however, these genes were expressed eight weeks after music therapy. Fertility status was increased (p < 0.05) in terms of litter size in POF rats after being exposed to music compared to the non-treated POF control group (p < 0.05). Conclusion: Results showed that music can exert therapeutic effects on POF rats via the alteration of sex-related hormones.

The physiological functions and therapeutic potential of exosomes during the development and treatment of polycystic ovary syndrome

“…Notably, the conditioned medium is considered as a putative and rich source of exosomes in clinical settings, and the importance of cell vs. cell-free therapy has been investigated in the field of reproductive biology both in human and animal settings (Jiao et al, 2020;Zhang et al, 2021b;Abd-Elwahab et al, 2023;Amiri et al, 2023). For example, recent findings discussed the regenerative potential of exosomes in premature ovarian condition and even azoospermia in animal settings (Li et al, 2021c;Nazdikbin Yamchi et al, 2023;Park et al, 2023). Therefore, exosomes and their non coding RNAs may serve as diagnostic and therapeutic targets for PCOS, especially MSC-derived exosomes as a new method for treating infertility caused by PCOS (Figure 9).…”

Comparison the therapeutic effects of bone marrow CD144+ endothelial cells and CD146+ mesenchymal stem cells in POF rats

“…Serum levels of FSH, luteinizing hormone (LH), and E2 were detected in blood samples taken from the hearts of rats under deep anesthesia according to our previous work. 33 Serum samples were then collected after centrifugation at 400g for 20 minutes. Enzyme-linked immunosorbent assay (ELISA) was used for measuring FSH (334-096-4, Monobind), LH (0234-96, Monobind), and E2 (4925-300A, Monobind) levels.…”

Plasma‐derived from human umbilical cord blood restores ovarian function and improves serum reproductive hormones levels in mice with premature ovarian insufficiency (POI) through cytokines and growth factors

“…Pathologically, POI is a common reproductive disorder affecting 1%–5% of women (Webber et al, 2016). Its primary features include menstrual disorder, increased gonadotropin (Gn) and follicle‐stimulating hormone (FSH) levels and decreased estradiol and anti‐Müllerian hormone (AMH) levels before 40 years of age (Jiao et al, 2022; Nazdikbin Yamchi et al, 2022). Currently, there are very limited options for treating declined ovarian function.…”