Classification and characterization of the rice ?-amylase multigene family

Huang, Ning; Sutliff, Thomas D.; Litts, James C.; Rodriguez, Raymond L.

doi:10.1007/bf00016499

Cited by 184 publications

(147 citation statements)

References 48 publications

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“…Induction of a-amylase gene expression is controlled through the interaction of regulatory proteins with cis-acting elements in the promoters of the a-amylase genes. Nucleotide sequence comparisons of severa1 rice, barley, and wheat a-amylase genes revealed a highly conserved sequence element (TAACRRA) in the promoters of these genes (Huang et al, 1990b). (DNA sequence ambiguity code: R = A or G.) This element is required for GA induction of a-amylase gene expression, hence the name Gibberellic Acid Response Element or GARE box (Rogers et (Ranjhan et al, 1991), but no clone for this putative 10th meniber of the gene family has been isolated to date.…”

Section: The Ca Slgnal Stimulates Cereal Seedling Source Metabolismmentioning

confidence: 99%

Metabolite Signals Regulate Gene Expression and Source/Sink Relations in Cereal Seedlings

Thomas¹,

Rodriguez²

1994

Plant Physiol.

122

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Section: The Ca Slgnal Stimulates Cereal Seedling Source Metabolismmentioning

confidence: 99%

Metabolite Signals Regulate Gene Expression and Source/Sink Relations in Cereal Seedlings

Thomas¹,

Rodriguez²

1994

Plant Physiol.

122

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“…GUS activity was hardly detected in the corresponding tissues of the negative control plants (data not shown). Consistent with the GUS activity levels, the promoter region contains several important elements, such as an amylase-box, CARE, GARE, GCN4-motif, ACGT motif and AACA motif, which are involved in seed-specific and normal embryo-specific expression [24,[28][29][30][31]39]. Quantitative GUS assays showed that the highest expression was in Functional analysis of a GhGlcAT1 promoter 180 npg the anthers, with relatively high expression in the stigmas and styles.…”

Section: Histochemical and Quantitative Analysis Of Gus Activitymentioning

confidence: 89%

“…Furthermore, regulatory elements with homology to those identified in other seed/endospermspecific genes were found in the pGhGlcAT1 region such as an amylase-box, E-box, CARE, GARE, GCN4-motif, ACGT motif and AACA motif ( Table 1). The amylase-box was required for high expression of the gene in the seeds [24], while the E-box was shown to be bound by bHLH (basic helix-loop-helix) transcription factors and to regulate the seed-specific expression of seed storage protein genes and the expression of genes involved in anthocyanin biosynthesis [25][26][27]. The CARE and GARE boxes were required during seed germination in Arabidopsis and rice [28,29].…”

Section: Sequence Characterization Of the Ghglcat1 Promoter Regionmentioning

confidence: 99%

Functional analysis of a cotton glucuronosyltransferase promoter in transgenic tobaccos

Liu

2006

Cell Res

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The 5' fragment (1 647 bp) of the cotton glucuronosyltransferase gene (GhGlcAT1) was transcriptionally fused to the b-glucuronidase (GUS) gene, and functionally analyzed for important regulatory regions controlling gene expression in transgenic tobacco plants. GUS activity analysis revealed that the full-length promoter drives efficient expression of the GUS gene in the root cap, seed coat, pollen grains and trichomes. Exposure of the transgenic tobacco to various abiotic stresses showed that the promoter was mainly responsive to the sugars (glucose and sucrose) as well as gibberellic acid. Progressive upstream deletion analyses of the promoter showed that the region from -281 to +30 bp is sufficient to drive strong GUS expression in the trichomes of shoot, suggesting that the 311 bp region contains all cis-elements needed for trichome-specific expression. Furthermore, deletion analysis also revealed that the essential cis-element(s) for sucrose induction might be located between -635 and -281 bp. In addition, sequence analysis of the regulatory region indicated several conserved motifs among which some were shared with previously reported seed-specific elements and sugarresponsive elements, while others were related with trichome expression. These findings indicate that a 1 647-bp fragment of the cotton GhGlcAT1 promoter contains specific transcription regulatory elements, and provide clues about the roles of GhGlcAT1 in cotton fiber development. Further analyses of these elements will help to elucidate the molecular mechanisms regulating the expression of the GhGlcAT1 gene during fiber elongation.

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“…Deletion of the regions containing these motifs reduces GAupregulated expression (Huttly, Phillips & Tregear, 1992). The rice RAmy 1A and Osamy-c genes have TRE-like motifs adjacent to their pyrimidine boxes (Huang et al, 1990 ;, and for Osamy-c, nuclear protein has been shown to bind to this region. Thus both the O2S and TRE motifs appear to be specific for the low-pI amylases, and to be involved in the GA-induction system.…”

Section: Transcriptional Regulationmentioning

confidence: 99%

Gibberellins: regulating genes and germination

Ritchie

Gilroy

1998

New Phytologist

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The range of processes regulated by gibberellins (GAs) covers all aspects of the life history of the plant from seed germination to vegetative growth and flowering. In seeds there has been an intensive search, using the techniques of both biochemistry and cell biology, for the regulatory molecules linking GA perception to gene regulation and the events of germination. Although a GA receptor has yet to be identified, the site of perception has been localized to the plasma membrane. Calmodulin, Ca2+ and cGMP have also been identified as elements of the GA signal transduction pathway. These regulators parallel many of the signalling elements identified in the transduction of other signals such as phytochrome and ABA. Studies of GA‐regulated gene expression, principally of the α‐amylases of cereal aleurone, have identified core GA‐responsive promoter elements, such as the gibberellin response element (GARE), box‐1 and pyrimidine boxes, as well as elements that may lend specificity to GA‐regulated expression, such as the Opaque‐2‐similar element (O2S), and TRE and CRE motifs. One of the most striking features of all of these studies of the molecular basis of GA action is the interaction of GA‐dependent regulatory elements with those of other factors such as ABA. GA‐response elements also appear to be conserved between disparate GA‐response systems. For example, Myb transcription factors appear to regulate a multitude of GA‐induced genes in cereal aleurone as well as to alter GA responses when expressed in Arabidopsis. Thus the study of GA signal transduction and response systems is highlighting the conservation of regulatory elements used by plants. These common factors, used by distinct signal transduction systems, provide a molecular basis for the integration of the GA signal with other growth regulators that is the hallmark of plant growth and development.

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Classification and characterization of the rice ?-amylase multigene family

Cited by 184 publications

References 48 publications

Metabolite Signals Regulate Gene Expression and Source/Sink Relations in Cereal Seedlings

Metabolite Signals Regulate Gene Expression and Source/Sink Relations in Cereal Seedlings

Functional analysis of a cotton glucuronosyltransferase promoter in transgenic tobaccos

Gibberellins: regulating genes and germination

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